Heterologous expression of xylose specific transporter improves xylose utilization by recombinant Zymomonas mobilis strain in presence of glucose

Sarkar, Payel; Goswami, Gargi; Mukherjee, Mayurketan; Das, Debasish

doi:10.1016/j.procbio.2021.01.006

Cited by 12 publications

(8 citation statements)

References 25 publications

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“…The Glf has a high affinity for glucose, causing competitive inhibition of xylose uptake in mixed sugar supplemented medium [ 89 ]. Recently, some xylose-specific transporters, that is, XylE, and ABC type transporter, were successfully introduced in Z. mobilis to enhance the rate and extent of xylose uptake [ 90 , 91 ]. An experiment demonstrated , recently, that overexpression of ABC type transporter enhances the rate of xylose utilization by 48.9% as compared to parental stains in the presence of glucose [ 91 ].…”

Section: Recent Metabolic Advances In Microorganism For Biofuel Produ...mentioning

confidence: 99%

“…Recently, some xylose-specific transporters, that is, XylE, and ABC type transporter, were successfully introduced in Z. mobilis to enhance the rate and extent of xylose uptake [ 90 , 91 ]. An experiment demonstrated , recently, that overexpression of ABC type transporter enhances the rate of xylose utilization by 48.9% as compared to parental stains in the presence of glucose [ 91 ]. Recent efforts in Z. mobilis engineering have also enabled several strains to tolerate furfural and acetic acids, which are the predominant toxic inhibitors during lignocellulosic ethanol production [ 92 , 93 ].…”

Section: Recent Metabolic Advances In Microorganism For Biofuel Produ...mentioning

confidence: 99%

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Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels

et al. 2022

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Combating climate change and ensuring energy supply to a rapidly growing global population has highlighted the need to replace petroleum fuels with clean, and sustainable renewable fuels. Biofuels offer a solution to safeguard energy security with reduced ecological footprint and process economics. Over the past years, lignocellulosic biomass has become the most preferred raw material for the production of biofuels, such as fuel, alcohol, biodiesel, and biohydrogen. However, the cost-effective conversion of lignocellulose into biofuels remains an unsolved challenge at the industrial scale. Recently, intensive efforts have been made in lignocellulose feedstock and microbial engineering to address this problem. By improving the biological pathways leading to the polysaccharide, lignin, and lipid biosynthesis, limited success has been achieved, and still needs to improve sustainable biofuel production. Impressive success is being achieved by the retouring metabolic pathways of different microbial hosts. Several robust phenotypes, mostly from bacteria and yeast domains, have been successfully constructed with improved substrate spectrum, product yield and sturdiness against hydrolysate toxins. Cyanobacteria is also being explored for metabolic advancement in recent years, however, it also remained underdeveloped to generate commercialized biofuels. The bacterium Escherichia coli and yeast Saccharomyces cerevisiae strains are also being engineered to have cell surfaces displaying hydrolytic enzymes, which holds much promise for near-term scale-up and biorefinery use. Looking forward, future advances to achieve economically feasible production of lignocellulosic-based biofuels with special focus on designing more efficient metabolic pathways coupled with screening, and engineering of novel enzymes.

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Section: Recent Metabolic Advances In Microorganism For Biofuel Produ...mentioning

confidence: 99%

Section: Recent Metabolic Advances In Microorganism For Biofuel Produ...mentioning

confidence: 99%

Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels

et al. 2022

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“…Although xylose can be transported across the membrane, the affinity is 200 times lower than that of glucose ( Kawaguchi et al, 2006 ; Meinander and Hahn-Hagerdal, 1997 ). Similarly, xylose is transported through glucose-facilitated diffusion protein in Z. mobilis , which also limits the transmembrane transport of xylose ( Sarkar et al, 2021 ). As the most common microorganism for lignocellulosic hydrolysates fermentation, much effort has been made to increase xylose transmembrane transport and co-utilization in S. cerevisiae .…”

