Heterologous Prime-Boost Vaccination Enhances TsPmy’s Protective Immunity against Trichinella spiralis Infection in a Murine Model

Wang, Lei; Sun, Xiaotao; Huang, Jingjing; Zhan, Bin; Zhu, Xiaoyan

doi:10.3389/fmicb.2017.01394

Cited by 11 publications

(8 citation statements)

References 32 publications

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“…Vaccinated mice exhibited a 54% reduction in adult worm burden of intestines at day 5 after challenge and a 33% reduction in larval burden of muscles at day 35 post infection. The larval and adult burden reduction observed in our study is similar to those from previous studies (Cui et al 2013, Long et al 2014, Wang et al 2017b. Our results were also consistent with previous reports which indicated that Th2-predominant immune responses in vaccinated mice have been shown to be important, of which anti-Trichinella antibodies play an important role in immune protection against infection with T. spiralis (see Wang et al 2013b, Long et al 2014.…”

Section: A B D C E Fsupporting

confidence: 93%

Cloning, expression and characterisation of a cysteine protease from Trichinella spiralis

Song¹,

Wang²,

Ren

et al. 2018

FOLIA PARASIT

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Cysteine protease is a superfamily of widespread proteolytic enzymes and plays a major role in larval invasion, migration, exsheathing, survival and immune evasion in parasites. In the present study, the gene coding cysteine proteinase of the nematode Trichinella spiralis (Owen, 1835) was cloned into pQE-80L and subsequently expressed in E. coli JM109. The rTsCP was purified and its antigenicity was identified by Western blot and ELISA. Using anti-rTsCP serum the native TsCP was identified in muscle larval crude proteins. The results of quantitative real-time PCR and immunofluorescence test demonstrated that the TsCP was expressed in all stages of T. spiralis and located mainly in cuticle, stichosome and reproductive organs. The immunisation of mice with rTsCP elicited Th2-predominant immune responses. Anti-rTsCP antibodies could partially inhibit the in vitro larval invasion of intestinal epithelial cells and kill the newborn larvae by an antibody-dependent cell-mediated dose-dependent cytotoxicity. The vaccinated mice exhibited a 54% reduction of adults and a 33% reduction of muscle larvae following challenge infection. The results suggested that the TsCP might be an indispensable protein in Trichinella invasion, development and survival of T. spiralis in hosts, and could be a potential vaccine target against infection.

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Section: A B D C E Fsupporting

confidence: 93%

Cloning, expression and characterisation of a cysteine protease from Trichinella spiralis

Song¹,

Wang²,

Ren

et al. 2018

FOLIA PARASIT

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“…Therefore, in order to improve vaccination efficacy, the Trichinella common antigens in ES products and worm surface antigens from different worm stages are required to be used for development of anti- Trichinella vaccine, and the multivalent vaccines against various T. spiralis stages (especially the early IIL and NBL stages) should be explored [ 9 ]. Moreover, other vaccination strategies including the heterologous prime-boost immunization, genetic adjuvants or codon optimization, and different vaccination routes should also be developed [ 69 ]. For example, intranasal vaccination of mice with attenuated Salmonella expressing a T. spiralis gp43 antigen-derived 30-mer peptide fused with adjuvant C3d-P28 has achieved a 92.8% intestinal adult reduction following challenge infection [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Oral vaccination with Trichinella spiralis DNase II DNA vaccine delivered by attenuated Salmonella induces a protective immunity in BALB/c mice

