Heterotrimeric G protein subunits differentially respond to endoplasmic reticulum stress in Arabidopsis

Cho, Yueh; Yu, Chao-Yuan; Iwasa, Takeshi; Kanehara, Kazue

doi:10.1080/15592324.2015.1061162

Cited by 14 publications

(18 citation statements)

References 24 publications

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“…b). These ER stress marker genes were all significantly upregulated at 5 h after Tm treatment in WT, as previously reported (Cho et al ., ). In cek1‐1 , the expression level of UPR genes was slightly, but significantly, higher than in WT, indicating that cek1‐1 is more susceptible to Tm treatment than WT.…”

Section: Resultsmentioning

confidence: 97%

Arabidopsis CHOLINE/ETHANOLAMINE KINASE 1 (CEK1) is a primary choline kinase localized at the endoplasmic reticulum (ER) and involved in ER stress tolerance

2019

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Choline kinase catalyzes the initial reaction step of choline metabolism that produces phosphocholine, a prerequisite for the biosynthesis of a primary phospholipid phosphatidylcholine. However, the primary choline kinase and its role in plant growth remained elusive in seed plants.Here, we showed that Arabidopsis CHOLINE/ETHANOLAMINE KINASE 1 (CEK1) encodes functional CEK that prefers choline than ethanolamine as a substrate in vitro and affects contents of choline and phosphocholine but not phosphatidylcholine in vivo.CEK1 is localized at endoplasmic reticulum (ER); upon tunicamycin-induced ER stress, a null mutant of CEK1 showed hypersensitive phenotype in seedlings, albeit with no enhanced choline kinase activity.Our results demonstrate that CEK1 is a primary ER-localized choline kinase in vivo that is required for ER stress tolerance possibly through the modulation of choline metabolites.

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Section: Resultsmentioning

confidence: 97%

Arabidopsis CHOLINE/ETHANOLAMINE KINASE 1 (CEK1) is a primary choline kinase localized at the endoplasmic reticulum (ER) and involved in ER stress tolerance

2019

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“…To observe the ER stress response in roots of intact plants, we employed Arabidopsis BiP3 gene as a reporter. BiP3 is a widely used marker gene for the ER stress response, whose expression is extremely low at either RNA or protein levels under non-stress conditions but is highly up-regulated upon the ER stress in Arabidopsis young seedlings ( Noh et al, 2003 ; Cho et al, 2015 ). We established a transgenic Arabidopsis plant that stably expresses BiP3-GUS fusion protein containing the ER retention signal (HDEL) for ER localization, which is driven by the own promoter ( ProBiP3:BiP3-GUS-HDEL in WT background).…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, high abundance in BiP3 proteins was detected in roots after 10 h of ER stress treatment ( Figure 3C ). Of note, no obvious morphological changes were found in roots during the short-term TM treatment ( Figure 4 and Supplementary Figure S2 ), while the long-term TM treatment is known to cause the root growth defect ( Cho et al, 2015 ; Kanehara et al, 2015b ).…”

Section: Discussionmentioning

confidence: 99%

“…In an effort to explore the ER stress response in the plant root system and address tissue-specific response in an intact multicellular organism, current study investigated ER stress response in Arabidopsis roots. To monitor the ER stress responses, a well-described UPR gene BiP3 has been employed because of the extremely low expression under non-stress condition but acute induction upon ER stress ( Noh et al, 2003 ; Srivastava et al, 2013 ; Cho et al, 2015 ). Based on the time-course observation of stable transgenic plant expressing the ProBiP3:BiP3-GUS-HDEL or ProBiP3:mRFP , we found that BiP3 was differentially expressed in root under the ER stress condition: the root tip including columella, outer layers in elongation zone and inner layers in mature zone were highly responsive to the ER stress.…”

Section: Introductionmentioning

confidence: 99%

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Endoplasmic Reticulum Stress Response in Arabidopsis Roots

Cho

Kanehara

2017

Front. Plant Sci.

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Roots are the frontier of plant body to perceive underground environmental change. Endoplasmic reticulum (ER) stress response represents circumvention of cellular stress caused by various environmental changes; however, a limited number of studies are available on the ER stress responses in roots. Here, we report the tunicamycin (TM) -induced ER stress response in Arabidopsis roots by monitoring expression patterns of immunoglobulin-binding protein 3 (BiP3), a representative marker for the response. Roots promptly responded to the TM-induced ER stress through the induction of similar sets of ER stress-responsive genes. However, not all cells responded uniformly to the TM-induced ER stress in roots, as BiP3 was highly expressed in root tips, an outer layer in elongation zone, and an inner layer in mature zone of roots. We suggest that ER stress response in roots has tissue specificity.

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“…Wang et al proposed that Arabidopsis agb1-2 mutation ameliorates leaf senescence under tunicamycin (Tm) treatment, possibly due to reduced expression levels of BiP3 and PDB transcripts (Wang et al, 2007 ). However, later studies provided contradicting evidence whereby agb1-1, agb1-2 , and agb1-3 all exhibited Tm-induced UPR-sensitive phenotype (Chen and Brandizzi, 2012 ; Cho et al, 2015 ) and higher BiP3 expression upon tunicamycin treatment. Gβ and IRE1A/1B seemed to mediate UPR independently from each other, since Gβ- and IRE1A/B-associated UPR signaling pathways additively contributed to ER stress sensitivity (Chen and Brandizzi, 2012 ).…”

Section: G Proteins and Abiotic Stress Response In Plantsmentioning

confidence: 99%

Genetic and Systematic Approaches Toward G Protein-Coupled Abiotic Stress Signaling in Plants

Urano

2018

Front. Plant Sci.

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Heterotrimeric G protein, composed of Gα, Gβ, and Gγ subunits, modulates plant adaptations to environmental stresses such as high salinity, drought, extreme temperatures and high light intensity. Most of these evidence were however derived solely from conventional genetics methods with which stress-associated phenotypes were compared between wild type and various G protein mutant plants. Recent advances in systematic approaches, mainly transcriptome and proteome, have contributed to in-depth understanding of molecular linkages between G proteins and environmental changes. Here, we update our knowledge on the roles of G proteins in abiotic stress responses. Furthermore, we highlight the current whole genome studies and integrated omics approach to better understand the fundamental G protein functions involved in abiotic stress responses. It is our purpose here to bridge the gap between molecular mechanisms in G protein science and stress biology and pave the way toward crop improvement researches in the future.

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Heterotrimeric G protein subunits differentially respond to endoplasmic reticulum stress in Arabidopsis

Cited by 14 publications

References 24 publications

Arabidopsis CHOLINE/ETHANOLAMINE KINASE 1 (CEK1) is a primary choline kinase localized at the endoplasmic reticulum (ER) and involved in ER stress tolerance

Arabidopsis CHOLINE/ETHANOLAMINE KINASE 1 (CEK1) is a primary choline kinase localized at the endoplasmic reticulum (ER) and involved in ER stress tolerance

Endoplasmic Reticulum Stress Response in Arabidopsis Roots

Genetic and Systematic Approaches Toward G Protein-Coupled Abiotic Stress Signaling in Plants

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