Hevea Latex: Effect of Proteins and Electrolytes on Colloidal Behavior.

Kemp, A. R.; Straitiff, W. G.

doi:10.1021/j150402a010

Cited by 13 publications

(13 citation statements)

References 4 publications

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“…Zeta potential analysis gives an indication on surface charge behaviour on rubber particle surface, thus distinct chemical nature of the absorbed layers on the surface of rubber particle is reflected in the difference in the zeta potential value consequently the colloidal stability of natural rubber latex. Adsorbed proteins on the surface of latex particle are reported to contain both acidic and alkaline functional groups that carry positive and negative charge respectively [20]. HA-NRL is composed of phospholipids, carbohydrates, proteins and other non-rubber constituents which contribute charge species that gives high negative zeta potential value [3].…”

Section: Results and Discussion Protein Contents And Zeta Potentialsmentioning

confidence: 99%

“…HA-NRL is composed of phospholipids, carbohydrates, proteins and other non-rubber constituents which contribute charge species that gives high negative zeta potential value [3]. Perturbation of the proteins layer by urea might compensate the surface charge hence compressed the diffuse layer and subsequently reduced the negative zeta potential value [20].…”

Section: Results and Discussion Protein Contents And Zeta Potentialsmentioning

confidence: 99%

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Zeta Potential and Environmental Scanning Electron Microscopy Analysis in Deproteinisation of Natural Rubber Latex

Abdullah¹

2016

MJAS

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This paper describes the effect of protein denaturants namely, urea and sodium dodecyl sulphate (SDS) in the deproteinisation of high ammonia natural rubber latex (HA-NRL). It had been shown that latex proteins were reduced more in the presence of urea alone (48%) than in the presence of SDS (18%). The synergistic effect when both urea and SDS were used in the latex deproteinisation attributed to the higher protein removal efficacy (55%). Higher zeta potential value was observed when only SDS was used, suggesting higher colloidal stability. Environmental Scanning Electron Microscopy (ESEM) revealed that without SDS, the use of urea single-handedly in the deproteinisation process promoted aggregation of latex particles, possibly due to the dismissal of latex proteins which helped to stabilise latex colloidal. Thus, it is most likely that urea serves as a protein unfolding agent during latex deproteinisation process, while SDS functions as a latex stabiliser with no significant effects on latex proteins discharge.Keywords: urea, sodium dodecyl sulphate, deproteinisation, zeta potential, environmental scanning electron microscopy Abstrak Kertas kerja ini menghuraikan kesan bahan-bahan pengubah sifat protein iaitu urea dan natrium dodekil sulfat (NDS) dalam penyahprotein lateks getah asli tinggi ammonia (HA-NRL). Ia telah menunjukkan bahawa protein lateks lebih banyak dikurangkan dengan kehadiran urea sahaja (48%) berbanding dengan kehadiran NDS sahaja (18%). Keberkesanan penyingkiran protein yang lebih tinggi (55%) mungkin telah disebabkan oleh kesan sinergi apabila kedua-dua urea dan NDS digunakan di dalam penyahprotein lateks. Nilai keupayaan zeta yang tinggi diperhatikan apabila hanya NDS digunakan, menunjukkan kestabilan koloid yang lebih tinggi. Pengimbas Persekitaran Mikroskopi Elektron (ESEM) menunjukkan bahawa tanpa NDS, penggunaan hanya urea dalam proses penyahprotein menyumbang kepada kesan pengagregatan zarah lateks, kemungkinan disebabkan oleh penyingkiran protein lateks yang membantu dalam kestabilan koloid lateks. Oleh yang demikian, besar kemungkinan fungsi utama urea semasa proses penyahprotein lateks adalah sebagai ejen pembuka protein manakala NDS berfungsi sebagai penstabil lateks tanpa kesan ketara pada penyingkiran protein lateks.

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Section: Results and Discussion Protein Contents And Zeta Potentialsmentioning

confidence: 99%

Section: Results and Discussion Protein Contents And Zeta Potentialsmentioning

confidence: 99%

Zeta Potential and Environmental Scanning Electron Microscopy Analysis in Deproteinisation of Natural Rubber Latex

Abdullah¹

2016

MJAS

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“…The present paper describes investigations on the proteins of fresh latex serum. Before 1942 the presence of three protein fractions in fresh serum had been demonstrated (Bishop, 1927;Kemp & Straitiff, 1940). Similar fractions were also obtained from ammonia-preserved latex, though the presence of ammonia modified the properties of the proteins in some respects (Kemp & Straitiff, 1940;Bondy & Freundlich, 1938).…”

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confidence: 87%

“…Before 1942 the presence of three protein fractions in fresh serum had been demonstrated (Bishop, 1927;Kemp & Straitiff, 1940). Similar fractions were also obtained from ammonia-preserved latex, though the presence of ammonia modified the properties of the proteins in some respects (Kemp & Straitiff, 1940;Bondy & Freundlich, 1938). Later, a more detailed electrophoretic analysis of the proteins in unammoniated serum was carried out by Roe & Ewart (1942), who demonstrated the presence of seven electrophoretically distinct protein constituents.…”

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confidence: 92%

The proteins of Hevea brasiliensis latex. 1. Protein constituents of fresh latex serum

Archer

Sekhar

1955

Biochemical Journal

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GLUCOSE IN PROTEIN SYNTHESIS 503 apparatus. However, an immunological method showed the presence of three additional minor components. 2. An antiserum, prepared by injection of chick serum albumin into rabbits, was used to demonstrate the net synthesis of serum albumin by chick-liver slices. The results of Peters & Anfinsen (1950) were thus confirmed. 3. The incorporation of radioactivity into serum albumin and a soluble protein fraction was studied after incubation of chick-liver slices with [14C]glucose and [14C]alanine respectively. The specific radioactivity of the alanine in each protein fraction was determined. 4. The incorporation of radioactivity into a soluble protein fraction of rat hepatoma and rat liver was studied after incubation of the tissue slices with [14C]glucose. The specific radioactivity of the glucose during the incubation period was followed. It was thus shown that under these conditions there is a better utilization of glucose by hepatoma tissue for the synthesis of protein than there is by liver tissue. I would like to thank Professor Dickens for his interest and encouragement throughout the course of the work, the British Empire Cancer Campaign for a grant to the Medical School covering the cost of the work, Mr H. Jones for help with the laparotomies, Dr J. H. Humphrey for advice on the preparation of the antiserum, Miss A. Doughty for the electrophoretic analyses, and Miss B. Kernot for technical assistance.

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“…Thus, the final stability of the blend will be determined by the degree of interaction between either the soap and inorganic salts present in the synthetic system with proteins, or their degradation products, and other ammonium salts which contribute to the stability of natural latcx (1,8). Thus, the final stability of the blend will be determined by the degree of interaction between either the soap and inorganic salts present in the synthetic system with proteins, or their degradation products, and other ammonium salts which contribute to the stability of natural latcx (1,8).…”

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confidence: 99%