Section: Microbial Metabolic Engineering For Enhanced Utilization Of ...mentioning

confidence: 99%

“…Erbeznik et al analyzed the xylFGH operon and xylose ABC transporter in Thermoanaerobacter ethanolicus ( Erbeznik et al, 2004 ). Though introducing a xylose transporter (ABC type transporter system) into Z. mobilis , xylose utilization of which was increased by 48.9%, and the fermentation time was greatly shortened ( Sarkar et al, 2021 ). The xylose/arabinose transport in Sulfolobus acidocaldarius via a 2 (CUT2)-Type ABC transporter, which is the first CUT2 family ABC transporter analyzed in the domain of Archaea ( Wagner et al, 2018 ).…”

Section: Microbial Metabolic Engineering For Enhanced Utilization Of ...mentioning

confidence: 99%

Biosynthesis of value-added bioproducts from hemicellulose of biomass through microbial metabolic engineering

Geng

Jia

Peng

et al. 2022

Metabolic Engineering Communications

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“…However, most microbes are only able to use glucose as an energy source, and those that can utilize xylose generally suppress its metabolic processing when glucose is available through a process known as carbon catabolite repression [ 8–10 ]. Such repression enables microbes to preferentially metabolize carbon sources that are more easily processed by suppressing the expression and activity of enzymes associated with the processing of non-preferred carbon sources [ 11 , 12 ]. The identification of oleaginous yeasts capable of simultaneously utilizing both xylose and glucose has been an active area of research interest, with several such yeast strains having been reported to date including Trichosporon cutaneum (present name, Cutaneotrichosporon cutaneum ) and Cystobasidium iriomotense [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Draft Genome Assembly and Annotation for Cutaneotrichosporon dermatis NICC30027, an Oleaginous Yeast Capable of Simultaneous Glucose and Xylose Assimilation

et al. 2022

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The identification of oleaginous yeast species capable of simultaneously utilizing xylose and glucose as substrates to generate value-added biological products is an area of key economic interest. We have previously demonstrated that the Cutaneotrichosporon dermatis NICC30027 yeast strain is capable of simultaneously assimilating both xylose and glucose, resulting in considerable lipid accumulation. However, as no high-quality genome sequencing data or associated annotations for this strain are available at present, it remains challenging to study the metabolic mechanisms underlying this phenotype. Herein, we report a 39,305,439 bp draft genome assembly for C. dermatis NICC30027 comprised of 37 scaffolds, with 60.15% GC content. Within this genome, we identified 524 tRNAs, 142 sRNAs, 53 miRNAs, 28 snRNAs, and eight rRNA clusters. Moreover, repeat sequences totaling 1,032,129 bp in length were identified (2.63% of the genome), as were 14,238 unigenes that were 1,789.35 bp in length on average (64.82% of the genome). The NCBI non-redundant protein sequences (NR) database was employed to successfully annotate 11,795 of these unigenes, while 3,621 and 11,902 were annotated with the Swiss-Prot and TrEMBL databases, respectively. Unigenes were additionally subjected to pathway enrichment analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), Clusters of orthologous groups for eukaryotic complete genomes (KOG), and Non-supervised Orthologous Groups (eggNOG) databases. Together, these results provide a foundation for future studies aimed at clarifying the mechanistic basis for the ability of C. dermatis NICC30027 to simultaneously utilize glucose and xylose to synthesize lipids.

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Heterologous expression of xylose specific transporter improves xylose utilization by recombinant Zymomonas mobilis strain in presence of glucose

Cited by 12 publications

References 25 publications

Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels

Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels

Biosynthesis of value-added bioproducts from hemicellulose of biomass through microbial metabolic engineering

Draft Genome Assembly and Annotation for Cutaneotrichosporon dermatis NICC30027, an Oleaginous Yeast Capable of Simultaneous Glucose and Xylose Assimilation

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