Heterologous expression and characterization of an Arabidopsis β-l-arabinopyranosidase and α-d-galactosidases acting on β-l-arabinopyranosyl residues

Imaizumi, Chiemi; Tomatsu, Harumi; Kitazawa, Kiminari; Yoshimi, Yoshihisa; Shibano, Seiji; Kikuchi, Kaoru; Yamaguchi, Masatoshi; Kaneko, Satoshi; Tsumuraya, Yoichi; Kotake, Toshihisa

doi:10.1093/jxb/erx279

Cited by 22 publications

(23 citation statements)

References 73 publications

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“…The amino acid sequences of well-characterized galactinol- and RFO synthase enzymes of Arabidopsis thaliana (AT2G47180.1, AT1G56600.1, AT1G09350.1, AT1G60470.1, AT5G23790.1, AT4G26250.1, AT1G60450.1, AT5G30500.1, NP_198855.1, NP_192106.3), Cicer arietinum (AMP59727.1, AMP59729.1), Pisum sativum (CAD20127.2), Oryza sativa (XP_015621501.1), Zea mays (NP_001354805.1) and Vigna angularis (CAB64363.1) were initially used as queries against the protein and gene databases of the National Center for Biotechnology Information (NCBI) and Phytozome databases to search for the orthologs in P. vulgaris and G. max using BLASTP and TBLASTN ( Table S1 ) [ 23 , 40 , 51 , 52 , 60 , 61 , 62 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 ]. The resulting gene/protein sequences were further used to perform a combination of BLASTP, TBLASTN and BLASTN to find all potential galactinol and RFO biosynthetic genes in P. vulgaris and G. max .…”

Section: Methodsmentioning

confidence: 99%

Identification and Expression Analysis of the Genes Involved in the Raffinose Family Oligosaccharides Pathway of Phaseolus vulgaris and Glycine max

Koning

Kiekens

Toili

et al. 2021

Plants

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Raffinose family oligosaccharides (RFO) play an important role in plants but are also considered to be antinutritional factors. A profound understanding of the galactinol and RFO biosynthetic gene families and the expression patterns of the individual genes is a prerequisite for the sustainable reduction of the RFO content in the seeds, without compromising normal plant development and functioning. In this paper, an overview of the annotation and genetic structure of all galactinol- and RFO biosynthesis genes is given for soybean and common bean. In common bean, three galactinol synthase genes, two raffinose synthase genes and one stachyose synthase gene were identified for the first time. To discover the expression patterns of these genes in different tissues, two expression atlases have been created through re-analysis of publicly available RNA-seq data. De novo expression analysis through an RNA-seq study during seed development of three varieties of common bean gave more insight into the expression patterns of these genes during the seed development. The results of the expression analysis suggest that different classes of galactinol- and RFO synthase genes have tissue-specific expression patterns in soybean and common bean. With the obtained knowledge, important galactinol- and RFO synthase genes that specifically play a key role in the accumulation of RFOs in the seeds are identified. These candidate genes may play a pivotal role in reducing the RFO content in the seeds of important legumes which could improve the nutritional quality of these beans and would solve the discomforts associated with their consumption.

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Section: Methodsmentioning

confidence: 99%

Identification and Expression Analysis of the Genes Involved in the Raffinose Family Oligosaccharides Pathway of Phaseolus vulgaris and Glycine max

Koning

Kiekens

Toili

et al. 2021

Plants

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“…AtAPSE (β-l-ARAPASE) is a β-l-arabinopyranosidase in the GH27 family. In an apse mutant, the amount of β-l-Arap residues of AGPs was higher compared to wild type, confirming its activity (Imaizumi et al, 2017).…”

