Heterotrophic sulfide-oxidizing nitrate-reducing bacteria enables the high performance of integrated autotrophic-heterotrophic denitrification (IAHD) process under high sulfide loading

Zhang, Ruochen; Chen, Chuan; Shao, Bo; Wang, Wei; Xu, Xiaomei; Zhou, Xu; Xiang, Yu-Nong; Zhao, Lei; Lee, Dong Hoon

doi:10.1016/j.watres.2020.115848

Cited by 67 publications

(33 citation statements)

References 44 publications

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“…Sulfide oxidation by heterotrophic denitrifiers has been previously reported in H. mongoliensis and H. kenyensis, as well as several other Halomonas species (Sorokin, 2003;Boltianskaia et al, 2007). In a continuous integrated autotrophic-heterotrophic denitrification bioreactor, H. desiderata was observed to dominate, showing the ability to simultaneously remove sulfide and nitrate (Zhang et al, 2020). Halomonas was also found to dominate; it could enhance sulfide denitrification removal on high-salinity wastewaters (Liu et al, 2016a).…”

Section: Widespread Presence Of Sulfur Oxidation Pathways In the Wholmentioning

confidence: 63%

“…Halomonas is one of the most abundant genera in the ocean and is commonly isolated, and its success may be attributed to its metabolic and physiological versatility. For instance, some Halomonas species could produce compatible solutes (ectoine, glycine betaine, and hydroxyectoine), biopolymers [exopolysaccharides (EPSs) and polyhydroxyalkanoate (PHA)], and biosurfactants (Cánovas et al, 2000;Amjres et al, 2011;Schwibbert et al, 2011;Kawata et al, 2014); degrade aromatic compounds (García et al, 2004); and oxidize arsenite (Lin et al, 2012;Hamamura et al, 2014), Fe(II), Mn(II) (Homann et al, 2009;Dong et al, 2014), and sulfide (Boltianskaia et al, 2007;Zhang et al, 2020); and are capable of denitrification (González-Domenech et al, 2010;Guo et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Aerobic denitrification has been widely applied in biological nitrogen removal systems due to their high growth rate and their excellent nitrate removal ability. Recently, heterotrophic sulfide-oxidizing nitrate-reducing bacteria, such as Pseudomonas C27 (Chen et al, 2013;Guo et al, 2019;Zhang et al, 2020), have attracted increasing attention. Sulfide oxidation of heterotrophic denitrifiers has also been observed in Halomonas mongoliensis and Halomonas kenyensis, which can oxidize sulfide in the presence of acetate as the carbon source and nitrate or nitrous oxide as the electron acceptor (Boltianskaia et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have shown that Halomonas spp. dominate in bioreactors and play important roles in the removal of sulfide and nitrate (Liu et al, 2016a;Zhang et al, 2020). However, their sulfur oxidation mechanisms are unclear.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Aerobic Denitrification and Heterotrophic Sulfur Oxidation in the Genus Halomonas Revealed by Six Novel Species Characterizations and Genome-Based Analysis

Wang

Shao

2021

Front. Microbiol.

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Bacteria of Halomonas are widely distributed in various environments and play a substantial role in the nutrient cycle. In this report, 14 strains capable of aerobic denitrification and heterotrophic sulfur oxidation were isolated from different habitats. Based on the phenotypic, genotypic, and chemotaxonomic analyses, these strains were considered to represent six novel species of the genus Halomonas, for which the names Halomonas zhangzhouensis sp. nov. type strain CXT3-11T ( = MCCC 1A11036T = KCTC 72087T), Halomonas aerodenitrificans sp. nov. CYD-9T ( = MCCC 1A11058T = KCTC 72088T), Halomonas sulfidoxydans sp. nov. CYN-1-2T ( = MCCC 1A11059T = KCTC 72089T), Halomonas ethanolica sp. nov. CYT3-1-1T ( = MCCC 1A11081T = KCTC 72090T), Halomonas sulfidivorans sp. nov. NLG_F1ET ( = MCCC 1A13718T = KCTC 72091T), and Halomonas tianxiuensis sp. nov. BC-M4-5T ( = MCCC 1A14433T = KCTC 72092T) are proposed. Intriguingly, they formed a unique group with 11 other species designated as the “H. desiderata group.” To better understand their featured metabolisms, genes involved in denitrification and sulfur oxidation were analyzed, along with 193 other available genomes of the whole genus. Consistently, complete denitrification pathways were confirmed in the “H. desiderata group,” in which napA, narG, nirS, norB, and nosZ genes coexist. Their nitrite reductase NirS formed a unique evolutionary lineage, distinguished from other denitrifiers in Halomonas. In addition, diverse occurrence patterns of denitrification genes were also observed in different phylogenetic clades of Halomonas. With respect to sulfur oxidation, fccAB genes involved in sulfide oxidation commonly exist in the “H. desiderata group,” while sqr genes are diverse and can be found in more species; sqr genes co-occurred with fccAB in eight strains of this study, contributing to more active sulfide oxidation. Besides, the tsdA gene, which encodes an enzyme that oxidizes thiosulfate to tetrathionate, is ubiquitous in the genus Halomonas. The widespread presence of sqr/fccAB, pdo, and tsdA in Halomonas suggests that many Halomonas spp. can act as heterotrophic sulfur oxidizers. These results provide comprehensive insights into the potential of denitrification and sulfur oxidation in the whole genus of Halomonas. With regard to the global distribution of Halomonas, this report implies their unneglectable role in the biogeochemical cycle.

