Heterosis for superoxide dismutase, peroxidase and catalase enzymes in the head of single cross-hybrids of cabbage (Brassica oleracea var. capitata)

Singh, Bhupinder

doi:10.1007/s12041-010-0028-8

Cited by 7 publications

(5 citation statements)

References 12 publications

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“…Crossbreeding is a means to promote recombination of parental genes, and is also a prerequisite for breeding superior offspring, that not only inherit desired traits from both parental lines, but also can exhibit novel F 1 phenotypes, heterosis and modified gene expression ( Shivaprasad et al, 2012 ; Bell et al, 2013 ). However, the role of heterosis in defense mechanism of abiotic stress is still poorly understood ( Dong et al, 2006 ; Korn et al, 2008 ; Singh, Sharma & Singh, 2010 ; Miller et al, 2015 ). Korn et al (2008) showed that there is a strikingly strong correlation between heterosis, freezing tolerance, and flavonol content.…”

Section: Discussionmentioning

confidence: 99%

“… Korn et al (2008) showed that there is a strikingly strong correlation between heterosis, freezing tolerance, and flavonol content. The heterotic vigor for SOD, POD and CAT suggests an improvement of stress tolerance level in hybrids compared to the parental lines ( Singh, Sharma & Singh, 2010 ). Miller et al (2015) found that the levels of stress-responsive gene expression in parental lines could be used to predict biomass heterosis in hybrids.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.)

Shi

Cheng

Wen

et al. 2018

PeerJ

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Drought stress is one of the most important abiotic factors limiting crop productivity. A better understanding of the effects of drought on millet (Setaria italica L.) production, a model crop for studying drought tolerance, and the underlying molecular mechanisms responsible for drought stress responses is vital to improvement of agricultural production. In this study, we exposed the drought resistant F1 hybrid, M79, and its parental lines E1 and H1 to drought stress. Subsequent physiological analysis demonstrated that M79 showed higher photosynthetic energy conversion efficiency and drought tolerance than its parents. A transcriptomic study using leaves collected six days after drought treatment, when the soil water content was about ∼20%, identified 3066, 1895, and 2148 differentially expressed genes (DEGs) in M79, E1 and H1 compared to the respective untreated controls, respectively. Further analysis revealed 17 Gene Ontology (GO) enrichments and 14 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in M79, including photosystem II (PSII) oxygen-evolving complex, peroxidase (POD) activity, plant hormone signal transduction, and chlorophyll biosynthesis. Co-regulation analysis suggested that these DEGs in M79 contributed to the formation of a regulatory network involving multiple biological processes and pathways including photosynthesis, signal transduction, transcriptional regulation, redox regulation, hormonal signaling, and osmotic regulation. RNA-seq analysis also showed that some photosynthesis-related DEGs were highly expressed in M79 compared to its parental lines under drought stress. These results indicate that various molecular pathways, including photosynthesis, respond to drought stress in M79, and provide abundant molecular information for further analysis of the underlying mechanism responding to this stress.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.)

Shi

Cheng

Wen

et al. 2018

PeerJ

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“…Genes have been identified that could be used to enhance crop yield and quality [43]. Among them, SOD , APX , and CAT have been introduced into many crops to improve stress tolerance [6], [13], [15], [18], [20], [22]–[25], [27]–[30], [35]–[38]. In this study, we isolated GhSOD1 , GhAPX1 , and GhCAT1 from cotton and produce five types of transgenic cotton plants overexpressing theses genes individually or in combination in chloroplasts.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effects of GhSOD1 and GhCAT1 Overexpression in Cotton Chloroplasts on Enhancing Tolerance to Methyl Viologen and Salt Stresses

Luo

et al. 2013

PLoS ONE

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In plants, CuZn superoxide dismutase (CuZnSOD, EC l.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), and catalase (CAT, EC l.11.1.6) are important scavengers of reactive oxygen species (ROS) to protect the cell from damage. In the present study, we isolated three homologous genes (GhSOD1, GhAPX1, and GhCAT1) from Gossypium hirsutum. Overexpressing cassettes containing chimeric GhSOD1, GhAPX1, or GhCAT1 were introduced into cotton plants by Agrobacterium transformation, and overexpressed products of these genes were transported into the chloroplasts by transit peptide, as expected. The five types of transgenic cotton plants that overexpressed GhSOD1, GhAPX1, GhCAT1, GhSOD1 and GhAPX1 stack (SAT), and GhSOD1 and GhCAT1 stack (SCT) were developed. Analyses in the greenhouse showed that the transgenic plants had higher tolerance to methyl viologen (MV) and salinity than WT plants. Interestingly, SCT plants suffered no damage under stress conditions. Based on analyses of enzyme activities, electrolyte leakage, chlorophyll content, photochemical yield (Fv/Fm), and biomass accumulation under stresses, the SCT plants that simultaneously overexpressed GhSOD1 and GhCAT1 appeared to benefit from synergistic effects of two genes and exhibited the highest tolerance to MV and salt stress among the transgenic lines, while the SAT plants simultaneously overexpressing GhSOD1 and GhAPX1 did not. In addition, transgenic plants overexpressing antioxidant enzymes in their chloroplasts had higher tolerance to salt stress than those expressing the genes in their cytoplasms, although overall enzyme activities were almost the same. Therefore, the synergistic effects of GhSOD1 and GhCAT1 in chloroplasts provide a new strategy for enhancing stress tolerance to avoid yield loss.

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“…capitata L.), a member of Brassicaceae family, is an economically and nutritionally important vegetable crop that is grown and consumed widely around the globe (Chiang et al, 1993). Cabbage is gaining popularity worldwide because it is inexpensive, has wider adaptability, and is available year-round (Singh et al, 2010a). It is cultivated for its heads, which are consumed fresh in salads and in different culinary dishes and also preserved by pickling, dehydration or freezing (Posta and Berar, 2006).…”

Section: Introductionmentioning

confidence: 99%

Heterosis for yield contributing head traits in cabbage (Brassica oleracea var. capitata)

2015

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Hybrids and parents were evaluated in a randomized block design with three replications. Measurements were performed for head weight, head diameter and head length to estimate mid parent and better parent heterosis in each hybrid. The direction and magnitude of mid parent and better parent heterosis among hybrids for all the head traits was found to be highly variable. The maximum and significant heterosis in favorable directions both over mid parent and better parent for head weight (73.6 and 62.3%, respectively), head diameter (39.6 and 39.1%, respectively) and head length (25.3 and 21.6%, respectively) was observed in the hybrid P8 × P14. In this study, the hybrids P8 × P14, P3 × P13, P3 × P14 and P8 × P13 were found to be promising hybrid combinations with regard to their per se performance for head traits and the magnitude of heterosis. These hybrids can be selected for commercial exploitation of hybrid vigor. Thus, the information generated from this study might be vital and useful for breeders to develop hybrid varieties with better head traits and high yield through heterosis breeding.

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Heterosis for superoxide dismutase, peroxidase and catalase enzymes in the head of single cross-hybrids of cabbage (Brassica oleracea var. capitata)

Cited by 7 publications

References 12 publications

Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.)

Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.)

Synergistic Effects of GhSOD1 and GhCAT1 Overexpression in Cotton Chloroplasts on Enhancing Tolerance to Methyl Viologen and Salt Stresses

Heterosis for yield contributing head traits in cabbage (Brassica oleracea var. capitata)

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