Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor

Lee, Seu-Na; Jang, Hong-Su; Woo, Jae Sung

doi:10.1007/978-1-0716-2879-9_10

Cited by 1 publication

(2 citation statements)

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“…The scientific community has made notable strides toward safer and more effective CRISPR-based therapies. Recent advancements have prioritized precise sgRNA design through programmability; engineering modified Cas9 variants, such as base or prime editors; and the development of sophisticated non-viral delivery methods, placing strong emphasis on safety and efficacy [ 26 , 46 , 47 , 107 , 108 ]. Furthermore, implementing spatiotemporal control technologies within the CRISPR system adds another layer of safety and accuracy to the process [ 67 , 102 ].…”

Section: Navigating Safety Concerns In Crispr Gene Editing For Clinic...mentioning

confidence: 99%

“…Several innovative and personalized cancer treatment strategies have been developed using the precision and versatility of CRISPR, potentially overcoming one of cancer’s most notorious strategies for evading the immune system [ 22 , 23 , 24 ]. As research continues to advance, the possibilities of CRISPR-based cancer therapies seem boundless, providing renewed hope to cancer patients and their families worldwide [ 11 , 22 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

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CRISPR/Cas9-Mediated Customizing Strategies for Adoptive T-Cell Therapy

Park,

Kang,

Shim

2024

Pharmaceutics

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Clustered regularly interspaced short palindromic repeat-associated protein Cas9 (CRISPR/Cas9) technology is at the forefront of cancer immunotherapy innovation, offering precise and personalized treatment strategies. In this review, we discuss CRISPR/Cas9’s ability to precisely edit the genome, its impact on immune checkpoint control, and its application in immune cell engineering, where it surpasses traditional gene editing techniques. Originally inspired by bacterial defense mechanisms, this technology has made great strides in cancer immunotherapy as a mechanism to specifically target the PD-1/PD-L1 pathway in immune checkpoint blockades. In addition, CRISPR/Cas9 plays an important role in cancer treatment by facilitating genetic modifications to enhance the properties of adoptive cell therapy, optimizing the therapeutic potential of this approach. This review provides an overview of the development of CRISPR/Cas9, its important role in immune checkpoint control, applications in immune cell engineering, and the current status of clinical trials. However, safety concerns related to off-target effects and unintended mutations require continued research and caution. Continued advances in CRISPR technology hold the promise of revolutionizing the cancer treatment paradigm, providing personalized and effective therapies for patients with various types of cancer.

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