Heterologous expression of two <i>Arabidopsis</i> starch dikinases in potato

Xuan, Xuenan; Dees, Dianka C.T.; Huang, Xing; Visser, Richard G. F.; Trindade, Luisa M.

doi:10.1002/star.201600324

Cited by 4 publications

(4 citation statements)

References 24 publications

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“…Heterologous expression of Arabidopsis thaliana GWD2 and GWD3 in potato tubers would alter starch granule morphology, but not change the starch phosphate content (Xu et al, 2018).…”

Section: Gwdmentioning

confidence: 97%

“…Additionally, suppressed GWD expression in transgenic potato lines would decrease PV, BD, and CPV, but had no significant effects on starch gelatinization (Wickramasinghe et al., 2009). Heterologous expression of Arabidopsis thaliana GWD2 and GWD3 in potato tubers would alter starch granule morphology, but not change the starch phosphate content (Xu et al., 2018).…”

Section: Biosynthesis Of Potato Starchmentioning

confidence: 99%

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Toward an understanding of potato starch structure, function, biosynthesis, and applications

Tong

Chen

et al. 2023

Food Frontiers

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Starch is the most important component in potato tubers, whose structure and composition play key roles in properties of potato storage, processing, and applications. This review identifies the biodiversity of starch content, structure, and physiochemical properties, particularly the amylose and amylopectin content, molecular and granular structure, gelatinization, retrogradation, swelling power, solubility, and digestibility of potato in the past decade. Various functions of major potato starch biosynthesis‐related enzymes, such as AGPase, starch synthase, starch branching enzyme, debranching enzyme, and glucan/water dikinase, are also summarized. The potential utilization of native and modified potato starch in food and medicine fields owing to its high pasting viscosity is also analyzed. This review will contribute to a better understanding of the genetics–structure–functions of potato starch and may provide insights into potential potato starch utilization and further genetic improvement of starch quality.

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“…Heterologous expression of Arabidopsis thaliana GWD2 and GWD3 in potato tubers would alter starch granule morphology, but not change the starch phosphate content (Xu et al, 2018).…”

Section: Gwdmentioning

confidence: 97%

Section: Biosynthesis Of Potato Starchmentioning

confidence: 99%

Toward an understanding of potato starch structure, function, biosynthesis, and applications

Tong

Chen

et al. 2023

Food Frontiers

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“…A third homologue of the potato GWD was identified in Arabidopsis thaliana by screening the Arabidopsis genome and named AtGWD3 . Xu et al introduced two starch dikinases ( AtGWD2 and AtGWD3 ) from Arabidopsis into two different potato cultivars and found that there was no significant increase in starch phosphate content, but modified starches showed changes in granule morphology. It was also found that sucrose synthase 4 (Susy4) was up‐regulated in transgenic tubers with AtGWD2 expression …”

Section: Transgenic Engineeringmentioning

confidence: 99%

Improving Starch‐Related Traits in Potato Crops: Achievements and Future Challenges

et al. 2018

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Potato is one of the most important starch crops in the world and is used as a staple food for more than one billion people worldwide. Starch is the main storage compound in potato tubers and is of considerable value in food and non-food applications. The starch biosynthesis pathway is the principal biochemical pathway in plants. Potato starch bioengineering can be considered complicated and has been an extensive subject of study over the past three decades. Tetraploid inheritance is the most conspicuous feature linked with potato breeding. Genetic map construction and linkage mapping have contributed greatly to potato breeding, and association studies have further enhanced our knowledge to carry out optimized and comprehensive breeding programes. This review begins with the introduction of the starch structure with respect to functionality, the starch biosynthesis pathway and related genes/enzymes participating in the pathway. Then, the advances that have been made during the past years in the study of genetic basis of starchrelated traits by linkage and association mapping are summarized. Molecular breeding via transgenic engineering of starch biosynthesis-related genes to modify starch properties is also reviewed. Finally, future challenges and new directions for improving starch quality in potato and the implication of new genome editing tools to produce the next generation starches are proposed. A better understanding of these factors is necessary to conduct molecular breeding programes in potato to improve starch quality.

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“…This could be achieved through plant breeding program and/or biotechnological approach. For instance, in order to alter the amount of negative charge of starch granules, plant scientists can add or remove starch-attached phosphate groups (charged negatively) by manipulating the expression of key genes involved in starch biosynthesis (Lanahan and Basu, 2006; Schewe et al., 2007; Carciofi et al., 2011; Frohberg, 2012; Xu et al., 2017a,b, 2018). We firmly believe that such design can be realized through joint efforts from plant scientists and biorefinery scientist and will play an important role in elevating the starch biorefinery to new heights.…”

Section: Creating Novel Biorefinery Concepts Through a Cross-disciplimentioning

confidence: 99%

Exploring the Treasure of Plant Molecules With Integrated Biorefineries

et al. 2019

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Despite significant progress toward the commercialization of biobased products, today’s biorefineries are far from achieving their intended goal of total biomass valorization and effective product diversification. The problem is conceptual. Modern biorefineries were built around well-optimized, cost-effective chemical synthesis routes, like those used in petroleum refineries for the synthesis of fuels, plastics, and solvents. However, these were designed for the conversion of fossil resources and are far from optimal for the processing of biomass, which has unique chemical characteristics. Accordingly, existing biomass commodities were never intended for modern biorefineries as they were bred to meet the needs of conventional agriculture. In this perspective paper , we propose a new path toward the design of efficient biorefineries, which capitalizes on a cross-disciplinary synergy between plant, physical, and catalysis science. In our view, the best opportunity to advance profitable and sustainable biorefineries requires the parallel development of novel feedstocks, conversion protocols and synthesis routes specifically tailored for total biomass valorization. Above all, we believe that plant biologists and process technologists can jointly explore the natural diversity of plants to synchronously develop both, biobased crops with designer chemistries and compatible conversion protocols that enable maximal biomass valorization with minimum input utilization. By building biorefineries from the bottom-up (i.e., starting with the crop), the envisioned partnership promises to develop cost-effective, biomass-dedicated routes which can be effectively scaled-up to deliver profitable and resource-use efficient biorefineries.

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Heterologous expression of two Arabidopsis starch dikinases in potato

Cited by 4 publications

References 24 publications

Toward an understanding of potato starch structure, function, biosynthesis, and applications

Toward an understanding of potato starch structure, function, biosynthesis, and applications

Improving Starch‐Related Traits in Potato Crops: Achievements and Future Challenges

Exploring the Treasure of Plant Molecules With Integrated Biorefineries

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