Heterologous expression of a hydrogenase gene in Enterobacter aerogenes to enhance hydrogen gas production

Zhao, Jun; Wu, Song; Cheng, Jun; Zhang, Chuan‐Xi

doi:10.1007/s11274-009-0139-7

Cited by 23 publications

(6 citation statements)

References 31 publications

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“…E. cloacae CICC10017 was purchased from China Center of Industrial Culture Collection, while E. aerogenes ATCC13408 was purchased from China General Microbiological Culture Collection Center. The genes of several hydrogenase were over‐expressed in E. cloacae CICC10017 and E. aerogenes ATCC13408, as described in our previous study . Four recombinant strains including E. cloacae /HPP, E. aerogenes /HycE, E. aerogenes /HycG, and E. aerogenes /HoxEFUYH were obtained.…”

Section: Methodsmentioning

confidence: 99%

“…PCC 6803 were cloned and heterologously expressed in E. aerogenes ATCC13408 to get the recombinant strain of E. aerogenes /HoxEFUYH. The cultivation process and the growth medium for the hydrogen producing bacteria were also described in our previous study . The experimental design is shown in Table .…”

Section: Methodsmentioning

confidence: 99%

“…It is feasible and effective to enhance the hydrogen production of Enterobacter strains via gene engineering . However, most of the feedstocks for hydrogen fermentation using the genetically modified bacteria were glucose , which is impossible to use for industrial production. To our best knowledge, few studies discussed the hydrogen producing characteristics of genetically modified bacteria from biomass in literature to date.…”

Section: Introductionmentioning

confidence: 99%

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Improving fermentative hydrogen production from water hyacinth with genetically modified bacteria

Cheng

Ding

et al. 2017

Env Prog and Sustain Energy

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Water hyacinth pretreated with microwave-assisted dilute acid was used for fermentation to produce hydrogen using four kinds of genetically modified bacteria for the first time in this study. The genes of several hydrogenase were overexpressed in Enterobacter cloacae CICC10017 and Enterobacter aerogenes ATCC13408. Four recombinant strains including E. cloacae/HPP, E. aerogenes/HycE, E. aerogenes/ HycG, and E. aerogenes/HoxEFUYH were obtained. Hydrogen yields from water hyacinth were investigated, which were enhanced using the recombinant strains. When water hyacinth was pretreated by microwave at 1408C for 15 min with 1% H 2 SO 4 and then hydrolyzed by cellulase, the reducing sugar yield of 49.4 g/100 g hyacinth was 91.4% of the theoretical reducing sugar yield. Among the four genetically modified bacteria, E. cloacae/HPP gave the highest hydrogen yield of 74.9 mL/g-total volatile solids, which was 50% higher than that given by the wild strain from pretreated water hyacinth.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Improving fermentative hydrogen production from water hyacinth with genetically modified bacteria

Cheng

Ding

et al. 2017

Env Prog and Sustain Energy

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“…Various efforts have been made to enhance the biohydrogen production via genetic engineering application. Genetically modified bacteria such as E. Coli (140,141) Clostridium sp. (142,143) and some species of Enterobacter (144) were successfully used for high yield of biohydrogen.…”

Section: In Microbesmentioning

confidence: 99%

A critical review on factors influencing fermentative hydrogen production

Kothari¹

2017

Front Biosci

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Biohydrogen production by dark fermentation of different waste materials is a promising approach to produce bio-energy in terms of renewable energy exploration. This communication has reviewed various influencing factors of dark fermentation process with detailed account of determinants in biohydrogen production. It has also focused on different factors such as improved bacterial strain, reactor design, metabolic engineering and two stage processes to enhance the bioenergy productivity from substrate. The study also suggest that complete utilization of substrates for biological hydrogen production requires the concentrated research and development for efficient functioning of microorganism with integrated application for energy production and bioremediation. Various studies have been taken into account here, to show the comparative efficiency of different substrates and operating conditions with inhibitory factors and pretreatment option for biohydrogen production. The study reveals that an extensive research is needed to observe field efficiency of process using low cost substrates and integration of dark and photo fermentation process. Integrated approach of fermentation process will surely compete with conventional hydrogen process and replace it completely in future.

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“…A produção fermentativa de hidrogênio nos últimos anos tem sido ativamente estudada devido a sua flexibilidade em utilizar águas residuais ou resíduos sólidos como fonte de recurso renovável, caracterizando o processo como ecologicamente correto (MUDHOO; SÁNCHEZ, 2011;LIN et al, 2012;CHA, 2013). A elucidação da informação genética das hidrogenases tem permitido numerosas abordagens para a expressão dessas enzimas em hospedeiros heterólogos na tentativa de otimizar a produção de hidrogênio (ZHAO et al, 2010). E a exploração de sequências obtidas a partir da genômica e metagenômica auxiliará a identificação de novas hidrogenases potencialmente úteis.…”

Section: Hidrogenases: Produção Fermentativa De Biohidrogêniounclassified

Diversidade e prospecção de metagenoma microbiano em fermentadores de biogás produzindo H2

Tomazetto¹,

Merzel²

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Hydrogen appears to be the most promising candidate for the replacement of fossil fuel due to its potentially higher efficiency of conversion to usable power and no toxic emission production.The production of hydrogen from organic wastes is performed through the anaerobic digestion, making it an environmentally friendly alternative for satisfying future hydrogen demands.Nonetheless, the microorganisms and metabolic processes involved are far from being exhaustively characterized. In this work, samples of a domestic sewage treatment plant were analyzed in two complementary studies aiming at the characterization of its phylogenetic diversity and the description of new hydrogenases. The first one, combined the analysis of 16S rRNA and [FeFe]-hydrogenase (hydA) genes with statistical tools to estimate richness and diversity of the prokaryotic community at the phylogenetic and functional levels. Phylogenetic analysis showed that all archaeal sequences were affiliated with yet uncultured Euryarchaeota and that Proteobacteria were the most predominant and diversified phylogenetic group within the bacterial library. The putative hydA sequences were identified as hitherto undetected [Fe-Fe]hydrogenase gene sequences. Diversity statistical analysis confirmed a great richness and diversity of bacterial and hydA sequences retrieved from the sewage sludge sample. In the second approach, a fosmid metagenomic library was constructed and analyzed employing 454pyrosequencing technology, resulting in approximately 218 Mb of data. Three different classifiers applied allowed a broad overview of the most abundant taxonomic groups due to a huge number of metagenome reads remained unidentified. However, taxonomic analysis revealed Gammaproteobacteria and Deltaproteobacteria, respectively, as the most abundant classes,

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Heterologous expression of a hydrogenase gene in Enterobacter aerogenes to enhance hydrogen gas production

Cited by 23 publications

References 31 publications

Improving fermentative hydrogen production from water hyacinth with genetically modified bacteria

Improving fermentative hydrogen production from water hyacinth with genetically modified bacteria

A critical review on factors influencing fermentative hydrogen production

Diversidade e prospecção de metagenoma microbiano em fermentadores de biogás produzindo H2

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