Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus

Shukor, Mohd Yunus; Rahman, M. Affan Abdul; Suhaili, Zarizal; Shamaan, Nor Aripin; Syed, Mohd Arif

doi:10.1007/s12223-010-0021-x

Cited by 47 publications

(67 citation statements)

References 32 publications

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“…Based on previous studies, the phosphate concentration required is around 5 mM and lower for optimal Mo-reduction [13,15,18,23,28,[30][31][32][33][34][35][36][37]39]. The result of the present study showed that maximum concentration of phosphate also occurred at 5.0 mM.…”

Section: Effect Of Phosphate and Molybdate Concentration On Moreductionsupporting

confidence: 58%

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Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Sabullah

Rahman

Ahmad

et al. 2017

J. Math. Fund. Sci.

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Abstract. One of the most economical approaches for removal of toxic compounds is bioremediation. In the long term, bioremediation is economic and feasible compared to other methods, such as physical or chemical methods. A bacterium that can efficiently reduce molybdenum blue was isolated from polluted soil. Biochemical analysis revealed the identity of the bacterium as Enterobacter sp. strain Saw-1. The growth parameters for optimal reduction of molybdenum to Mo-blue or molybdenum blue, a less toxic product, were determined around pH 6.0 to 6.5 and in the range of 30 to 37 ℃, respectively. Glucose was selected as preferred carbon source, followed by sucrose, maltose, l-rhamnose, cellobiose, melibiose, raffinose, d-mannose, lactose, glycerol, dadonitol, d-mannitol, l-arabinose and mucate. Phosphate and molybdate were critically required at 5.0 mM and 10 mM, respectively. The scanning absorption spectrum acquired to detect the development of complex Mo-blue showed similarity to previously isolated Mo-reducing bacteria. In addition, the spectrum closely resembled the molybdenum blue from the phosphate determination method. Heavy metals, including mercury, copper (II) and silver (I), inhibited reduction. Moreover, the bacterium also showed capability of exploiting the pesticide coumaphos as an alternative carbon source for growth. As the bacterium proved its ability to detoxify organic and inorganic xenobiotics, the usefulness of this microorganism for bioremediation is highlighted.

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Section: Effect Of Phosphate and Molybdate Concentration On Moreductionsupporting

confidence: 58%

“…strain Dr.Y2 [36], S. marcescens strain Dr.Y6 [29], Serratia sp. strain Dr.Y5 [37], Enterobacter sp. strain Dr.Y13 [18], Acinetobacter calcoaceticus [37], Bacillus sp.…”

Section: Effect Of Phosphate and Molybdate Concentration On Moreductionmentioning

confidence: 99%

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Sabullah

Rahman

Ahmad

et al. 2017

J. Math. Fund. Sci.

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“…The determination of phosphate and molybdate concentrations supporting optimal molybdenum reduction is important because both anions have been shown to inhibit Mo-blue production in bacteria (Shukor et al, 2008a;Shukor et al, 2009a;Yunus et al, 2009;Shukor et al, 2009b;Shukor et al, 2010a;Shukor et al, 2010b;Lim et al, 2012;Ahmad et al, 2013;Othman et al, 2013;. The optimum concentration of phosphate occurred at 5 mM with higher concentrations were strongly inhibitory to reduction (Figure 1d).…”

Section: Effect Of Phosphate and Molybdate Concentrations To Molybdatmentioning

confidence: 97%

“…strain Dr.Y5 (Rahman et al, 2009), Enterobacter sp. strain Dr.Y13 (Shukor et al, 2009a), Acinetobacter calcoaceticus strain Dr.Y12 (Shukor et al, 2010b), prefer glucose as the electron donor, while Enterobacter cloacae strain 48 (Ghani et al, 1993), Serratia sp. strain Dr.Y5 (Rahman et al, 2009), S. marcescens strain Dr.Y9 (Yunus et al, 2009) and S. marcescens strain DRY6 (Shukor et al, 2008a) prefer sucrose as the best electron donor.…”

Section: Mo-blue Production Using Various Electron Donorsmentioning

confidence: 99%

“…strain Dr.Y5 (Rahman et al, 2009), Enterobacter sp. strain Dr.Y13 (Shukor et al, 2009a) and Acinetobacter calcoaceticus (Shukor et al, 2010b) could produce optimal Mo-blue using the optimal molybdate concentrations at 50, 25, 55, 30, 30, 50 and 20 mM, respectively. In fact the highest concentration of molybdenum as a pollutant in the environment is around 2000 ppm or about 20 mM (Runnells et al, 1976).…”

Section: Effect Of Phosphate and Molybdate Concentrations To Molybdatmentioning

confidence: 99%

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ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND GLYPHOSATE-DEGRADING Klebsiella oxytoca STRAIN SAW-5 IN SOILS FROM SARAWAK

et al. 2016

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Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34 o C. Glucose was the best electron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spectrum resembling a reduced phosphomolybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants.

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Reduction of molybdate to molybdenum blue by Klebsiella sp. strain hkeem

Lim

Syed

Shukor

2011

J. Basic Microbiol.

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A novel molybdate-reducing bacterium, tentatively identified as Klebsiella sp. strain hkeem and based on partial 16s rDNA gene sequencing and phylogenetic analysis, has been isolated. Strain hkeem produced 3 times more molybdenum blue than Serratia sp. strain Dr.Y8; the most potent Mo-reducing bacterium isolated to date. Molybdate was optimally reduced to molybdenum blue using 4.5 mM phosphate, 80 mM molybdate and using 1% (w/v) fructose as a carbon source. Molybdate reduction was optimum at 30 °C and at pH 7.3. The molybdenum blue produced from cellular reduction exhibited absorption spectrum with a maximum peak at 865 nm and a shoulder at 700 nm. Inhibitors of electron transport system such as antimycin A, rotenone, sodium azide, and potassium cyanide did not inhibit the molybdenum-reducing enzyme. Mercury, silver, and copper at 1 ppm inhibited molybdenum blue formation in whole cells of strain hkeem.

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Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus

Cited by 47 publications

References 32 publications

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND GLYPHOSATE-DEGRADING Klebsiella oxytoca STRAIN SAW-5 IN SOILS FROM SARAWAK

Reduction of molybdate to molybdenum blue by Klebsiella sp. strain hkeem

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