Heterophil nature of EVI antibody in Trypanosoma cruzi infection

Khoury, Emilio L.; Diez, C.; Cossio, Patricio M.; Arana, Roberto M.

doi:10.1016/0090-1229(83)90078-8

Cited by 47 publications

(19 citation statements)

References 9 publications

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“…The bulk of evidence supports the participation of T cells rather than antibody in heart damage (2,3,5,10,35). The association of cardiac myosin HC-B13 crossreactive antibodies with CCC may be only signaling the presence of "helper" T cells of similar specificity, these ones potentially involved in heart tissue damage.…”

Section: Resultsmentioning

confidence: 99%

“…Peptides pl-20 and p1439-1453 are derived from the sequence of human cardiac myosin HC, (3 isoform, and contain B13-homologous sequences (bold-faced type). The nonrelated peptide (NR) represents residues [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41] of the human T-cell receptor variable-region a-subunit 13 chain.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Autoimmunity in Chagas disease cardiopathy: biological relevance of a cardiac myosin-specific epitope crossreactive to an immunodominant Trypanosoma cruzi antigen.

Cunha-Neto¹,

Duranti²,

Gruber³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

190

153

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Heart tissue destruction in chronic Chagas disease cardiopathy (CCC) may be caused by autoimmune recognition of heart tissue by a mononuclear cell infiltrate decades after Trypanosoma cruzi infection. Indirect evidence suggests that there is antigenic crossreactivity between T. cruzi and heart tissue. As there is evidence for immune recognition of cardiac myosin in CCC, we searched for a putative myosin-crossreactive T. cruzi antigen. T. cruzi lysate immunoblots were probed with anti-cardiac myosin heavy chain IgG antibodies (AMA) affinity-purified from CCC or asymptomatic Chagas disease patient-seropositive sera. A 140/116-kDa doublet was predominantly recognized by AMA from CCC sera. Further, recombinant T. cruzi protein B13--whose native protein is also a 140- and 116-kDa double band--was identified by crossreactive AMA. Among 28 sera tested in a dot-blot assay, AMA from 100% of CCC sera but only 14% of the asymptomatic Chagas disease sera recognized B13 protein (P = 2.3 x 10(-6)). Sequence homology to B13 protein was found at positions 8-13 and 1442-1447 of human cardiac myosin heavy chain. Competitive ELISA assays that used the correspondent myosin synthetic peptides to inhibit serum antibody binding to B13 protein identified the heart-specific AAALDK (1442-1447) sequence of human cardiac myosin heavy chain and the homologous AAAGDK B13 sequence as the respective crossreactive epitopes. The recognition of a heart-specific T. cruzi crossreactive epitope, in strong association with the presence of chronic heart lesions, suggests the involvement of crossreactivity between cardiac myosin and B13 in the pathogenesis of CCC.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Autoimmunity in Chagas disease cardiopathy: biological relevance of a cardiac myosin-specific epitope crossreactive to an immunodominant Trypanosoma cruzi antigen.

Cunha-Neto¹,

Duranti²,

Gruber³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

190

153

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“…The first autoantibody to be described was one that reacted to endocardium, blood vessels and interstitium of skeletal muscle (EVI) (Cossio, et al, 1974), but was the same group of investigators who recognized the heterophil nature of the antibody and realized that had no pathogenic role (Khoury, et al, 1983). Another autoantibody, studied by our group, was anti-laminin antibody (Sanchez, Milei et al 1993, .…”

Section: Humoral Adaptative Immunitymentioning

confidence: 92%

Pathogenesis and Pathology of Chagas' Chronic Myocarditis

González¹,

Guerri-Guttenberg²,

Grana³

et al. 2011

Myocarditis

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“…As a probable autoimmune reaction is present in CHDis [14], a reliable and simple technique like this would be welcome. Fur-thermorc, files may be reviewed, and formal dehyde-fixed material, both human and ex perimental [18,19], used for different pur poses in the study of ChrCHM and other heart diseases (see above), could be studied by pathologists with advantage over immu nofluorescence.…”

Section: Discussionmentioning

confidence: 99%

Chronic Chagas’ Myocardiopathy

Molina¹,

Milei²,

Storino³

1984

Cardiology

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Chagas’ disease (CHDis) has been described as the commonest form of chronic myocarditis. Although autoimmune phenomena involving the myocardium have been suggested in both human and experimental CHDis, no immunoperoxidase techniques have been used for the detection of immunological deposits in chronic Chagas’ myocardiopathy (ChrCHM). The goal of this paper was to show that there is a coincidence between the immunoperoxidase and the immunofluorescence techniques in evidencing autologous deposits in ChrCHM. 4 patients suffering from ChrCHM were selected; their ages ranged from 39 to 52 years (x: 48.5 years). The Shültze and Caves endocavitary bioptome via the right internal jugular vein was used to obtain 3 to 4 right endomyocardial specimens measuring 1–2 mm each. Direct immunofluorescence examination, immunoperoxidase technique (PAP), and routine histological procedure were performed in all cases. Agreement between immunofluorescence and immunoperoxidase techniques was present in all chagasic specimens studied. Patients suffering from ChrCHM showed deposits of IgG, IgA, IgM, and C3 at the interstitial area, capillaries and endocardium and/or myocardial fiber surfaces when serum EVI antibody was positive. Serum EVI antibody was found to be positive in 3 out of 4 patients. The presence of the complement together with immunoglobulin may suggest an immune reaction (in vivo bound immunoglobulins in heart structures) and not just a passive deposition secondary to myocardial damage. As EVI antibody is present to a significant extent in CHDis, a reliable and simple technique like immunoperoxidase may be helpful to clarify the role of these antibodies in the pathogenesis of CHDis.

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Heterophil nature of EVI antibody in Trypanosoma cruzi infection

Cited by 47 publications

References 9 publications

Autoimmunity in Chagas disease cardiopathy: biological relevance of a cardiac myosin-specific epitope crossreactive to an immunodominant Trypanosoma cruzi antigen.

Autoimmunity in Chagas disease cardiopathy: biological relevance of a cardiac myosin-specific epitope crossreactive to an immunodominant Trypanosoma cruzi antigen.

Pathogenesis and Pathology of Chagas' Chronic Myocarditis

Chronic Chagas’ Myocardiopathy

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Heterophil nature of EVI antibody in Trypanosoma cruzi infection

Cited by 47 publications

References 9 publications

Autoimmunity in Chagas disease cardiopathy: biological relevance of a cardiac myosin-specific epitope crossreactive to an immunodominant Trypanosoma cruzi antigen.

Autoimmunity in Chagas disease cardiopathy: biological relevance of a cardiac myosin-specific epitope crossreactive to an immunodominant Trypanosoma cruzi antigen.

Pathogenesis and Pathology of Chagas' Chronic Myocarditis

Chronic Chagas&rsquo; Myocardiopathy

Contact Info

Product

Resources

About

Chronic Chagas’ Myocardiopathy