Han

Jiang

et al. 2018

Vet Res

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Trichinellosis is one of the most serious foodborne parasitic zoonosis with worldwide distribution, and it is necessary to develop a vaccine to interrupt transmission from animals to humans. Trichinella spiralis adult-specific DNase II-1 (TsDNase II) were identified by immunoproteomics in surface or excretory/secretory proteins of adult worms (AW) and intestinal infective larvae (IIL). The aim of this study was to investigate the systemic, mucosal responses and immune protection elicited by oral vaccination with TsDNase II DNA vaccine delivered by attenuated Salmonella typhimurium strain⊿cyaSL1344. Oral vaccination with TsDNase II DNA vaccine triggered an obvious mucosal sIgA response and a systemic IgG response in mice, and IgG1 was predominant. Th1 (IFN-γ) and Th2 (IL-4, 10) cytokines were distinctly increased in the spleen and mesenteric lymph node (MLN) cells of vaccinated mice. An indirect immunofluorescent test revealed that native TsDNase II is present at the cuticle of this nematode after the 2nd molting, further confirming that TsDNase II is adult-specific and expressed at AW and pre-adult stages. Oral immunization of mice with TsDNase II exhibited a 53.85% reduction in AW and a 59.26% reduction in ML after larval challenge. The in vitro NBL production of adult females from TsDNase II-vaccinated mice was also reduced in comparison with pcDNA3.1 or the PBS control group (P < 0.01). Our results show that oral immunization of mice with TsDNase II produced an intestinal and systematic concurrent Th1/Th2 immune response, and a significant immune protection against challenge.Electronic supplementary materialThe online version of this article (10.1186/s13567-018-0614-y) contains supplementary material, which is available to authorized users.

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“…The monoclonal antibody, 9G3, which targets the complement binding site of Ts-Pmy, could partially block its complement inhibitory activity, thereby increasing complement-mediated killing of larvae ( Hao et al, 2014 ). Mice immunized with recombinant protein ( Yang et al, 2010 ), epitope peptides ( Wei et al, 2011 ; Gu et al, 2013 , 2017 ), or the DNA ( Wang et al, 2016 , 2017 ) of Ts-Pmy were conferred with significant protection against the challenge of T. spiralis muscle larvae. Thus, Ts-Pmy has become the leading vaccine candidate for the control of trichinellosis.…”

Section: Parasite-generated Complement Regulatory Proteins As Vaccinementioning

confidence: 99%

Complement Evasion: An Effective Strategy That Parasites Utilize to Survive in the Host

et al. 2019

Self Cite

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Parasitic infections induce host immune responses that eliminate the invading parasites. However, parasites have evolved to develop many strategies to evade host immune attacks and survive in a hostile environment. The complement system acts as the first line of immune defense to eliminate the invading parasites by forming the membrane attack complex (MAC) and promoting an inflammatory reaction on the surface of invading parasites. To date, the complement activation pathway has been precisely delineated; however, the manner in which parasites escape complement attack, as a survival strategy in the host, is not well understood. Increasing evidence has shown that parasites develop sophisticated strategies to escape complement-mediated killing, including (i) recruitment of host complement regulatory proteins on the surface of the parasites to inhibit complement activation; (ii) expression of orthologs of host RCA to inhibit complement activation; and (iii) expression of parasite-encoded proteins, specifically targeting different complement components, to inhibit complement function and formation of the MAC. In this review, we compiled information regarding parasitic abilities to escape host complement attack as a survival strategy in the hostile environment of the host and the mechanisms underlying complement evasion. Effective escape of host complement attack is a crucial step for the survival of parasites within the host. Therefore, those proteins expressed by parasites and involved in the regulation of the complement system have become important targets for the development of drugs and vaccines against parasitic infections.

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Heterologous Prime-Boost Vaccination Enhances TsPmy’s Protective Immunity against Trichinella spiralis Infection in a Murine Model

Cited by 11 publications

References 32 publications

Cloning, expression and characterisation of a cysteine protease from Trichinella spiralis

Cloning, expression and characterisation of a cysteine protease from Trichinella spiralis

Oral vaccination with Trichinella spiralis DNase II DNA vaccine delivered by attenuated Salmonella induces a protective immunity in BALB/c mice

Complement Evasion: An Effective Strategy That Parasites Utilize to Survive in the Host

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