Heterologous expression of the human polybromo-1 protein in the methylotrophic yeast Pichia pastoris

Hopson, Sarah; Thompson, Mark

doi:10.1016/j.pep.2018.07.002

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…It is well-known that Pichia pastoris protein expression system offers various advantages [19] including the success in high level production of a variety of heterologous proteins both intra-and extracellularly [20][21][22]. However, Pichia pastoris is a eukaryotic expression system, of which gene expression regulation system and material metabolic system were much more complex than prokaryotes expression systems, and the expression of some heterogeneous proteins in Pichia pastoris is unsatisfactory [11,22].…”

Section: Introductionmentioning

confidence: 99%

Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone

Deng

et al. 2020

Microb Cell Fact

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Porcine growth hormone (pGH) is a class of peptide hormones secreted from the pituitary gland, which can significantly improve growth and feed utilization of pigs. However, it is unstable and volatile in vitro. It needs to be encapsulated in liposomes when feeding livestock, whose high cost greatly limits its application in pig industry. Therefore we attempted to express pGH as intracellular soluble protein in Pichia pastoris and feed these yeasts with partial wall-breaking for swine, which could release directly pGH in intestine tract in case of being degraded in intestinal tract with low cost. In order to improve the intracellular soluble expression of pGH protein in Pichia pastoris and stability in vitro, we optimized the pGH gene, and screened molecular chaperones from E. coli and Pichia pastoris respectively for co-expressing with pGH. In addition, we had also explored conditions of mechanical crushing and fermentation. The results showed that the expression of intracellular soluble pGH protein was significantly increased after gene optimized and co-expressed with Ssa1-Sis1 chaperone from Pichia pastoris. Meanwhile, the optimal conditions of partial wall-breaking and fermentation of Pichia pastoris were confirmed, the data showed that the intracellular expression of the optimized pGH protein coexpressed with Ssa1-Sis1 could reach 340 mg/L with optimal conditions of partial wall-breaking and fermentation. Animal experiments verified that the optimized pGH protein co-expression with Ssa1-Sis1 had the best promoting effects on the growth of piglets. Our study demonstrated that Ssa1-Sis1 could enhance the intracellular soluble expression of pGH protein in Pichia pastoris and that partial wall-breaking of yeast could prevent pGH from degradation in vitro, release targetedly in the intestine and play its biological function effectively. Our study could provide a new idea to cut the cost effectively, establishing a theoretical basis for the clinic application of unstable substances in vitro.

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Section: Introductionmentioning

confidence: 99%

Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone

Deng

et al. 2020

Microb Cell Fact

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“…They 2 concluded that larger, preferably prospective, studies were needed to confirm and update their findings. Recently, in 2023, Demirel and Dilek 3 published, a study entitled “Relationship between body composition and PBRM1 mutations in clear cell renal cell carcinoma: a propensity score matching analysis” in the Journal of the Brazilian Medical Association 3 , in which they retrospectively examined the relationship between body muscle and adipose tissue composition in clear cell renal cell carcinoma patients with polybromo-1 protein (PBRM1) 4 gene mutation 3 . The study 3 concluded that normal attenuation muscle area is greater in patients with PBMR1 mutation, even after propensity score matching 3 .…”

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confidence: 99%

Comments on “Relationship between body composition and PBRM1 mutations in clear cell renal cell carcinoma: a propensity score matching analysis”

Pontes-Silva,

Kovaleva,

Gadzhiakhmedova

et al. 2023

Rev. Assoc. Med. Bras.

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The Draft Genome Sequence of Methylophilus sp. D22, Capable of Growing Under High Concentration of Methanol

Dai

Guo

et al. 2019

Curr Microbiol

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Heterologous expression of the human polybromo-1 protein in the methylotrophic yeast Pichia pastoris

Cited by 3 publications

References 24 publications

Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone

Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone

Comments on “Relationship between body composition and PBRM1 mutations in clear cell renal cell carcinoma: a propensity score matching analysis”

The Draft Genome Sequence of Methylophilus sp. D22, Capable of Growing Under High Concentration of Methanol

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