Heterotrophic cultivation of Chlorella vulgaris using broken rice hydrolysate as carbon source for biomass and pigment production

Cai, Yihui; Liu, Yuhuan; Liu, Tongying; Gao, Kaili; Zhang, Qi; Cao, Leipeng; Wang, Yunpu; Wu, Xiao‐Tao; Zheng, Hongli; Peng, Hong; Ruan, Roger

doi:10.1016/j.biortech.2020.124607

Cited by 22 publications

(12 citation statements)

References 32 publications

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“…On the other hand, less energy is required to utilize ammonium nitrogen [ 38 , 39 ]. Therefore, in this case, C. vulgaris will preferentially utilize ammonium nitrogen, and as the concentration of ammonium nitrogen decreases, C. vulgaris will begin to utilize nitrate nitrogen [ 25 ]. In this study, sucrase was used in the enzymatic hydrolysis of the sweet sorghum extract.…”

Section: Resultsmentioning

confidence: 99%

“…Yadav and Singh [ 40 ] found that a slightly alkaline pH (7.50–8.50) environment was optimal for the synthesis of proteins and carbohydrates by C. vulgaris . In addition, a weakly acidic pH (6.00) environment was conducive to the absorption and utilization of glucose in the medium by C. vulgaris [ 25 ]. Under the culture strategy of this study, the C. vulgaris efficiently utilized the glucose under acidic conditions in the early stage, and then entered a weak alkaline environment that was conducive to the synthesis of value-added chemicals.…”

Section: Resultsmentioning

confidence: 99%

“…Briefly, after centrifugation at 8000 rpm for 5 min at 4 °C, the microalgal cell pellets were collected and washed twice with distilled water, then dried at 80 °C for 24 h [ 24 ]. The growth kinetic parameters of microalgal cells were calculated according to the method of Cai et al [ 25 ]. The specific growth rate per day ( μ ) of the C. vulgaris was calculated according to Equation (1):

where W n and W n −1 are the dry cell weights on day n and day n − 1, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic Chlorella vulgaris Using Sweet Sorghum Extract

Fang

Hong³

et al. 2022

Foods

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High-cost carbon sources are not economical or sustainable for the heterotrophic culture of Chlorella vulgaris. In order to reduce the cost, this study used sweet sorghum extract (SE) and its enzymatic hydrolysate (HSE) as alternative carbon sources for the heterotrophic culture of Chlorella vulgaris. Under the premise of the same total carbon concentration, the value-added product production performance of Chlorella vulgaris cultured in HSE (supplemented with nitrogen sources and minerals) was much better than that in the glucose medium. The conversion rate of the total organic carbon and the utilization rate of the total nitrogen were both improved in the HSE system. The biomass production and productivity using HSE reached 2.51 g/L and 0.42 g/L/d, respectively. The production of proteins and lipids using HSE reached 1.17 and 0.35 g/L, respectively, and the production of chlorophyll-a, carotenoid, and lutein using HSE reached 30.42, 10.99, and 0.88 mg/L, respectively. The medium cost using HSE decreased by 69.61% compared to glucose. This study proves the feasibility and practicability of using HSE as a carbon source for the low-cost heterotrophic culture of Chlorella vulgaris.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

where W n and W n −1 are the dry cell weights on day n and day n − 1, respectively.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic Chlorella vulgaris Using Sweet Sorghum Extract

Fang

Hong³

et al. 2022

Foods

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“…The preparation of hydrolysate from broken rice was based on the method former reported [ 18 ]. Briefly, broken rice was gelatinized in boiling water for 30 min, and then hydrolyzed with 0.24% α-amylase (3,700 U/g, Solarbio Science and Technology Co., Ltd.) and 0.59% glucoamylase (10 5 U/g, Solarbio Science and Technology Co., Ltd.).…”

Section: Methodsmentioning

confidence: 99%

“…Broken rice is a by-product of rice processing, and its starch content is up to 75% [ 18 ]. As a major rice processing country, China’s broken rice produced during rice processing is conservatively estimated to be 30 million tons per year [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of C/N ratio on the growth and protein accumulation of heterotrophic Chlorella in broken rice hydrolysate

Cai

Zhai

Fang³

et al. 2022

Biotechnol Biofuels

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Background Microalgae protein is considered as a sustainable alternative to animal protein in the future. Using waste for microalgal culture can upgrade low-value raw materials into high-value products, helping to offset the cost of microalgal protein production. In this study we explored the feasibility of using microalgae heterotrophic fermentation to convert broken rice hydrolysate (BRH) into protein. Results The results showed that the increase of BRH supplemental ratio was beneficial to the increase of biomass production but not beneficial to the increase of intracellular protein content. To further improve protein production, the effect of C/N ratio on intracellular protein accumulation was studied. It was found that low C/N ratio was beneficial to the synthesis of glutamate in microalgae cells, which in turn promoted the anabolism of other amino acids and further the protein. When the C/N ratio was 12:1, the biomass productivity and protein content could reach a higher level, which were 0.90 g/L/day and 61.56%, respectively. The obtained Chlorella vulgaris biomass was rich in essential amino acids (41.80%), the essential amino acid index was as high as 89.07, and the lysine content could reach up to 4.05 g/100 g. Conclusions This study provides a theoretical basis and guidance for using Chlorella vulgaris as an industrial fermentation platform to convert broken rice into products with high nutritional value.

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Traditional Cultivation System

Prakash

Vasistha

2023

Microalgae Biotechnology for Wastewater Treatment, Resource Recovery and Biofuels

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Heterotrophic cultivation of Chlorella vulgaris using broken rice hydrolysate as carbon source for biomass and pigment production

Cited by 22 publications

References 32 publications

Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic Chlorella vulgaris Using Sweet Sorghum Extract

Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic Chlorella vulgaris Using Sweet Sorghum Extract

Effects of C/N ratio on the growth and protein accumulation of heterotrophic Chlorella in broken rice hydrolysate

Traditional Cultivation System

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