Heterologous expression of Chlorophytum borivilianum Squalene epoxidase in tobacco modulates stigmasterol production and alters vegetative and reproductive growth

Kaushal, Nishant; Verma, Deepika; Alok, Anshu; Pandey, Ashutosh; Singh, Kashmir

doi:10.1007/s00299-023-03000-1

Plant Cell Rep

2023

DOI: 10.1007/s00299-023-03000-1

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Heterologous expression of Chlorophytum borivilianum Squalene epoxidase in tobacco modulates stigmasterol production and alters vegetative and reproductive growth

Nishant Kaushal

Deepika Verma

Anshu Alok

et al.

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Cited by 2 publications

References 52 publications

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Integrated transcriptomic and metabolomic analysis reveals the molecular basis of tissue-specific accumulation of bioactive steroidal alkaloids in Fritillaria unibracteata

Liao,

Quan,

Huang

et al. 2023

Phytochemistry

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Integrated transcriptomic and metabolomic analysis reveals the molecular basis of tissue-specific accumulation of bioactive steroidal alkaloids in Fritillaria unibracteata

Liao,

Quan,

Huang

et al. 2023

Phytochemistry

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Light Regulated CoWRKY15 Acts on CoSQS Promoter to Promote Squalene Synthesis in Camellia oleifera Seeds

Li,

Du,

Zeng

et al. 2024

IJMS

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Squalene synthase (SQS) is the most direct key enzyme regulating squalene synthesis. To better understand the regulatory mechanisms of squalene biosynthesis, a 1423-bp long promoter region of the CoSQS gene was isolated from Camellia oleifera. Plant CARE and PLACE analysis affirmed the existence of the core promoter elements such as TATA and CAAT boxes and transcription factor binding sites like W-box and MYB in the isolated sequence. Exogenous factors regulating the CoSQS promoter were obtained by using Yeast one-hybrid screening, and the key transcription factor CoWRKY15 was found. AOS (Antibody Optimization System) analysis showed that CoWRKY15 had the highest interactions with a confidence level of 0.9026. Bioinformatics analysis showed that CoWRKY15 belonged to class 2 of the WRKY gene family. The results of subcellular localization showed that CoWRKY15 functioned in the nucleus. The results of CoWRKY15 promoter analysis showed that 8 out of 14 cis-elements with annotatable functions were related to the light response. The region of the CoSQS promoter that interacts with CoWRKY15 is −186 bp~−536 bp. The histochemical assay and squalene content suggested that the CoSQS promoter could drive the expression of GUS gene and specific promotion of CoSQS expression. It was found that CoWRKY15 could act on the −186 bp~−536 bp CoSQS promoter to regulate the expression of CoSQS and the content of squalene in C. oleifera seed kernels.

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Heterologous expression of Chlorophytum borivilianum Squalene epoxidase in tobacco modulates stigmasterol production and alters vegetative and reproductive growth

Cited by 2 publications

References 52 publications

Integrated transcriptomic and metabolomic analysis reveals the molecular basis of tissue-specific accumulation of bioactive steroidal alkaloids in Fritillaria unibracteata

Integrated transcriptomic and metabolomic analysis reveals the molecular basis of tissue-specific accumulation of bioactive steroidal alkaloids in Fritillaria unibracteata

Light Regulated CoWRKY15 Acts on CoSQS Promoter to Promote Squalene Synthesis in Camellia oleifera Seeds

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