Heterotrophic cultivation of Ettlia sp. based on sequential hydrolysis of Helianthus tuberosus and algal residue

Kim, Minsik; Cho, Jun Muk; Kim, Hee Su; Lee, Hansol; Oh, Hee‐Mock; Chang, Yong Keun

doi:10.1016/j.enconman.2020.112769

Cited by 13 publications

(5 citation statements)

References 55 publications

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“…Moreover, the optimized conditions for obtaining the maximum biomass productivity in the heterotrophic cultivation of Ettlia sp. were recently established using sequential hydrolysis of Helianthus tuberosus and algal residue and successfully achieved 43.3 g L −1 on day 6 in the early stationary growth phase 11,13 . Thus, Pcrt can be very attractive as an inducible promoter to achieve high production of biomass, lipids, and recombinant proteins within a short period in the case of heterotrophic cultivation.…”

Section: Discussionmentioning

confidence: 99%

“…The optimized culture conditions for Ettlia sp. have also been determined to yield a maximized biomass productivity of 1.67 g L −1 d −1 and 7.21 g L −1 d −1 in autotrophic and heterotrophic cultivation, respectively 11 – 13 . Moreover, various carotenoids, such as lutein, β-carotene, violaxanthin, and astaxanthin, accumulate with growth and/or suitable abiotic stress 14 .…”

Section: Introductionmentioning

confidence: 99%

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Development of a species-specific transformation system using the novel endogenous promoter calreticulin from oleaginous microalgae Ettlia sp.

Lee

et al. 2020

Sci Rep

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Microalgae not only serve as raw materials for biofuel but also have uses in the food, pharmaceutical, and cosmetic industries. However, regulated gene expression in microalgae has only been achieved in a few strains due to the lack of genome information and unstable transformation. This study developed a species-specific transformation system for an oleaginous microalga, Ettlia sp. YC001, using electroporation. The electroporation was optimized using three parameters (waveform, field strength, and number of pulses), and the final selection was a 5 kV cm −1 field strength using an exponential decay wave with one pulse. A new strong endogenous promoter CRT (Pcrt) was identified using transcriptome and quantitative PCR analysis of highly expressed genes during the late exponential growth phase. The activities of this promoter were characterized using a codon optimized cyan fluorescent protein (CFP) as a reporter. The expression of CFP was similar under Pcrt and under the constitutive promoter psaD (PpsaD). The developed transformation system using electroporation with the endogenous promoter is simple to prepare, is easy to operate with high repetition, and utilizes a species-specific vector for high expression. This system could be used not only in molecular studies on microalgae but also in various industrial applications of microalgae.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a species-specific transformation system using the novel endogenous promoter calreticulin from oleaginous microalgae Ettlia sp.

Lee

et al. 2020

Sci Rep

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“…With less nutrient input, the microalgae could be well cultivated using the heterotrophic cultivation, but the alternate C and N source can be selected carefully. Kim et al 55 utilized hydrolysates of Helianthus tuberosus using sulfuric acid and algal residue to cultivate Ettlia sp. YC001.…”

Section: Heterotrophic Modementioning

confidence: 99%

“…Kim et al 55 utilized hydrolysates of Helianthus tuberosus using sulfuric acid and algal residue to cultivate Ettlia sp. YC001.…”

Section: Cultivation Conditions For Lipid Production From Microalgaementioning

confidence: 99%

Insights about sustainable biodiesel production from microalgae biomass: A review

et al. 2020

Intl J of Energy Research

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Production of biodiesel from microalgae can be categorized as a symbiotic engineering solution focusing on energy and environmental problems. This review presents a collection of recent methods adopted for the production of biodiesel from microalgae. Microalgae are tiny cell factories which yield a large quantity of biomass by employing different methods of cultivation and using different types of harvesting techniques. Different strategies like nutrient starvation and micronutrient supplementation for hyper lipid accumulation to produce biodiesel for competing with the high market demand for fossil fuel are discussed. The use of different cultivation techniques for increasing the algal biomass in the recent scenario is focused. Also, improvement in the design strategies of bioreactors like the open pond, photobioreactors, and hybrid systems is included in the review. Further, diverse technologies for lipid improvement, including manipulating the bioprocess parameters and genetic engineering, are addressed. Also, microalgae for biorefinery approaches solve pollution problems, and its biomass can be used for biodiesel production which is elaborated in detail. The possibility of production of biodiesel from microalgae at a larger scale by overcoming the main bottlenecks in different steps of algal growth like high biomass cultivation, investment factor, and implementation of biorefinery approaches to address the current energy demand is also proposed.

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“…In a recent investigation, the microalga Ettlia sp. was cultivated on Jerusalem artichoke and lipid-extracted microalgal biomass hydrolysate as carbon and nitrogen sources, respectively [48]. Unlike most microalgae, Ettlia sp.…”

Section: Fructose-rich Agricultural Residuesmentioning

confidence: 99%

The Prospects of Agricultural and Food Residue Hydrolysates for Sustainable Production of Algal Products

2020

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The growing demand of microalgal biomass for biofuels, nutraceuticals, cosmetics, animal feed, and other bioproducts has created a strong interest in developing low-cost sustainable cultivation media and methods. Agricultural and food residues represent low-cost abundant and renewable sources of organic carbon that can be valorized for the cultivation of microalgae, while converting them from an environmental liability to an industrial asset. Biochemical treatment of such residues results in the release of various sugars, primarily glucose, sucrose, fructose, arabinose, and xylose along with other nutrients, such as trace elements. These sugars and nutrients can be metabolized in the absence of light (heterotrophic) or the presence of light (mixotrophic) by a variety of microalgae species for biomass and bioproduct production. The present review provides an up-to-date critical assessment of the prospects of various types of agricultural and food residues to serve as algae feedstocks and the microalgae species that can be grown on such residues under a range of cultivation conditions. Utilization of these feedstocks can create potential industrial applications for sustainable production of microalgal biomass and bioproducts.

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Heterotrophic cultivation of Ettlia sp. based on sequential hydrolysis of Helianthus tuberosus and algal residue

Cited by 13 publications

References 55 publications

Development of a species-specific transformation system using the novel endogenous promoter calreticulin from oleaginous microalgae Ettlia sp.

Development of a species-specific transformation system using the novel endogenous promoter calreticulin from oleaginous microalgae Ettlia sp.

Insights about sustainable biodiesel production from microalgae biomass: A review

The Prospects of Agricultural and Food Residue Hydrolysates for Sustainable Production of Algal Products

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