Heterologous Expression of Genes Mediating Enhanced Fungal Resistance in Transgenic Wheat

Oldach, Klaus; Becker, Dirk; Lörz, Horst

doi:10.1094/mpmi.2001.14.7.832

Cited by 137 publications

(77 citation statements)

References 37 publications

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“…The transformation efficiency obtained in our study (2%) was similar to that observed in wheat by Vasil et al (1993), Oldach et al (2001) and MacKintosh et al (2006). However this efficiency might be considered a limitation if the study of many transgenes is tackled with limited operational capacity (Dahleen et al 2001).…”

Section: Discussionsupporting

confidence: 80%

Increased tolerance to wheat powdery mildew by heterologous constitutive expression of the Solanum chacoense Snakin-1 gene

Faccio¹,

Rovere²,

Hopp³

et al. 2011

CZECH J GENET PLANT

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Great efforts are currently being devoted to studying the use of transgenes to confer resistance to phytopathogenic fungi. Snakin-1 is a broad-spectrum antimicrobial peptide isolated from Solanum that is active in vitro against bacteria and fungi. Recently, it was reported that overexpression of the snakin-1 (SN1) gene in transgenic potato plants enhanced resistance to Rhizoctonia solani and Erwinia carotovora. In this work wheat transgenic plants that constitutively expressed the S. chacoense SN1 gene were challenged with Blumeria graminis f.sp. tritici. Enhanced resistance to the pathogen was observed in two transgenic lines in which the development of the disease was delayed and reduced compared with the wild type variety ProINTA Federal. An association between high resistance to the pathogen and a high level of snakin-1 transcripts in the plant was observed. This is the first report on SN1 gene expression in Gramineae and its effects on wheat powdery mildew development.

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Section: Discussionsupporting

confidence: 80%

Increased tolerance to wheat powdery mildew by heterologous constitutive expression of the Solanum chacoense Snakin-1 gene

Faccio¹,

Rovere²,

Hopp³

et al. 2011

CZECH J GENET PLANT

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“…This high level of basal non-specific resistance was found to be associated with the upregulation of two chitinases, three b-1,3-glucanases and four osmotins. Transgenic plants overexpressing chitinase alone or in combination with b-1,3-glucanase have been previously shown to display an enhanced resistance when challenged with a powdery mildew infection (Oldach et al, 2001) or bacterial pathogens (Dana et al, 2006). Delayed symptoms of Fusarium head blight have also been reported in these transgenic plants (Anand et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Diverse responses of wild and cultivated tomato to BABA, oligandrin andOidium neolycopersiciinfection

Satková

Starý

Plešková

et al. 2016

Ann Bot

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Background and Aims Current strategies for increased crop protection of susceptible tomato plants against pathogen infections include treatment with synthetic chemicals, application of natural pathogen-derived compounds or transfer of resistance genes from wild tomato species within breeding programmes. In this study, a series of 45 genes potentially involved in defence mechanisms was retrieved from the genome sequence of inbred reference tomato cultivar Solanum lycopersicum 'Heinz 1706'. The aim of the study was to analyse expression of these selected genes in wild and cultivated tomato plants contrasting in resistance to the biotrophic pathogen Oidium neolycopersici, the causative agent of powdery mildew. Plants were treated either solely with potential resistance inducers or by inducers together with the pathogen.Methods The resistance against O. neolycopersici infection as well as RT-PCR-based analysis of gene expression in response to the oomycete elicitor oligandrin and chemical agent b-aminobutyric acid (BABA) were investigated in the highly susceptible domesticated inbred genotype Solanum lycopersicum 'Amateur' and resistant wild genotype Solanum habrochaites.Key Results Differences in basal expression levels of defensins, germins, b-1,3-glucanases, heveins, chitinases, osmotins and PR1 proteins in non-infected and non-elicited plants were observed between the highly resistant and susceptible genotypes. Moreover, these defence genes showed an extensive up-regulation following O. neolycopersici infection in both genotypes. Application of BABA and elicitin induced expression of multiple defence-related transcripts and, through different mechanisms, enhanced resistance against powdery mildew in the susceptible tomato genotype.Conclusions The results indicate that non-specific resistance in the resistant genotype S. habrochaites resulted from high basal levels of transcripts with proven roles in defence processes. In the susceptible genotype S. lycopersicum 'Amateur', oligandrin-and BABA-induced resistance involved different signalling pathways, with BABAtreated leaves displaying direct activation of the ethylene-dependent signalling pathway, in contrast to previously reported jasmonic acid-mediated signalling for elicitins.

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“…Unfortunately, such promoters are typically dysfunctional in cereal species. Thus, expression of transgenes in cereals has been largely driven by ubiquitous promoters, such as those from the maize (Zea mays) ubiquitin 1 (Ubi1; Christensen et al, 1992;Oldach et al, 2001) or the rice (Oryza sativa) actin 1 gene (Act1; McElroy et al, 1990;Vickers et al, 2006). However, a few specific promoters derived from cereal species have been characterized and used to drive transgene expression.…”

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confidence: 99%

A Set of Modular Binary Vectors for Transformation of Cereals

et al. 2007

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Genetic transformation of crop plants offers the possibility of testing hypotheses about the function of individual genes as well as the exploitation of transgenes for targeted trait improvement. However, in most cereals, this option has long been compromised by tedious and low-efficiency transformation protocols, as well as by the lack of versatile vector systems. After having adopted and further improved the protocols for Agrobacterium-mediated stable transformation of barley (Hordeum vulgare) and wheat (Triticum aestivum), we now present a versatile set of binary vectors for transgene overexpression, as well as for gene silencing by double-stranded RNA interference. The vector set is offered with a series of functionally validated promoters and allows for rapid integration of the desired genes or gene fragments by GATEWAY-based recombination. Additional in-built flexibility lies in the choice of plant selectable markers, cassette orientation, and simple integration of further promoters to drive specific expression of genes of interest. Functionality of the cereal vector set has been demonstrated by transient as well as stable transformation experiments for transgene overexpression, as well as for targeted gene silencing in barley.

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Heterologous Expression of Genes Mediating Enhanced Fungal Resistance in Transgenic Wheat

Cited by 137 publications

References 37 publications

Increased tolerance to wheat powdery mildew by heterologous constitutive expression of the Solanum chacoense Snakin-1 gene

Increased tolerance to wheat powdery mildew by heterologous constitutive expression of the Solanum chacoense Snakin-1 gene

Diverse responses of wild and cultivated tomato to BABA, oligandrin andOidium neolycopersiciinfection

A Set of Modular Binary Vectors for Transformation of Cereals

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