Heterologous protein production in the yeast<i>Kluyveromyces lactis</i>

Ooyen, A.J.J. van; Dekker, P.; Huang, Michael; Olsthoorn, Maurien M. A.; Jacobs, Denise I.; Colussi, Paul A.; Taron, Christopher H.

doi:10.1111/j.1567-1364.2006.00049.x

Cited by 185 publications

(140 citation statements)

References 60 publications

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“…It is one of the few yeasts capable of growing on lactose as the sole carbon source and for this reason it was studied as a model for lactose metabolism and exploited as a source of eukaryotic b-galactosidase. Another field of application of this yeast is the production of heterologous proteins (van Ooyen et al, 2006). Its genome has been sequenced and annotated (Dujon et al, 2004) and is available at http://cbi.labri.fr/Genolevures.…”

Section: Introductionmentioning

confidence: 99%

A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis

et al. 2012

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A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis In the respiratory yeast Kluyveromyces lactis, little is known about the factors regulating the metabolic response to oxygen shortage. After searching for homologues of characterized Saccharomyces cerevisiae regulators of the hypoxic response, we identified a gene that we named KlMGA2, which is homologous to MGA2. The deletion of KlMGA2 strongly reduced both the fermentative and respiratory growth rate and altered fatty acid composition and the unsaturation index of membranes. The reciprocal heterologous expression of MGA2 and KlMGA2 in the corresponding deletion mutant strains suggested that Mga2 and KlMga2 are functional homologues. KlMGA2 transcription was induced by hypoxia and the glucose sensor Rag4 mediated the hypoxic induction of KlMGA2. Transcription of lipid biosynthetic genes KlOLE1, KlERG1, KlFAS1 and KlATF1 was induced by hypoxia and was dependent on KlMga2, except for KlOLE1. Rag4 was required for hypoxic induction of transcription for both KlMga2-dependent (KlERG1) and KlMga2-independent (KlOLE1) structural genes.

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Section: Introductionmentioning

confidence: 99%

A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis

et al. 2012

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“…Most production using recombinant K. lactis has been conducted in batch proces$es [3,4]. However, nutrient limitation and starvation often lead to "stuck" batch fermentation.…”

mentioning

confidence: 99%

Simplified feeding strategies for the fed-batch cultivation of Kluyveromyces lactis GG799 for enhanced recombinant xylanase production

Fuzi

Razali

Jahim

et al. 2014

Bioprocess Biosyst Eng

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“…So promoter with TetR-binding sites has been used to control different genes in different yeasts (Blount et al, 2012b). Still, non-conventional yeast promoters are poorly characterised, and findings from S. cerevisiae can be applicable to the most the highly efficient non-conventional yeast systems also because most of the yeast promoters are capable of cross recognition (Van Ooyen et al, 2006).…”

Section: Available Promoters In Non-conventional Yeast and Promoter Ementioning

confidence: 99%

Synthetic Biology and Metabolic Engineering Approaches and Its Impact on Non-Conventional Yeast and Biofuel Production

et al. 2017

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The increasing fossil fuel scarcity has led to an urgent need to develop alternative fuels. Currently microorganisms have been extensively used for the production of first-generation biofuels from lignocellulosic biomass. Yeast is the efficient producer of bioethanol among all existing biofuels option. Tools of synthetic biology have revolutionized the field of microbial cell factories especially in the case of ethanol and fatty acid production. Most of the synthetic biology tools have been developed for the industrial workhorse Saccharomyces cerevisiae. The non-conventional yeast systems have several beneficial traits like ethanol tolerance, thermotolerance, inhibitor tolerance, genetic diversity, etc., and synthetic biology have the power to expand these traits. Currently, synthetic biology is slowly widening to the non-conventional yeasts like Hansenula polymorpha, Kluyveromyces lactis, Pichia pastoris, and Yarrowia lipolytica. Herein, we review the basic synthetic biology tools that can apply to non-conventional yeasts. Furthermore, we discuss the recent advances employed to develop efficient biofuel-producing non-conventional yeast strains by metabolic engineering and synthetic biology with recent examples. Looking forward, future synthetic engineering tools' development and application should focus on unexplored non-conventional yeast species.

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Heterologous protein production in the yeastKluyveromyces lactis

Cited by 185 publications

References 60 publications

A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis

A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis

Simplified feeding strategies for the fed-batch cultivation of Kluyveromyces lactis GG799 for enhanced recombinant xylanase production

Synthetic Biology and Metabolic Engineering Approaches and Its Impact on Non-Conventional Yeast and Biofuel Production

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