Heteroresistance to carbapenems in invasive Pseudomonas aeruginosa infections

He, Jiaxun; Jia, Xiaojiong; Yang, Shuangshuang; Xu, Xun; Sun, Kunling; Li, Congya; Yang, Tianxiang; Zhang, Liping

doi:10.1016/j.ijantimicag.2017.10.014

Cited by 44 publications

(44 citation statements)

References 28 publications

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“…The molecular epidemiology of all the CRKA strains was determined by pulsed-field gel electrophoresis (PFGE) after total chromosomal DNA digestion with XbaI in accordance with a previous report. 11 The genomic DNA restriction patterns of the isolates were analyzed and interpreted according to the previously proposed criteria. 12…”

Section: Pfgementioning

confidence: 99%

Carbapenem-Resistant Klebsiella aerogenes Clinical Isolates from a Teaching Hospital in Southwestern China: Detailed Molecular Epidemiology, Resistance Determinants, Risk Factors and Clinical Outcomes

Ma¹,

Huang²,

Zhang³

et al. 2020

IDR

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Little is known about the epidemiology and carbapenem-resistance determinants of carbapenem-resistant K. aerogenes (CRKA) isolated from a single medical center. The present study was initiated to characterize the molecular epidemiology and the carbapenemresistance mechanisms of CRKA isolated during 2012-2018 from a teaching hospital in southwest China, and to investigate the risk factors and clinical outcomes of CRKA infections as well. Methods: Pulsed-field gel electrophoresis (PFGE) was employed for epidemiological analysis. PCR amplification and DNA sequencing were used to examine the antibiotic-resistance determinants. Plasmids were extracted and characterized by PCR-based replicon typing and conjugation assays. In order to further investigate the risk factors and clinical outcomes of CRKA infections, a retrospective case-control study was also performed. Results: PFGE analysis showed 32 different PFGE patterns among the 36 non-duplicated CRKA strains collected. Most of the isolates harbored multi-drug resistance (MDR) genes, including 2 (5.6%) carrying bla NDM-1 , 1 (2.8%) harboring bla KPC-2 , 13 (36.1%) carrying ESBL genes, 23 (63.9%) carrying ampC genes, 34 (94.4%) carrying quinolone resistance determinants (QRD) genes and 9 (25%) carrying aminoglycoside resistance determinants (ARD) genes. The outer membrane porins, OmpE35 and OmpE36, were, respectively, lost in 4 and 2 isolates. The efflux pump inhibition experiments were positive in 25 (69.4%) of the CRKA strains. Multivariate analysis indicated that hypo-albuminaemia, invasive procedures, and carbapenem exposure were independent risk factors for acquiring CRKA infections. Conclusion: No clonality relationship was identified among most of the 36 CRKA isolates. The over-expression of ESBLs and AmpC coupled with the efflux pumps contributed to carbapenem resistance in K. aerogenes. Additionally, this is the first report of CRKA isolate co-harboring bla NDM-1 , bla CTX-M-15 , bla EBC , bla ACC , acc (6ʹ)-Ib, armA, qnrD and loss of OmpE36 in China. Hypo-albuminaemia, invasive procedures and carbapenem exposure were associated with acquisition of CRKA infections.

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Section: Pfgementioning

confidence: 99%

Carbapenem-Resistant Klebsiella aerogenes Clinical Isolates from a Teaching Hospital in Southwestern China: Detailed Molecular Epidemiology, Resistance Determinants, Risk Factors and Clinical Outcomes

Ma¹,

Huang²,

Zhang³

et al. 2020

IDR

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“…However, it poses a major threat to immunocompromised individuals and cystic fibrosis patients with underlying conditions [ 4 ]. Furthermore, P. aeruginosa can thrive and reproduce easily, showing strong resistance to antibiotics and a tendency to generate acquired resistance [ 5 ]. The threat can lead to high mortality and morbidity [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, as a pathogen of the family Pseudomonadaceae , P. aeruginosa is able to thrive and reproduce easily in a wide range of environmental conditions, showing strong resistance to chemical and physical factors such as disinfectants and ultraviolet radiation [ 7 , 8 ]. Therefore, the various adaptive responses and genetic mutations result in P. aeruginosa having strong resistance to antibiotics and a tendency to generate acquired resistance [ 5 , 9 ]. To treat P. aeruginosa contamination, novel antimicrobial approaches are necessary.…”

Section: Introductionmentioning

confidence: 99%

A Multi-Scale Approach to Investigate Adhesion Properties of Pseudomonas aeruginosa PAO1 to Geotrichum candidum LG-8, a Potential Probiotic Yeast