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Section: Widespread Presence Of Sulfur Oxidation Pathways In the Wholmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Aerobic Denitrification and Heterotrophic Sulfur Oxidation in the Genus Halomonas Revealed by Six Novel Species Characterizations and Genome-Based Analysis

Wang

Shao

2021

Front. Microbiol.

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“…The nitrogen removal abilities, especially denitrification of strains from genus Halomonas have been reported in previous works, such as H. campisalis ha3 [11] and Halomonas cerina 15CR [23]. Several studies have shown that Halomonas was dominant in bioreactors and contributed to satisfactory nitrogen and carbon removal efficiencies [24,25]. However, the denitrification ability of Halomonas piezotolerans has not been reported.…”

Section: Introductionmentioning

confidence: 93%

Characteristics and mechanism of heterotrophic nitrification/aerobic denitrification in a novel Halomonas piezotolerans strain

Dong

et al. 2021

J Basic Microbiol

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A strain was isolated from an activated sludge system and identified as Halomonas piezotolerans HN2 in this study, which is the first strain in H. piezotolerans with the capability of heterotrophic nitrification and aerobic denitrification. Strain HN2 showed the maximum nitrogen removal rate of 9.10 mg/L/h by utilizing ammonium at the salinity of 3.0%. Under saline environment, HN2 could remove nitrogen efficiently in neutral and slightly alkaline environments, with the carbon sources of sodium succinate and sodium citrate and the C/N ratio of 15-20, and the maximum removal efficiencies of ammonium, nitrite, and nitrate were 100%, 96.35%, and 99.7%, respectively.The genomic information revealed the presence of amoA, napA, and nosZ genes in strain HN2, and the target bands of nirS were obtained via a polymerase chain reaction. Therefore, we inferred that ammonium was mainly utilized for the growth of strain HN2 through assimilation, and another part of the initial ammonium was converted into nitrate through nitrification, and then into gaseous nitrogen through denitrification. This report indicated the potential application of strain HN2 and other nitrifying and denitrifying Halomonas strains in the removal of nitrogen pollution in marine-related environments and also implies the important role of Halomonas in the nitrogen cycle process of the ocean.

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Bidirectionally Regulating Viral and Cellular Ferroptosis with Metastable Iron Sulfide Against Influenza Virus

et al. 2023

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Influenza virus with numerous subtypes and frequent variation limits the development of high-efficacy and broad-spectrum antiviral strategy. Here, a novel multi-antiviral metastable iron sulfides (mFeS) against various influenza A/B subtype viruses is developed. This work finds that mFeS induces high levels of lipid peroxidation and •OH free radicals in the conservative viral envelope, which depends on Fe 2+ . This phenomenon, termed as a viral ferroptosis, results in the loss of viral infectibility and pathogenicity in vitro and in vivo, respectively. Furthermore, the decoction of mFeS (Dc(mFeS)) inhibits cellular ferroptosis-dependent intracellular viral replication by correcting the virus-induced reprogrammed sulfur metabolism, a conserved cellular metabolism. Notably, personal protective equipment (PPE) that is loaded with mFeS provides good antiviral protection. Aerosol administration of mFeS combined with the decoction (mFeS&Dc) has a potential therapeutic effect against H1N1 lethal infection in mice. Collectively, mFeS represents an antiviral alternative with broad-spectrum activity against intracellular and extracellular influenza virus.

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Heterotrophic sulfide-oxidizing nitrate-reducing bacteria enables the high performance of integrated autotrophic-heterotrophic denitrification (IAHD) process under high sulfide loading

Cited by 67 publications

References 44 publications

Aerobic Denitrification and Heterotrophic Sulfur Oxidation in the Genus Halomonas Revealed by Six Novel Species Characterizations and Genome-Based Analysis

Aerobic Denitrification and Heterotrophic Sulfur Oxidation in the Genus Halomonas Revealed by Six Novel Species Characterizations and Genome-Based Analysis

Characteristics and mechanism of heterotrophic nitrification/aerobic denitrification in a novel Halomonas piezotolerans strain

Bidirectionally Regulating Viral and Cellular Ferroptosis with Metastable Iron Sulfide Against Influenza Virus

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