1992
DOI: 10.1016/0378-1119(92)90043-o
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Heterologous gene expression in Aspergillus niger: a glucoamylase-porcine pancreatic prophospholipase A2 fusion protein is secreted and processed to yield mature enzyme

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Cited by 67 publications
(44 citation statements)
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“…Previous work in this lab showed that protease degradation contributed the most to the GFP loss [10]. Other researchers have also shown that proteases are responsible for the degradation of heterologous proteins such as human interleukin-6 [5], bovine chymosin [18] and porcine pancreatic phospholipase A [14]. Since protease activity is strongly affected by pH values, the recombinant protein production can increase if broth pH is properly controlled at a value at which protease activity is low, e.g., pH 5 or 6.…”
Section: Effect Of Ph On Protease Activitymentioning
confidence: 99%
“…Previous work in this lab showed that protease degradation contributed the most to the GFP loss [10]. Other researchers have also shown that proteases are responsible for the degradation of heterologous proteins such as human interleukin-6 [5], bovine chymosin [18] and porcine pancreatic phospholipase A [14]. Since protease activity is strongly affected by pH values, the recombinant protein production can increase if broth pH is properly controlled at a value at which protease activity is low, e.g., pH 5 or 6.…”
Section: Effect Of Ph On Protease Activitymentioning
confidence: 99%
“…Large-scale fermentation technology for filamentous fungi is well established and several species have U.S. Food and Drug Administration approval for use in the food industry ("Generally Regarded as Safe" status). There is a great interest in the production of heterologous proteins in filamentous fungi (Fowler and Berka, 1991;Gouka et al, 1996;Jeenes et al, 1994;Roberts et al, 1992;van den Hondel et al, 1991). In general, the secreted yields of most heterologous proteins are low when compared with the homologous proteins, and reach levels that in most cases do not exceed a few milligrams per liter of culture medium.…”
Section: Introductionmentioning
confidence: 98%
“…This improvement factor is less than that obtained for homologous production of FAEA in A. niger (34). Apparently, as seen for other fusion proteins, the production yield was negatively affected by the presence of the bacterial domain (21,31,35,36). In addition, we observed that FAEADoc protein was produced as a truncated form.…”
Section: Discussionmentioning
confidence: 52%
“…In earlier studies, methods were developed to alleviate limitations for protein production in fungal hosts. An efficient strategy is based on the use of translational fusions in which the targeted protein is fused to an endogenous secreted carrier protein at the N-terminal end of the heterologous protein (17,35). The N-terminal carrier protein is believed to improve the secretion efficiency of the heterologous protein by facilitating translocation and folding in the endoplasmic reticulum.…”
Section: Discussionmentioning
confidence: 99%
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