Heterotrimeric G protein alpha subunit controls growth, stress response, extracellular protease activity, and cyclopiazonic acid production in Penicillium camemberti

García-Rico, Ramón O.; Gil-Durán, Carlos; Rojas-Aedo, Juan F.; Vaca, Inmaculada; Figueroa, Luis; Levicán, Gloria; Chávez, Renato

doi:10.1016/j.funbio.2017.05.007

Cited by 17 publications

(13 citation statements)

References 60 publications

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“…DNA and total RNA from P. roqueforti were extracted as described by Gil-Durán et al (2015) . RT-PCR experiments were performed as described by García-Rico et al (2017) . Briefly, total RNA extracted was treated with RNase-free DNase I (Roche, Germany) and then quantified using a μDrop plate in a MultiSkan GO quantification system (Thermo Scientific, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Reactions contained 200 ng of treated RNA and 10 pmol of primers RiSfkF and RiSfkR. Amplification of β-tubulin cDNA (internal control) was performed as described by García-Rico et al (2017) . The products of the reactions were resolved by electrophoresis and quantified by densitometry analysis using the “myImageAnalysis” software (Thermo Scientific, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…The apical extension rates, the production of conidia, and the measurements of conidial germination were done exactly as described by Gil-Durán et al (2015) . The thermal and hypertonic stress analyses were performed as described by García-Rico et al (2017) .…”

Section: Methodsmentioning

confidence: 99%

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Role of sfk1 Gene in the Filamentous Fungus Penicillium roqueforti

et al. 2017

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The sfk1 (suppressor of four kinase) gene has been mainly studied in Saccharomyces cerevisiae, where it was shown to be involved in growth and thermal stress resistance. This gene is widely conserved within the phylum Ascomycota. Despite this, to date sfk1 has not been studied in any filamentous fungus. Previously, we found that the orthologous of sfk1 was differentially expressed in a strain of Penicillium roqueforti with an altered phenotype. In this work, we have performed a functional characterization of this gene by using RNAi-silencing technology. The silencing of sfk1 in P. roqueforti resulted in decreased apical growth and the promotion of conidial germination, but interesting, it had no effect on conidiation. In addition, the attenuation of the sfk1 expression sensitized the fungus to osmotic stress, but not to thermal stress. RNA-mediated gene-silencing of sfk1 also affected cell wall integrity in the fungus. Finally, the silencing of sfk1 depleted the production of the main secondary metabolites of P. roqueforti, namely roquefortine C, andrastin A, and mycophenolic acid. To the best of our knowledge this is the first study of the sfk1 gene in filamentous fungi.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Role of sfk1 Gene in the Filamentous Fungus Penicillium roqueforti

et al. 2017

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“…In most characterized filamentous fungi, the Gα proteins are classified as three groups (Gα1–3) (Li et al, 2007). Although the functions of individual Gα subunits have been well investigated in model and pathogenic fungi (Li et al, 2010; Yang et al, 2012; Hu et al, 2013; Wasil et al, 2013; Garcia-Rico et al, 2017), there is limited research on the interplay among different Gα subunits (Kamerewerd et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Effects of Different G-Protein α-Subunits on Growth, Development and Secondary Metabolism of Monascus ruber M7

et al. 2019

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Strains of Monascus filamentous fungal species have been used to produce fermented foods in Asian countries, such as China, Japan, and The Korean Peninsula, for nearly 2,000 years. At present, their fermented products are widely used as food additives and nutraceutical supplements worldwide owing to their production of beneficial secondary metabolites. Heterotrimeric G-protein signaling pathways participate in regulating multiple biological processes in fungi. Previously, we identified three Monascus ruber M7 G-protein α subunits (Mga1–3) and demonstrated that Mga1 can regulate growth, reproduction and some secondary metabolites’ production. Here, we systematically analyzed and compared the roles of mga 1–3 by combining single- and double-gene(s) knockouts and their transcriptomic data. First, mga 2 and mga 3 knock-out mutants and pairwise combinations of mga 1–3 deletion strains were generated. Then the changes in growth, development and the main secondary metabolites, Monascus pigments and citrinin, in these mutants were systematically compared with M. ruber M7. Moreover, RNA-Seq analyses of these mutants were performed. All three Gα subunits worked together to regulate biological processes in M. ruber M7, with Mga1 playing a major role, while Mga2 and Mga3 playing supplemental roles. According to the existing literatures which we can find, gene knock-out mutants of the pairwise combination of mga 1–3 and their transcriptome analysis are first reported in this study. The current results have clearly demonstrated the functional division of Mga1–3 in M. ruber M7, and could provide a deeper understanding of the effects of different Gα subunits on growth, development and secondary metabolism in other filamentous fungi.

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“…Heterotrimeric G protein consists of a, b and g subunit encoded by independent genes. It is widely reported in fungi such as Penicillium camemberti and Pestalotiopsis microspora that the Ga-cAMP signaling pathway controls growth, conidiation, development, secondary metabolism and stress response [6,7].…”

Section: Introductionmentioning

confidence: 99%

The heterotrimeric G protein г Stgg1 is required for conidiation, secondary metabolite production and pathogenicity of Setosphaeria turcica

Zhang

Lin³

et al. 2018

Biotechnology & Biotechnological Equipment

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Heterotrimeric G proteins are best known for their role in the transduction of extracellular signals to various downstream effectors. G proteins in higher eukaryotes are intensively studied; however, their roles in foliar pathogens are still elusive. In this study, we cloned the gene Stgg1 encoding G protein g subunit in Setosphaeria turcica and investigated its function by RNA interference technology. Three independent Stgg1 targeted RNAi mutants R3, R5 and R6 with diverse silencing efficiency were generated. Knock-down of Stgg1 resulted in a significant reduction in mRNA levels of the genes encoding Ga (Stga1, Stga2, Stga3) but not for Gb (Stgb1). Stgg1 RNAi mutants exhibited significantly elongated hyphal cells with blocked conidium production. In addition, Stgg1 RNAi mutants all appeared in lighter colony colour compatible with inhibited secondary metabolites. Further assays demonstrated that Stgg1 was required for biosynthesis of melanin and HT-toxin activity. Furthermore, down-regulation of Stgg1 largely inhibited the inflection capacity. Thus, we proposed that Stgg1 played crucial roles in conidiation, secondary metabolite production and pathogenicity of S. turcica and is, therefore, an ideal target for drug design against foliar pathogens.

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Heterotrimeric G protein alpha subunit controls growth, stress response, extracellular protease activity, and cyclopiazonic acid production in Penicillium camemberti

Cited by 17 publications

References 60 publications

Role of sfk1 Gene in the Filamentous Fungus Penicillium roqueforti

Role of sfk1 Gene in the Filamentous Fungus Penicillium roqueforti

Effects of Different G-Protein α-Subunits on Growth, Development and Secondary Metabolism of Monascus ruber M7

The heterotrimeric G protein г Stgg1 is required for conidiation, secondary metabolite production and pathogenicity of Setosphaeria turcica

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