Heterologous production of 3-hydroxyvalerate in engineered Escherichia coli

Miscevic, Dragan; Srirangan, Kajan; Kefale, Teshager; Kilpatrick, Shane; Chung, Duane A.; Moo‐Young, Murray; Chou, C. Perry

doi:10.1016/j.ymben.2019.11.005

Cited by 12 publications

(12 citation statements)

References 47 publications

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“…Similarly, optimal values of 30°C and pH 7 were observed by Masood et al (2012) for the specie Bacillus cereus. Moreover, PHBV production by engineered Escherichia coli at 30°C was found to be significantly higher than those at 37°C (Miscevic et al 2019).…”

Section: Operating Conditionsmentioning

confidence: 95%

“…Finally, metabolic engineering techniques have been tested and proposed, obtaining interesting results: Ralstonia eutropha, Haloferax Mediterranei and Alcaliges species have been modified to enhance their productivity (Choi et al 2003;Yoon et al 1995;Zhao et al 2013). Moreover, other species such as Escherichia coli, Halomonas, Aeromonas and Salmonella enterica, which do not produce biopolymers, have been engineered to produce PHBV (Aldor et al 2002;Wu et al 2012;Miscevic et al 2019;Shi et al 2020).…”

Section: Techno-economic Analysis and Production Strategiesmentioning

confidence: 99%

“…Other authors reported the derivation of engineered Escherichia coli strains for PHBV production from unrelated carbon sources. Miscevic et al (2019) enabled the intracellular formation of non-native propionyl-CoA and investigated various enzymes involved in 3HV biosynthetic pathway from different microorganisms. The engineered Escherichia coli strains produced PHBV from glucose and glycerol as sole carbon sources.…”

Section: Metabolic Engineering Strategiesmentioning

confidence: 99%

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Improving biological production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) co-polymer: a critical review

Policastro

Pánico

Fabbricino

2021

Rev Environ Sci Biotechnol

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Although poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is the most promising biopolymer for petroleum-based plastics replacement, the low processes productivity as well as the high sale price represent a major barrier for its widespread usage. The present work examines comparatively the existing methods to enhance the yield of the PHBV co-polymer biologically produced and/or reduce their costs. The study is addressed to researchers working on the development of new biological production methods and/or the improvement of those currently used. At this aim, the authors have considered the analysis of some crucial aspects related to substrates and microorganism’s choice. The production strategies have been individuated, presented and discussed, either based on a single aspect (type of substrate or microorganism) or based on combined aspects (type of substrate and microorganism). Process operating conditions have been discussed as well. The analysis indicates that the addition of 3HV precursors is capable to dramatically enhance the hydroxyvalerate fraction in the produced biopolymers. On the other hand, due to the high costs of the 3HV precursors, the utilization of wild bacterial species capable to produce the hydroxyvalerate fraction from unrelated carbon sources (i.e. no 3HV precursors) also can be considered a valuable strategy for costs reduction. Moreover, metabolic engineering techniques can be successfully used to promote 3HV precursors-independent biosynthesis pathways and enhance the process productivity. The use of mixed cultures or extremophile bacteria avoids the need of sterile working conditions, and therefore favours the process scale-up. The utilization of the organic waste as substrate plays a key role for a sharp reduction of production costs. Finally, the selection of the most suitable substrate-microorganism combination cannot be separated by the adoption of an appropriate choice of reactor configuration and abiotic factors. Graphic abstract

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Section: Operating Conditionsmentioning

confidence: 95%

Section: Techno-economic Analysis and Production Strategiesmentioning

confidence: 99%

Section: Metabolic Engineering Strategiesmentioning

confidence: 99%

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Improving biological production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) co-polymer: a critical review

Policastro

Pánico

Fabbricino

2021

Rev Environ Sci Biotechnol

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“…Recently, (R)-3-hydroxyvalerate (R3HVA) and R3HBA were produced in a recombinant strain of E. coli capable of glycerol conversion (Miscevic et al, 2019). The engineered strain (P3HA31) harbors the phaA and bktB genes (from Cupriavidus necator ATCC 43291) to catalyze the condensation of two acetyl-CoA molecules or an acetyl-CoA and a propionyl-CoA molecule for the formation of acetoacetyl-CoA and 3ketovaleryl-CoA, respectively.…”

