Heterologous Expression of Three Transcription Factors Differently Regulated Astragalosides Metabolic Biosynthesis in Astragalus membranaceus Hairy Roots

Li, Xiao Hua; Kim, Jae Kwang; Park, Sang Un

doi:10.3390/plants11141897

Cited by 10 publications

(3 citation statements)

References 68 publications

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“…Exogenous transcription factors also have a regulatory effect on the synthesis of astragalosides. Li et al (2022) [41] found that Arabidopsis MYB12, anthocyanin pigment 1 (PAP1), and maize leaf color (LC) transcription factors had regulatory effects on the synthesis of astragaloside metabolites. The overexpression of LC led to the accumulation of astragaloside I-IV.…”

Section: Astragalus Adventitious Roots Calycosin Isoflavone Glycosidementioning

confidence: 99%

Advances in Biotechnological Production and Metabolic Regulation of Astragalus membranaceus

Xuan

Zhang

et al. 2023

Plants

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Legume medicinal plants Astragalus membranaceus are widely used in the world and have very important economic value, ecological value, medicinal value, and ornamental value. The bioengineering technology of medicinal plants is used in the protection of endangered species, the rapid propagation of important resources, detoxification, and the improvement of degraded germplasm. Using bioengineering technology can effectively increase the content of secondary metabolites in A. membranaceus and improve the probability of solving the problem of medicinal plant resource shortage. In this review, we focused on biotechnological research into A. membranaceus, such as the latest advances in tissue culture, including callus, adventitious roots, hairy roots, suspension cells, etc., the metabolic regulation of chemical compounds in A. membranaceus, and the research progress on the synthetic biology of astragalosides, including the biosynthesis pathway of astragalosides, microbial transformation of astragalosides, and metabolic engineering of astragalosides. The review also looks forward to the new development trend of medicinal plant biotechnology, hoping to provide a broader development prospect for the in-depth study of medicinal plants.

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Section: Astragalus Adventitious Roots Calycosin Isoflavone Glycosidementioning

confidence: 99%

Advances in Biotechnological Production and Metabolic Regulation of Astragalus membranaceus

Xuan

Zhang

et al. 2023

Plants

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“…It resulted in a three-fold increase in camptothecin accumulation in Ophiorrhiza pumila hairy roots ( Hao et al., 2021 ). In another study, astragalosides production was increased in Astragalus membranaceus hairy roots by overexpressing Arabidopsis transcription factor MYB12, Production of anthocyanin pigment 1 (PAP1), and maize leaf color (Lc) transcription factors ( Li et al., 2022 ).…”

Section: Heterologous Expression Of Secondary Metabolites In Plantsmentioning

confidence: 99%

Plant-based expression platforms to produce high-value metabolites and proteins

Kulshreshtha¹,

Sharma

Padilla³

et al. 2022

Front. Plant Sci.

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Plant-based heterologous expression systems can be leveraged to produce high-value therapeutics, industrially important proteins, metabolites, and bioproducts. The production can be scaled up, free from pathogen contamination, and offer post-translational modifications to synthesize complex proteins. With advancements in molecular techniques, transgenics, CRISPR/Cas9 system, plant cell, tissue, and organ culture, significant progress has been made to increase the expression of recombinant proteins and important metabolites in plants. Methods are also available to stabilize RNA transcripts, optimize protein translation, engineer proteins for their stability, and target proteins to subcellular locations best suited for their accumulation. This mini-review focuses on recent advancements to enhance the production of high-value metabolites and proteins necessary for therapeutic applications using plants as bio-factories.

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“…Thus, elicitation with biotic or abiotic elicitors is believed to increase phytochemicals biosynthesis in PTC-based approaches. The use of methyl jasmonate (MJ) and salicylic acid (SA) as abiotic elicitors enhances metabolite biosynthesis in various medicinal plants (Halder et Astragalosides are one of the major groups of active compounds in A. membranaceus (Li et al 2022). Astragaloside IV has been recognized as the main component of the aqueous extract of Astragalus.…”

Section: Introductionmentioning

confidence: 99%

In vitro micropropagation and hairy root induction for astragaloside IV production in Astragalus membranaceus var. mongholicus

Taghizadeh,

Özzambak,

Zeybekoglu

et al. 2023

Preprint

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Astragalus membranaceus var. mongholicus, is an important natural source of bioactive constituents. Pharmacological and clinical studies of secondary metabolites have confirmed that A. membranaceus has diverse biological functions. Plant tissue culture-based techniques and hairy root and adventitious root cultures are often used as practical platforms for the exploitation of biochemical compounds from medicinal plants. In this study, micropropagation of A. membranaceus was optimized for rapid proliferation, and hairy roots were induced by three strains of Agrobacterium rhizogenes (A4, A13, and MSU), on two leaf explant ages (10- and 20-day old), and three inoculation times (10, 20, and 30 min). In addition, the effects of three concentrations (100, 250, and 500 µM L− 1) of salicylic acid (SA) and methyl jasmonate (MJ) on astragaloside IV accumulation in A. membranaceus hairy roots were investigated. The highest number and length of branches were obtained on MS medium supplemented with 1 mg L− 1 BAP. Strain A13 caused the highest rate of induction of hairy root in leaf explants. In addition, since the age of explants had a significant effect on hairy root formation, the youngest explants obtained the highest number and longest hairy roots. Results showed that inducers of methyl jasmonate and salicylic acid elicitors could effectively stimulate the content of astragaloside. Our study showed that treating hairy root cultures with 250 µM MJ or 500 µM SA effectively increased astragaloside-IV accumulation in A. membranaceus hairy roots. Therefore, The findings provide valuable insights into optimal conditions for increasing large-scale production of astragaloside-IV such as industrial production in bioreactors.

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Heterologous Expression of Three Transcription Factors Differently Regulated Astragalosides Metabolic Biosynthesis in Astragalus membranaceus Hairy Roots

Cited by 10 publications

References 68 publications

Advances in Biotechnological Production and Metabolic Regulation of Astragalus membranaceus

Advances in Biotechnological Production and Metabolic Regulation of Astragalus membranaceus

Plant-based expression platforms to produce high-value metabolites and proteins

In vitro micropropagation and hairy root induction for astragaloside IV production in Astragalus membranaceus var. mongholicus

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