Heterotopic ovarian allotransplantation in goats: Preantral follicle viability and tissue remodeling

Pinto, Yago; Alves, Kele Amaral; Alves, Bênner Geraldo; Souza, Samara Silva de; Brandão, Fabiana Aparecida Santilli; Lima, Laritza Ferreira de; Freitas, V. J. F.; Rodrigues, Ana Paula Ribeiro; Figueiredo, J.R.; Gastal, E.L.; Teixeira, Dárcio Ítalo Alves

doi:10.1016/j.anireprosci.2020.106310

Cited by 9 publications

(12 citation statements)

References 62 publications

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“…The surgical procedures of transplant and harvesting were relatively simple and quickly performed but did require a well-trained operator. The subcutaneous site has been used previously for grafting of ovarian tissue in several species (mice [ 52 , 53 ], goats [ 44 ], non-human primates [ 23 ], and humans [ 54 , 55 ]). A temporary inflammatory reaction is usually expected to occur at the transplantation site during the first days post-grafting.…”

Section: Discussionmentioning

confidence: 99%

“…Herein, the use of color-Doppler ultrasonography was used as a novel non-invasive technique for monitoring the local vascular perfusion on superficial grafting sites in horses. A similar approach has been used recently by our team to monitor local blood flow on superficial ovarian grafts in goats [ 44 ]. Although lower blood flow at the transplant site was observed for 4 days after cooled ovarian fragments were grafted, local blood flow decreased to basal levels 6 days after grafting, becoming similar to that of fragments kept at room temperature.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, the lower follicular density observed in cooled fragments post-grafting was potentially due to the ischemia that occurs immediately after transplantation. Studies have demonstrated that ischemia-reperfusion and hypoxia play a major role in follicle depletion during the first days (approximately 1 week) after transplantation [ 44 , 47 , 86 ]. Altogether, the results of the present study showed that the efficiency of the cooling procedure in preserving follicular and stromal cell quality was comparable to that of fragments maintained for a short period at room temperature before transplantation.…”

Section: Discussionmentioning

confidence: 99%

“…The grafted fragments were located about 2 cm from the scanned areas R1, R2, R3, and R4. During each scanning, three different images with maximum color-Doppler signals of blood flow were obtained from each region and processed for the extraction and quantification of the number of colored pixels as described previously [ 44 ]. Then, the ImageJ ® software (National Institutes of Health, Millersville, USA), previously calibrated (1 cm = 33 pixels), was used for quantification of the area (cm 2 ) of color pixels from each image.…”

Section: Methodsmentioning

confidence: 99%

“…The total collagen fiber content in the connective tissue and the different types of collagen fibers based on polarizing colors (i.e., collagen type I fibers stained yellow with orange birefringence and collagen type III fibers stained with green birefringence) were analyzed as previously described [ 51 ]. Images were analyzed by red, green, and blue threshold measurement to obtain the percentage of red and green colors (expressed in pixels) and 3D data representation of the pixels for each image using ImageJ software [ 44 ]. The blue color representing all the other cellular types was omitted.…”

Section: Methodsmentioning

confidence: 99%

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Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF

Souza

Alves

et al. 2020

PLoS ONE

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Heterotopic and orthotopic ovarian tissue autotransplantation techniques, currently used in humans, will become promising alternative methods for fertility preservation in domestic and wild animals. Thus, this study describes for the first time the efficiency of a heterotopic ovarian tissue autotransplantation technique in a large livestock species (i.e., horses) after ovarian fragments were exposed or not to a cooling process (4°C/24 h) and/or VEGF before grafting. Ovarian fragments were collected in vivo via an ultrasound-guided biopsy pick-up method and surgically autografted in a subcutaneous site in both sides of the neck in each mare. The blood flow perfusion at the transplantation site was monitored at days 2, 4, 6, and 7 post-grafting using color-Doppler ultrasonography. Ovarian grafts were recovered 7 days post-transplantation and subjected to histological analyses. The exposure of the ovarian fragments to VEGF before grafting was not beneficial to the quality of the tissue; however, the cooling process of the fragments reduced the acute hyperemia post-grafting. Cooled grafts compared with non-cooled grafts contained similar values for normal and developing preantral follicles, vessel density, and stromal cell apoptosis; lower collagen type III fibers and follicular density; and higher stromal cell density, AgNOR, and collagen type I fibers. In conclusion, VEGF exposure before autotransplantation did not improve the quality of grafted tissues. However, cooling ovarian tissue for at least 24 h before grafting can be beneficial because satisfactory rates of follicle survival and development, stromal cell survival and proliferation, as well as vessel density, were obtained.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF

Souza

Alves

et al. 2020

PLoS ONE

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Effects of different subcutaneous sites on heterotopic autotransplantation of canine ovarian tissue

et al. 2023

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Ovarian tissue transplantation makes it possible to restore fertility; however, the success of this technique depends on the transplant region used. Therefore, this study aimed to evaluate the effect of two subcutaneous regions on canine ovarian transplantation, pinna (Pi) and neck (Ne), for 7 and 15 days. Ovaries collected by ovariosalpingohysterectomy were fragmented using a punch device. Fresh fragments were xed, and the others were immediately grafted onto the animal itself in the Pi and Ne regions for 7 and 15 days. Recovered fragments were evaluated for histology (morphology, development and stromal density), picrosirius (collagen bers), and immunohistochemistry ( brosis and cell proliferation). The results showed that follicular normality rates were lower in Pi-7 (78%) vs. control (90%) and Pi-15 (86%), similar in Ne-7 (92%) and superior in Ne-15 (97%) compared to the control, with the effect of the region Ne (94%) superior (P < 0.05) to Pi (82%). Stromal density reduced in both regions vs. control but was similar within 15 days. Fragments from both regions showed higher bronectin labeling and deposition of type I and lower type III collagen bers (P < 0.05) vs. control. Proliferation rates in Ne-7 were higher (P < 0.05) than in control, and Pi-15 was higher (P < 0.05) than Ne-15. In conclusion, the pinna can be a potential region for canine ovarian tissue autotransplantation than the neck region in 15 days.

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Heterotopic ovarian allotransplantation in a caprine model: Effects of implant site on morphological parameters

Vieira,

Pereira Bersano,

Brandão

et al. 2024

Animal Reproduction Science

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Heterotopic ovarian allotransplantation in goats: Preantral follicle viability and tissue remodeling

Cited by 9 publications

References 62 publications

Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF

Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF

Effects of different subcutaneous sites on heterotopic autotransplantation of canine ovarian tissue

Heterotopic ovarian allotransplantation in a caprine model: Effects of implant site on morphological parameters

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