The present study reveals the enhanced bio-sorption of methylene blue (MB) from an aqueous solution using iron oxide immobilized citrus bergamia biochar (Fe 3 O 4 CBC). The adsorption efficiency was increased dramatically from 64 % to 98 % after the magnetic iron oxide modification to citrus bergamia biochar (CBC) due to electrostatic interaction. Physico-chemical alterations of CBC surface prior and post-modification and after dye adsorption were evaluated using the following surface techniques such as FTIR, XRD, SEM, BET, Raman, TGA-DTA, Zeta potential analysis. The retention of CÀ OH, C=O, CÀ O, CÀ H, and Fe 3 O 4 groups were confirmed from FTIR spectra. BET analysis revealed that Fe 3 O 4 CBC showed an elevated surface area of 136.72 m 2 g À 1 with a reduced pore diameter of 3.73 nm than CBC. The Pseudo second order and Langmuir isotherm model was best fitted with R 2 = 0.99 and adsorption capacity of 31 mg/g. The thermodynamic data (ΔS = 0.1455 kJ mol À 1 K À 1 , ΔH = 33.87 kJ mol À 1 ) revealed the spontaneity, endothermic nature, and feasibility of adsorption process. Regeneration studies revealed that the material has excellent sorption-based performance over five consecutive cycles. The current work demonstrates that Fe 3 O 4 CBC can serve as a highly efficient, cost-effective, bio adsorbent for the removal of MB with easy magnetic separation from aqueous medium.