Zhou

et al. 2020

Foods

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This study investigated properties of Pseudomonas aeruginosa PAO1 adhesion to Geotrichum candidum LG-8 cells in variable pH and salt conditions. The primary mechanism was revealed by multi-scale microscopy technics. The adhesion of PAO1 to the living fungus occurred within 1 h and was limited at concentrations of bile salts higher than 0.5%. The adhesion efficiency gradually increased to 58.1% with the pH increasing from 2.0 to 7.0 and then decreased to 48.2% at pH 9.0. However, the dead LG-8 has an advantage over the living ones to adhere PAO1 in same pH and bile salt conditions. Optical microscopy showed that both unsterilized and sterilized G. candidum LG-8 cells removed approximately one hundred fold bacteria in 4 h. Laser scanning confocal microscopy (LSCM) analysis indicated that polysaccharides of the fungus contributed to adhesion. Scanning electron microscopy (SEM) analysis proved that syrup-like EPS (extracellular polymeric substances) of LG-8 coating PAO1 was in part a mechanism. Atomic force microscopy (AFM) showed roughness of the LG-8 surface changed in the adhesion process. Furthermore, a pedestal-like structure of bacteria was observed by transmission electron microscopy (TEM) analysis, indicating that the bacteria were also actively involved in the adhesion process. G. candidum LG-8 is a potential candidate for the control of P. aeruginosa PAO1 in the food industry and immunodeficiency patients.

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“…Pseudomonas aeruginosa is a ubiquitous, clinically significant, Gram-negative bacillus and an opportunistic human pathogen (Stover et al, 2000; Lee et al, 2018). P. aeruginosa infections are particularly difficult to eradicate because this pathogen is prone to acquire resistance to multiple antibiotics and it prefers to form antibiotic-resistant biofilms (Galloway et al, 2012; He et al, 2018; Li et al, 2018). In recent years, a number of studies have reported that garlic extract contains P. aeruginosa QS inhibitors, including ajoene, a sulfur compound from garlic (Rasmussen et al, 2005; Bodini et al, 2009; Harjai et al, 2010; Jakobsen et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Diallyl Disulfide From Garlic Oil Inhibits Pseudomonas aeruginosa Quorum Sensing Systems and Corresponding Virulence Factors

Xie

et al. 2019

Front. Microbiol.

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Previously, we determined that diallyl disulfide (DADS) from garlic oil can inhibit Pseudomonas aeruginosa PAO1 pathogenic factors by inactivating the transcription of key genes from three quorum sensing (QS) systems (las, rhl, and pqs) based on the effects of DADS on growth, virulence factor production (elastase, pyocyanin, biofilm, and swarming motility), and RNA transcription (real-time q-PCR). To further investigate the mechanisms underlying the inhibition of the three P. aeruginosa QS systems by DADS, high-throughput RNA and proteome sequencing techniques were used to study differences in the transcriptional and proteome expression of P. aeruginosa PAO1 following treatment with DADS. The RNA-seq and proteomic data are available via NCBI Gene Expression Omnibus database with accession number GSE118801 and ProteomeXchange with identifier PXD011144, respectively. The experimental results indicated that all key genes of the three QS systems (las, rhl, and pqs) of P. aeruginosa PAO1 as well as the virulence factors (including exoprotease LasA, elastase LasB, lectin LecA and LecB, pyocyanin biosynthesis, and biofilm formation) regulated by these three QS systems were inhibited. This is consistent with our previous studies on the physiology, biochemistry, and RNA expression of P. aeruginosa treated with DADS. Additionally, our results also indicated that bacterial motility, chemotaxis, and two-component systems were inhibited by DADS treatment. All these changes abolish the sensitivity of P. aeruginosa PAO1 to environmental stimuli and cause the cells to be in a state of passivation. Further research is needed to determine how QS systems regulate these functions. Our findings could potentially contribute to the treatment and control of P. aeruginosa infection, virulence, and pathogenicity.

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Heteroresistance to carbapenems in invasive Pseudomonas aeruginosa infections

Cited by 44 publications

References 28 publications

<p>Carbapenem-Resistant <em>Klebsiella aerogenes</em> Clinical Isolates from a Teaching Hospital in Southwestern China: Detailed Molecular Epidemiology, Resistance Determinants, Risk Factors and Clinical Outcomes</p>

<p>Carbapenem-Resistant <em>Klebsiella aerogenes</em> Clinical Isolates from a Teaching Hospital in Southwestern China: Detailed Molecular Epidemiology, Resistance Determinants, Risk Factors and Clinical Outcomes</p>

A Multi-Scale Approach to Investigate Adhesion Properties of Pseudomonas aeruginosa PAO1 to Geotrichum candidum LG-8, a Potential Probiotic Yeast

Diallyl Disulfide From Garlic Oil Inhibits Pseudomonas aeruginosa Quorum Sensing Systems and Corresponding Virulence Factors

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