Hexarelin, a novel GHRP-6 analog, stimulates growth hormone (GH) release in a GH-secreting rat cell line (GH1) insensitive to GH-releasing hormone

Effects of AMPK activation and combined treatment with AMPK activators and somatostatin on hormone secretion and cell growth in cultured GH-secreting pituitary tumor cells

“…Remarkable differences may exist between rat pituitary tumor cell lines and human primary tumors in the GHRH receptor (GHRH-R)-cAMP signalling pathways. Indeed, GH3 cells do not express GHRH receptor on cell surface and GH1 cells have been shown to be unresponsive to GHRH (Giustina et al, 1997;Lee et al, 2001). In turn, about 40% of patients with sporadic acromegaly harbour guanine nucleotide-binding protein alpha stimulating (GNAS) gene mutations, leading to constitutively elevated cAMP (Melmed, 2009).…”

Ghrelin: a hormone regulating food intake and energy homeostasis

“…Since the 1970s it has been known that a number of small synthetic molecules termed GHS act on the pituitary gland and the hypothalamus to stimulate and amplify pulsatile GH release (Bowers et al 1977) and this phenomenon was later shown independent of GHRH (Giustina et al 1997). This was followed by the development of a number of both peptide and non-peptide GHS (Bercu & Walker, 1997).…”

Site-Specific PEGylation for High-Yield Preparation of Lys21-Amine PEGylated Growth Hormone-Releasing Factor (GRF) (1−29) using a GRF(1−29) Derivative FMOC-Protected at Tyr1 and Lys12

“…In Vitro GH Releasing Activity in Rat Pituitary Cells. Monolayer cultures of anterior pituitary cells obtained from Wistar rats (150−200 g) were performed using a modification of a previously described method ( − ). Pituitary cells obtained were washed twice with 1 mL of supplemented DMEM and incubated at 37 °C for 30 min.…”