Heterotopic sternum transplant in rats: A new model of a vascularized bone marrow transplantation

Santiago, S.; Faria, Werviston De; Khan, Tasnim; Gandia, Carlos; Misiakos, Evangelos P.; Ferrer, Luís Miguel; Ruiz, Phil; Coleman, Laurence; Fernandez, Hugo F.; Miller, Joshua; Ricordi, Camillo; Tzakis, Andreas G.

doi:10.1002/(sici)1098-2752(1999)19:7<330::aid-micr8>3.3.co;2-9

Cited by 11 publications

(18 citation statements)

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“…[3][4][5][6][17][18][19][20][21][22] Tai et al developed a technically simple model of vascularized femur transplantation called extraperitoneal isolated vascularized bone marrow transplant (iVBMT). 23 In iVBMT, this transplant was placed subcutaneously and resulted in minimal morbidity in the recipient animals.…”

Section: Discussionmentioning

confidence: 99%

“…23 However, these models did not include transplantation of the skin, which is an essential component in many CTA models. [3][4][5][6][17][18][19][20][21][22][23] Since we preserved the structural integrity of the mandible, we can now expect bone marrow-derived cell engraftment. In addition, the large skin component in this model will enable the monitoring of flap viability in an isotransplant setting.…”

Section: Discussionmentioning

confidence: 99%

“…These studies confirmed the superiority of VBMT for the hematopoietic reconstitution of donor bone marrow cells when compared with cellular bone marrow transplantations (BMT). [3][4][5][6][15][16][17][18][19][20][21][22][23] In their recent comprehensive review on VBMT, Gordon et al examined the newly found benefits, the unique characteristics and the applicability of VBMT as an alternative to BMT. They emphasized the importance of VBMT to potentially reduce the risk of engraftment failure, provide a more rapid engraftment, enhance cellular function, reduce graft-versus-host disease, and aid in inducing immunologic tolerance.…”

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confidence: 99%

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A New Composite Hemiface/Mandible/Tongue Transplantation Model in Rats

Külahçi

Siemionow²

2010

Annals of Plastic Surgery

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Extensive head and neck deformities, including bone and soft tissue defects, are always challenging for reconstructive surgeons. The purpose of this study was to extend the application of the face/scalp transplantation model in rats by the incorporation of vascularized mandible, masseter and tongue (based on the same vascular pedicle), and to use this as a model to test new reconstructive options for extensive head and neck deformities with involving large soft and bone tissue defects.A total of 10 composite hemiface/mandible/tongue transplantations were performed in Lewis rats (RT1). Hemimandibular bone, masseter muscle, tongue and hemifacial skin flaps were dissected based on the same vascular pedicle of common carotid artery and external jugular vein. The flaps were then transplanted to the recipient inguinal region. Evaluation methods included flap angiography, plain x-ray, computed tomographic scan, and histology.All transplants survived indefinitely and no graft loss was noted. Flap angiography demonstrated intact vascular supply to the bone. Computed tomography scan and bone histology confirmed the viability of the bone components for the composite grafts. Hematoxylin and eosin staining determined the presence of viable bone marrow cells within the transplanted mandible. Viability of the tongue was confirmed by the presence of pink color and bleeding after puncture, as well as by histology.We have introduced a new composite hemiface/mandible/tongue transplant model. The main advantage of this model is the presence of vascularized bone marrow within the mandibular component, which may facilitate future studies on chimerism and tolerance induction. Although this mandible composite allograft is placed heterotopically to the recipient inguinal region, we believe that it may serve as a new reconstructive option for the coverage of combined bone and soft tissue defects within the head and neck region.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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A New Composite Hemiface/Mandible/Tongue Transplantation Model in Rats

Külahçi

Siemionow²

2010

Annals of Plastic Surgery

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“…9 In different models, the amount of the transplanted bone marrow cells is limited since only a single bone is transplanted. [13][14][15][16] Recently, we have introduced BVFB transplant, based on the abdominal aorta and inferior vena cava, as a new model of VBMT. 20 The feasibility of BVFB transplantation was confirmed and we have concluded that it is a reliable model to study the role of bone marrow augmentation via vascularized transplantation in the induction of tolerance across MHC barriers.…”

Section: Discussionmentioning

confidence: 99%

“…9 Various models of VBMT have been used in rats: limb, sternum, and femoral bone marrow transplant. [13][14][15][16] We have previously reported successful limb allograft survival under antilymphocyte serum (ALS) and CsA protocol. The limb allograft represents a vascular bone marrow transplant as a constant source of delivery of donor-derived bone marrow cells.…”

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Applications of Bilateral Vascularized Femoral Bone Marrow Transplantation for Chimerism Induction Across the Major Histocompatibility (MHC) Barrier

Klimczak¹,

Ağaoğlu²,

Carnevale³

et al. 2006

Annals of Plastic Surgery

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Bilateral vascularized bone marrow transplant (VBMT) model was designed to induce chimerism across the major histocompatibility (MHC) barrier under combined alphabeta T-cell receptor monoclonal antibody and cyclosporine A (alphabeta-TCRmAb/CsA) protocol. Seventeen transplants were performed between BN(RT1) donors and Lewis(RTI) recipients. Group I, isograft controls; Group II, allografts rejection controls; Group III, allografts under 7-day protocol of alphabeta-TCRmAb/CsA. Donor bilateral femoral bones were bilaterally anastomosed to the abdominal aorta and inferior vena cava of recipient. At day 7 posttransplantation, all bone flaps were viable. Groups I and III survived without signs of rejection. In Group III, peak level of chimerism in peripheral blood was evaluated at day 21 (24.2%), at day 63 declined to 1.5%, and was maintained at this level thereafter. Donor-derived cells were present in the bone marrow of recipients at 28.2% at day 21 posttransplant. Histology confirmed viability of bone marrow cells in isograft during the entire follow-up and up to 35 days in treatment Group III. Bilateral VBMT induced donor-specific chimerism across the MHC barrier under the immunomodulatory protocol of alphabeta-TCRmAb/CsA.

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Allotransplantation

Siemionow¹,

Zor²

2015

Plastic and Reconstructive Surgery

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Heterotopic sternum transplant in rats: A new model of a vascularized bone marrow transplantation

Cited by 11 publications

References 0 publications

A New Composite Hemiface/Mandible/Tongue Transplantation Model in Rats

A New Composite Hemiface/Mandible/Tongue Transplantation Model in Rats

Applications of Bilateral Vascularized Femoral Bone Marrow Transplantation for Chimerism Induction Across the Major Histocompatibility (MHC) Barrier

Allotransplantation

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