Heterology expression of the tomato LeLhcb2 gene confers elevated tolerance to chilling stress in transgenic tobacco

Deng, Yong-Sheng; Kong, Fanying; Zhou, Bin; Zhang, Song; Yue, Mengmeng; Meng, Qingwei

doi:10.1016/j.plaphy.2014.04.017

Cited by 33 publications

(13 citation statements)

References 31 publications

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“…Deng et al transferred the LeLhcb2 gene into tobacco and overexpressed it, and found that the overexpression of LeLhcb2 played a key role in reducing PSII photooxidation and enhancing the tolerance of transgenic tobacco to low temperature stress. These findings further confirm the role of this gene family in adaptation to environmental stress [ 35 ].…”

Section: Discussionsupporting

confidence: 81%

Circadian rhythm response and its effect on photosynthetic characteristics of the Lhcb family genes in tea plant

Hu,

Zhang,

Qin

et al. 2024

BMC Plant Biol

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Background The circadian clock, also known as the circadian rhythm, is responsible for predicting daily and seasonal changes in the environment, and adjusting various physiological and developmental processes to the appropriate times during plant growth and development. The circadian clock controls the expression of the Lhcb gene, which encodes the chlorophyll a/b binding protein. However, the roles of the Lhcb gene in tea plant remain unclear. Results In this study, a total of 16 CsLhcb genes were identified based on the tea plant genome, which were distributed on 8 chromosomes of the tea plant. The promoter regions of CsLhcb genes have a variety of cis-acting elements including hormonal, abiotic stress responses and light response elements. The CsLhcb family genes are involved in the light response process in tea plant. The photosynthetic parameter of tea leaves showed rhythmic changes during the two photoperiod periods (48 h). Stomata are basically open during the day and closed at night. Real-time quantitative PCR results showed that most of the CsLhcb family genes were highly expressed during the day, but were less expressed at night. Conclusions Results indicated that CsLhcb genes were involved in the circadian clock process of tea plant, it also provided potential references for further understanding of the function of CsLhcb gene family in tea plant.

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Section: Discussionsupporting

confidence: 81%

Circadian rhythm response and its effect on photosynthetic characteristics of the Lhcb family genes in tea plant

Hu,

Zhang,

Qin

et al. 2024

BMC Plant Biol

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“…Structural and functional analyses of LHC superfamily members showed their involvement in light-harvesting and response to various stresses. The overexpression of tomato LeLhcb2 in tobacco revealed an elevated tolerance to chilling stress and the alleviated photo-oxidation of PSII [ 11 ]. Enhanced expressions of tomato LHC genes in RNAi- SlBEL11 tomato fruits were related to increased chlorophyll contents [ 12 ], while overexpression of Sedum alfredii SaLhcb2 in tobacco increased the shoot biomass and higher Cd 2+ accumulation [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content

Luo

Abid

et al. 2022

IJMS

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Light-harvesting chlorophyll a/b-binding (LHC) protein is a superfamily that plays a vital role in photosynthesis. However, the reported knowledge of LHCs in kiwifruit is inadequate and poorly understood. In this study, we identified 42 and 45 LHC genes in Actinidia chinensis (Ac) and A. eriantha (Ae) genomes. Phylogenetic analysis showed that the kiwifruit LHCs of both species were grouped into four subfamilies (Lhc, Lil, PsbS, and FCII). Expression profiles and qRT-PCR results revealed expression levels of LHC genes closely related to the light, temperature fluctuations, color changes during fruit ripening, and kiwifruit responses to Pseudomonas syringae pv. actinidiae (Psa). Subcellular localization analysis showed that AcLhcb1.5/3.1/3.2 were localized in the chloroplast while transient overexpression of AcLhcb3.1/3.2 in tobacco leaves confirmed a significantly increased content of chlorophyll a. Our findings provide evidence of the characters and evolution patterns of kiwifruit LHCs genes in kiwifruit and verify the AcLhcb3.1/3.2 genes controlling the chlorophyll a content.

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“…The LHC is widely involved in the capture and transfer of light energy, leaf development, maintenance of vesicle membrane structure, and various stress responses [54,55]. For instance, overexpression of the LHCB2 gene in tobacco enhanced tolerance to low temperatures [29]. Fe de ciency induces phosphorylation of HvLHCB1 proteins, leading to their migration from grana stacks to stroma thylakoid membranes, thereby optimizing the excitation balance between PSII and PSI[56].…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, numerous LHCII genes have been found to regulate responses to abiotic stress [26,28]. Overexpression of LeLHCB2 in tomato lessens the accumulation of ROS under low-temperature stress and enhances the low-temperature tolerance of transgenic tobacco [29]. The PSII antenna proteins CP29 and CP47 of Salicornia europaea, as well as several LHCII proteins, showed increased abundance in response to salt treatment, which enhanced the excitation energy capture capacity and e ciency of PSII [30].…”

Section: Introductionmentioning

confidence: 99%

The MhLHCB15 gene interacts with interacting proteins to regulate iron deficiency tolerance in Malus halliana

Dong,

Zhang,

Cheng

et al. 2024

Preprint

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Background Iron (Fe) plays a crucial role as a micronutrient in facilitating plant growth and development. Alterations in the availability of iron can trigger a response resulting in iron deficiency, ultimately affecting both plant growth and crop yield. Results A total of 33 MhLHC family members were identified in Malus halliana based on the apple genome database under Fe deficiency. The study comprehensively analyzed the physicochemical properties, gene structures, conserved motif compositions, evolutionary relationships, and chromosomal distributions of these family members. Additionally, 15 genes were selected for qRT-PCR to verify whether they responded to abiotic stress. It was demonstrated that MhLHCB15 significantly responded to Fe deficiency stress. Among them, Further investigation was conducted on the functional properties of MhLHCB15 in Arabidopsis and apple calli. Overexpression of MhLHCB15 enhanced Fe deficiency tolerance of in both Arabidopsis and apple calli, which evoked a variety of biochemical changes: transgenic Arabidopsis displayed higher photosynthetic efficiency (Tr, Gs, and Pn) and fluorescence parameters (qP, F0, Fm, and Fv/Fm) and lower Ci compared to the WT. The chlorophyll content, photosynthetic products (SS, St and SP), and enzyme activities (SOD, POD and CAT) of transgenic A. thaliana and apple calli were markedly higher compared to the WT, however, the MDA content and ROS accumulation were less than the WT. Moreover, the FCR activity of transgenic Arabidopsis and apple calli was stronger in comparison to the WT. Conclusions In summary, overexpression of the MhLHCB15 gene enhanced Arabidopsis and apple calli tolerance to Fe deficiency stress. In this study, the interaction proteins MhSGR1 and MhTHF1 of MhLHCB15 were screened, which contributes to further analyze the regulation of MhLHCB15 on Fe deficiency tolerance of M. halliana.

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Heterology expression of the tomato LeLhcb2 gene confers elevated tolerance to chilling stress in transgenic tobacco

Cited by 33 publications

References 31 publications

Circadian rhythm response and its effect on photosynthetic characteristics of the Lhcb family genes in tea plant

Circadian rhythm response and its effect on photosynthetic characteristics of the Lhcb family genes in tea plant

Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content

The MhLHCB15 gene interacts with interacting proteins to regulate iron deficiency tolerance in Malus halliana

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