Heterotrophic cultivation of Nannochloropsis salina for enhancing biomass and lipid production

Marudhupandi, Thangapandi; Sathishkumar, R; Kumar, T. T. Ajith

doi:10.1016/j.btre.2016.02.001

Cited by 49 publications

(25 citation statements)

References 46 publications

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“…Previous studies highlighted the ability of this alga to metabolize external carbon sources under photosynthetic conditions (mixotrophy) to improve growth 70 – 72 . We reproduced the reported growth enhancement when cells were shifted from phototrophy (without organic carbon) to a carbon-rich medium 73 , and found that changes in the trophic lifestyle of this alga were also accompanied by substantial physiological and morphological changes (Fig. 7a ).…”

Section: Resultssupporting

confidence: 83%

Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging

et al. 2021

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Eukaryotic phytoplankton have a small global biomass but play major roles in primary production and climate. Despite improved understanding of phytoplankton diversity and evolution, we largely ignore the cellular bases of their environmental plasticity. By comparative 3D morphometric analysis across seven distant phytoplankton taxa, we observe constant volume occupancy by the main organelles and preserved volumetric ratios between plastids and mitochondria. We hypothesise that phytoplankton subcellular topology is modulated by energy-management constraints. Consistent with this, shifting the diatom Phaeodactylum from low to high light enhances photosynthesis and respiration, increases cell-volume occupancy by mitochondria and the plastid CO2-fixing pyrenoid, and boosts plastid-mitochondria contacts. Changes in organelle architectures and interactions also accompany Nannochloropsis acclimation to different trophic lifestyles, along with respiratory and photosynthetic responses. By revealing evolutionarily-conserved topologies of energy-managing organelles, and their role in phytoplankton acclimation, this work deciphers phytoplankton responses at subcellular scales.

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Section: Resultssupporting

confidence: 83%

Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging

et al. 2021

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“…In addition, trials using other media or reinforcing BG11 medium by adding sodium acetate might be advantageous [37,38]. Moreover, addition of carbon-source supplements or sugars created by heterotrophic cultivation [39,40], may also help further in effective production. Use of genetically modified BX1.5 strains may also create an opportunity for increased production potential for the future since genes related to TAG production have been reported [7,31,33,41,42].…”

Section: Resultsmentioning

confidence: 99%

Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions

Sasaki

Takagi

Ota

et al. 2020

Biotechnology Reports

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A novel strain of microalga Parachlorella sp. BX1.5 was isolated and its unique properties of producing lipids and extracellular polysaccharides (EPS) characterized. The cells could extracellularly produce a large amount of acidic EPS, when cultured in nitrogen-deficient BG110 medium (BG11–N) with 2 % CO2-air supply. The main component of intracellularly accumulated lipids was triacylglycerol (TAG), depending on the different cultivation conditions of BG11, BG11–N, BG11–P (phosphate depleted), and BG11–N–P (nitrogen and phosphate depleted). Fatty-methyl-esters (FAMEs), methyl-esterification of total lipids, consisted of abundant saturated C16 and unsaturated C18 fatty acids under the culture conditions. Cell spot assays on BG11 plates revealed the resistance of cells to pH 2–11, high temperatures of 50–70 °C, ultraviolet irradiation, and drought, under different culture conditions, thereby suggesting the biological significance of lipid and EPS accumulation. The prospects of BX1.5 as a dual producer has also been discussed for biorefineries.

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“…The fatty acids EPA and DHA exert a range of biological activities in humans, some overlapping but others distinct for each fatty acid (Calder 2014 ). Fish oil, therefore, remains the main source of marine-derived EPA at present, as EPA-producing algal sources have largely been of photosynthetic origin although attempts have been made to culture some species heterotrophically and optimize conditions to enhance biomass and lipid contents (Marudhupandi et al 2016 ; Ratledge 2005 ). Nevertheless, a Schizochytrium strain/species with a minimum EPA and DHA content of 10 and 22% of total fatty acids, respectively, was reported previously (Gray 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Microbial and genetically engineered oils as replacements for fish oil in aquaculture feeds

2017

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As the global population grows more of our fish and seafood are being farmed. Fish are the main dietary source of the omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA), eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, but these cannot be produced in sufficient quantities as are now required for human health. Farmed fish have traditionally been fed a diet consisting of fishmeal and fish oil, rich in n-3 LC-PUFA. However, the increase in global aquaculture production has resulted in these finite and limited marine ingredients being replaced with sustainable alternatives of terrestrial origin that are devoid of n-3 LC-PUFA. Consequently, the nutritional value of the final product has been partially compromised with EPA and DHA levels both falling. Recent calls from the salmon industry for new sources of n-3 LC-PUFA have received significant commercial interest. Thus, this review explores the technologies being applied to produce de novo n-3 LC-PUFA sources, namely microalgae and genetically engineered oilseed crops, and how they may be used in aquafeeds to ensure that farmed fish remain a healthy component of the human diet.

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Heterotrophic cultivation of Nannochloropsis salina for enhancing biomass and lipid production

Abstract: Graphical abstract

Cited by 49 publications

References 46 publications

Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging

Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging

Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions

Microbial and genetically engineered oils as replacements for fish oil in aquaculture feeds

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