Heterologous expression of biologically active Mambalgin-1 peptide as a new potential anticancer, using a PVX-based viral vector in Nicotiana benthamiana

Khezri, Ghaffar; Rouz, Bahram Baghban Kohneh; Ofoghi, Hamideh; Davarpanah, Seyed Javad

doi:10.1007/s11240-020-01838-x

Cited by 6 publications

(3 citation statements)

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“…Agrobacterium -transformed cells in Nicotiana benthamiana by gene Mambalgin-1 had significant cytotoxicity against SH-SY5Y cancer cells (nerve cells). Due to this, it may be employed as an anticancer agent ( Khezri et al, 2020 ). Therefore, functional genomics research is essential to understanding how different genes involved in biosynthetic processes are expressed, thereby helping identify those genes whose heterologous expression can enhance the production of beneficial anticancer secondary metabolites.…”

Section: Biotechnological and Metabolic Engineering Used To Enhance A...mentioning

confidence: 99%

Biosynthesis of anticancer phytochemical compounds and their chemistry

Dogra

Kumar

2023

Front. Pharmacol.

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Cancer is a severe health issue, and cancer cases are rising yearly. New anticancer drugs have been developed as our understanding of the molecular mechanisms behind diverse solid tumors, and metastatic malignancies have increased. Plant-derived phytochemical compounds target different oncogenes, tumor suppressor genes, protein channels, immune cells, protein channels, and pumps, which have attracted much attention for treating cancer in preclinical studies. Despite the anticancer capabilities of these phytochemical compounds, systemic toxicity, medication resistance, and limited absorption remain more significant obstacles in clinical trials. Therefore, drug combinations of new phytochemical compounds, phytonanomedicine, semi-synthetic, and synthetic analogs should be considered to supplement the existing cancer therapies. It is also crucial to consider different strategies for increased production of phytochemical bioactive substances. The primary goal of this review is to highlight several bioactive anticancer phytochemical compounds found in plants, preclinical research, their synthetic and semi-synthetic analogs, and clinical trials. Additionally, biotechnological and metabolic engineering strategies are explored to enhance the production of bioactive phytochemical compounds. Ligands and their interactions with their putative targets are also explored through molecular docking studies. Therefore, emphasis is given to gathering comprehensive data regarding modern biotechnology, metabolic engineering, molecular biology, and in silico tools.

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Section: Biotechnological and Metabolic Engineering Used To Enhance A...mentioning

confidence: 99%

Biosynthesis of anticancer phytochemical compounds and their chemistry

Dogra

Kumar

2023

Front. Pharmacol.

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“…The supernatant was centrifuged at 5000 rpm and 4°C for 15 minutes. The supernatant was transferred to a clean microtube and stored at minus 20°C (Figure 9) [13].…”

Section: Confirmation Of Masp1 Protein Production In Transgenic Cotto...mentioning

confidence: 99%

“…The membrane was then incubated in the staining solution. In order to stop staining, the membrane was placed in distilled water (Figure 10) [13].…”

Section: Western Blot Analysismentioning

confidence: 99%

Transgenic Cotton Plants Expressing Spidroin Gene Presenting Increased Fiber Quality

Davarpanah¹

2021

PDRAJ

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Introduction: Spider silks are the strongest and most elastic fibers known in the nature. Cotton is also one of the most important crops in the world which increasing the quality of its fibers is of great importance in the textile industry, so the production of longer and stronger cotton fibers is of great importance. The aim of this study was to produce transgenic cotton fibers containing MaSp1 cobweb protein. Material and Methods: A synthetic construct was designed based on a selected fragment of Latrodectushesperus MaSp1 gene. The gene was then cloned twice inpCAMBIA1304 binary vector using different restriction enzymes (NcoI and NheI; EcoR1 and NheI). Agrobacterium tumefaciens LBA4404strain was transformed by recombinant vectors which are used to transform cotton plants. The accuracy of the results was confirmed using PCR, hygromycin resistance gene and recombinant protein expression. Finally, the fiber strength of transgenic cotton was measured by two vectors. Result: MaSp1 synthetic gene was transferred to the plant meristem. The study of transgenic plants in the next generations confirmed the accuracy of gene transfer and expression of recombinant protein. Conclusion: Considering the economic role of cotton in the world and also having the third rank among the industrial plants of the country, this study succeeded in producing transgenic cotton plants expressing Masp1 protein with increased fiber quality.

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