Section: Production Of 3has In Recombinant Ecoli Strainsmentioning

confidence: 99%

“…Carbon substrate 3HA titer (gL −1 ) a 3HA Volumetric productivity (gL −1 h −1 ) References E. coli DH5α(phbA-phbB-buk+ ptb) Glucose 12.0 (R3HBA) 0.25 Gao et al, 2002 E. coli XLI-Blue (phaA-phaB-phaC-phaZi) Glucose 9.6 (R3HBA) 0.19 Lee and Lee, 2003 E. coli DH5 (phbA-phbB-phaG) Glucose + Acrylic acid 0.7 (R3HBA) 0.01 Zhao et al, 2003 E. coli JM 109 (pha CAB-phaZ7) Glucose 7.3 (R3HBA) 0.073 Shiraki et al, 2006 UV Glycerol as carbon and energy source, aerobic 2.0 (R3HBA) 0.042 Miscevic et al, 2019 3.71 (R3HVA) 0.077 a R3HBA, (R)-3-hydroxybutyric acid; R3HDD, (R)-3-hydroxydodecanoic acid; S3HBA, (S)-3-hydroxybutyric acid; S3HVA, (S)-3-hydroxyvaleric acid.…”

Section: Strainsmentioning

confidence: 99%

Beyond Intracellular Accumulation of Polyhydroxyalkanoates: Chiral Hydroxyalkanoic Acids and Polymer Secretion

Yáñez

Conejeros

Vergara-Fernández

et al. 2020

Front. Bioeng. Biotechnol.

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Polyhydroxyalkanoates (PHAs) are ubiquitous prokaryotic storage compounds of carbon and energy, acting as sinks for reducing power during periods of surplus of carbon source relative to other nutrients. With close to 150 different hydroxyalkanoate monomers identified, the structure and properties of these polyesters can be adjusted to serve applications ranging from food packaging to biomedical uses. Despite its versatility and the intensive research in the area over the last three decades, the market share of PHAs is still low. While considerable rich literature has accumulated concerning biochemical, physiological, and genetic aspects of PHAs intracellular accumulation, the costs of substrates and processing costs, including the extraction of the polymer accumulated in intracellular granules, still hampers a more widespread use of this family of polymers. This review presents a comprehensive survey and critical analysis of the process engineering and metabolic engineering strategies reported in literature aimed at the production of chiral (R)-hydroxycarboxylic acids (RHAs), either from the accumulated polymer or by bypassing the accumulation of PHAs using metabolically engineered bacteria, and the strategies developed to recover the accumulated polymer without using conventional downstream separations processes. Each of these topics, that have received less attention compared to PHAs accumulation, could potentially improve the economy of PHAs production and use. (R)-hydroxycarboxylic acids can be used as chiral precursors, thanks to its easily modifiable functional groups, and can be either produced de-novo or be obtained from recycled PHA products. On the other hand, efficient mechanisms of PHAs release from bacterial cells, including controlled cell lysis and PHA excretion, could reduce downstream costs and simplify the polymer recovery process.

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Screening method to prioritize relevant bio‐based acids and their biochemical processes using recent patent information

Braga

Ferreira

Almeida

2020

Biofuels Bioprod Bioref

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There are increasing environmental and economic demands for the production of chemicals of industrial interest from renewable resources. Among the renewable products, short‐chain carboxylic acids (C1‐C6) stand out due to their importance in many sectors of the economy. Within this class, a wide variety of acids are industrially valuable as the precursors of many other chemicals for different applications, technological solutions, and markets. This makes it difficult to identify the most promising from a technical and economic standpoint. In this work, data on scientific papers and patents published between 2008 to 2017, related to the main bio‐based carboxylic acids, are used to identify the acids that are generating growing scientific and industrial interest. The biochemical processes in patents associated with the production of such acids are evaluated using a multicriteria approach that integrates valuation patent criteria. The results of this study show that acids that are of substantial commercial interest, such as acetic, citric, lactic, acrylic, and methacrylic acids, have the highest growth rate both in scientific papers and patents. In terms of biochemical processes, the multicriteria decision analysis showed that the most relevant processes described in patents were associated with ‘drop‐in’ acids such as methacrylic, adipic, maleic, acrylic, and glutaric acids. There is also a clear indication of the emergence of studies on low volume acids such as acetoacetic, acetolactic, muconic, and levulinic acids. In conclusion, the analysis shows that emerging scientific and technological production is conservative, focusing on acids with established markets, especially for the substitution of petrochemicals for immediate demand. © 2020 Society of Industrial Chemistry and John Wiley & Sons Ltd

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Heterologous production of 3-hydroxyvalerate in engineered Escherichia coli

Cited by 12 publications

References 47 publications

Improving biological production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) co-polymer: a critical review

Improving biological production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) co-polymer: a critical review

Beyond Intracellular Accumulation of Polyhydroxyalkanoates: Chiral Hydroxyalkanoic Acids and Polymer Secretion

Screening method to prioritize relevant bio‐based acids and their biochemical processes using recent patent information

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