2018
DOI: 10.1073/pnas.1805713115
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Heterosubtypic influenza protection elicited by double-layered polypeptide nanoparticles in mice

Abstract: Influenza is a persistent threat to public health. Here we report that double-layered peptide nanoparticles induced robust specific immunity and protected mice against heterosubtypic influenza A virus challenges. We fabricated the nanoparticles by desolvating a composite peptide of tandem copies of nucleoprotein epitopes into nanoparticles as cores and cross-linking another composite peptide of four tandem copies of influenza matrix protein 2 ectodomain epitopes to the core surfaces as a coating. Delivering th… Show more

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Cited by 84 publications
(95 citation statements)
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“…Coated and dissolvable MNs represent dry formulations, which compared to liquid-based injectable vaccines are easier to store, require lesser space and weight capacity for storage, and have reduced cost of transport. Such designs have been developed for viruses including influenza, 22,[26][27][28][29][30][31][32][33][34][35][36][37] hepatitis B, [38][39][40][41] hepatitis C, 42 polio, 43,44 herpes simplex, 45,46 human papillomavirus, 47,48 rotavirus, 49 measles and rubella, 50-52 rabies, 53 HIV, 54,55 Ebola, 56 chikungunya, and West Nile virus. 57 However despite extensive advances in MN-based vaccines, there are limited studies addressing the delivery of LAVs.…”
Section: Resultsmentioning
confidence: 99%
“…Coated and dissolvable MNs represent dry formulations, which compared to liquid-based injectable vaccines are easier to store, require lesser space and weight capacity for storage, and have reduced cost of transport. Such designs have been developed for viruses including influenza, 22,[26][27][28][29][30][31][32][33][34][35][36][37] hepatitis B, [38][39][40][41] hepatitis C, 42 polio, 43,44 herpes simplex, 45,46 human papillomavirus, 47,48 rotavirus, 49 measles and rubella, 50-52 rabies, 53 HIV, 54,55 Ebola, 56 chikungunya, and West Nile virus. 57 However despite extensive advances in MN-based vaccines, there are limited studies addressing the delivery of LAVs.…”
Section: Resultsmentioning
confidence: 99%
“…As alternatives to CMC, chitosan in combination with PLA and sucrose in combination with threonine as well as PVA have been used to make fully dissolvable microneedles. Recently, Wang and Prausnitz developed a dissolvable microneedle made from PVA and sucrose to deliver peptide nanoparticles as a vaccine against H1N1 influenza . One notable challenge with most polysaccharide microneedles is their weak mechanical properties (e.g., softness and sometimes brittleness).…”
Section: Macroscale Materials For Immunotherapymentioning
confidence: 99%
“…Some conserved HLA I and II-recognized epitopes have been characterized. Fusion peptides and recombinant proteins including these epitopes have been studied in laboratory animals and showed strong T cell responses and broad protection [ 42 , 43 , 44 , 45 ]. However, novel approaches are to be investigated to identify and utilize new NP T cell epitopes to design novel immunogens for a universal influenza vaccine.…”
Section: Antigenic Structures Conserved Over Different Influenza Tmentioning
confidence: 99%