Section: Glycoside Hydrolases: Cutting All the Sugarmentioning

confidence: 72%

Three Decades of Advances in Arabinogalactan-Protein Biosynthesis

et al. 2020

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Arabinogalactan-proteins (AGPs) are a large, complex, and highly diverse class of heavily glycosylated proteins that belong to the family of cell wall hydroxyproline-rich glycoproteins. Approximately 90% of the molecules consist of arabinogalactan polysaccharides, which are composed of arabinose and galactose as major sugars and minor sugars such as glucuronic acid, fucose, and rhamnose. About half of the AGP family members contain a glycosylphosphatidylinositol (GPI) lipid anchor, which allows for an association with the outer leaflet of the plasma membrane. The mysterious AGP family has captivated the attention of plant biologists for several decades. This diverse family of glycoproteins is widely distributed in the plant kingdom, including many algae, where they play fundamental roles in growth and development processes. The journey of AGP biosynthesis begins with the assembly of amino acids into peptide chains of proteins. An N-terminal signal peptide directs AGPs toward the endoplasmic reticulum, where proline hydroxylation occurs and a GPI anchor may be added. GPI-anchored AGPs, as well as unanchored AGPs, are then transferred to the Golgi apparatus, where extensive glycosylation occurs by the action of a variety glycosyltransferase enzymes. Following glycosylation, AGPs are transported by secretory vesicles to the cell wall or to the extracellular face of the plasma membrane (in the case of GPI-anchored AGPs). GPI-anchored proteins can be released from the plasma membrane into the cell wall by phospholipases. In this review, we present an overview of the accumulated knowledge on AGP biosynthesis over the past three decades. Particular emphasis is placed on the glycosylation of AGPs as the sugar moiety is essential to their function. Recent genetics and genomics approaches have significantly contributed to a broader knowledge of AGP biosynthesis. However, many questions remain to be elucidated in the decades ahead.

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“…BnaA05g18270D in the mapping interval, is homologous to AT3G56310.1, which encodes a member of the glycoside hydrolase family 27 that is an α-galactosidase (AGAL3). AGAL3 has a signal peptide at the N-terminus and is likely to be responsible for the hydrolysis of the β-l-arabinopyranoside residues in A. thaliana [48]. AGAL3 also has not been reported to be related to leaf shaping.…”

Section: Resultsmentioning

confidence: 99%

Fine mapping of an up-curling leaf locus (BnUC1) in Brassica napus

et al. 2019

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Background Leaf shape development research is important because leaf shapes such as moderate curling can help to improve light energy utilization efficiency. Leaf growth and development includes initiation of the leaf primordia and polar differentiation of the proximal-distal, adaxial-abaxial, and centrolateral axes. Changes in leaf adaxial-abaxial polarity formation, auxin synthesis and signaling pathways, and development of sclerenchyma and cuticle can cause abnormal leaf shapes such as up-curling leaf. Although many genes related to leaf shape development have been reported, the detailed mechanism of leaf development is still unclear. Here, we report an up-curling leaf mutant plant from our Brassica napus germplasm. We studied its inheritance, mapped the up-curling leaf locus BnUC1 , built near-isogenic lines for the Bnuc1 mutant, and evaluated the effect of the dominant leaf curl locus on leaf photosynthetic efficiency and agronomic traits. Results The up-curling trait was controlled by one dominant locus in a progeny population derived from NJAU5734 and Zhongshuang 11 (ZS11). This BnUC1 locus was mapped in an interval of 2732.549 kb on the A05 chromosome of B. napus using Illumina Brassica 60 K Bead Chip Array. To fine map BnUC1 , we designed 201 simple sequence repeat (SSR) primers covering the mapping interval. Among them, 16 polymorphic primers that narrowed the mapping interval to 54.8 kb were detected using a BC 6 F 2 family population with 654 individuals. We found six annotated genes in the mapping interval using the B. napus reference genome, including BnaA05g18250D and BnaA05g18290D, which bioinformatics and gene expression analyses predicted may be responsible for leaf up-curling. The up-curling leaf trait had negative effects on the agronomic traits of 30 randomly selected individuals from the BC 6 F 2 population. The near-isogenic line of the up-curling leaf (ZS11-UC1) was constructed to evaluate the effect of BnUC1 on photosynthetic efficiency. The results indicated that the up-curling leaf trait locus was beneficial to improve the photosynthetic efficiency. Conclusions An up-curling leaf mutant Bnuc1 was controlled by one dominant locus BnUC1 . This locus had positive effects on photosynthetic efficiency, negative effects on some agronomic traits, and may help to increase planting density in B. napus . Electronic supplementary material The online version of this article (10.1186/s12870-019-1938-0) contains supplementary material, which is available to authorized users.

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Heterologous expression and characterization of an Arabidopsis β-l-arabinopyranosidase and α-d-galactosidases acting on β-l-arabinopyranosyl residues

Abstract: Through the analysis of mutants and recombinant enzymes expressed in Pichia yeast, an Arabidopsis β-l-arabinopyranosidase and α-d-galactosidases are shown to be responsible for the hydrolysis of β-l-arabinopyranosyl residues.

Cited by 22 publications

References 73 publications

Identification and Expression Analysis of the Genes Involved in the Raffinose Family Oligosaccharides Pathway of Phaseolus vulgaris and Glycine max

Identification and Expression Analysis of the Genes Involved in the Raffinose Family Oligosaccharides Pathway of Phaseolus vulgaris and Glycine max

Three Decades of Advances in Arabinogalactan-Protein Biosynthesis

Fine mapping of an up-curling leaf locus (BnUC1) in Brassica napus

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