HEXOKINASE1 signalling promotes shoot branching and interacts with cytokinin and strigolactone pathways

Barbier, François; Cao, Da; Fichtner, Franziska; Weiste, Christoph; Pérez-Garcia, Maria-Dolores; Caradeuc, Mathieu; Gourrierec, José Le; Sakr, Soulaïman; Beveridge, Christine A.

doi:10.1111/nph.17427

Cited by 66 publications

(69 citation statements)

References 102 publications

(178 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have shown that HXKs play an important role in different stages of plant development [35]. The mechanism by which HXK affects all stages of the plant life cycle may lie in the regulation of glucose abundance signals in several hormone signaling pathways, such as the auxin (AUX) [46], cytokinin (CK) [47], abscisic acid (ABA) [44], gibberellic acid (GA) [48], and brassinosteroid (BR) [49] pathways and even the growth regulator melatonin (MT) pathway [35]. Overexpression of OsHXK5, OsHXK6, or their noncatalytic mutant alleles in high-glucose media can restore the glucose-sensitive seedling development-arrested phenotype in Arabidopsis gin2-1 plants.…”

Section: Introductionmentioning

confidence: 99%

Improving the Rice Photosynthetic Efficiency and Yield by Editing OsHXK1 via CRISPR/Cas9 System

Zheng

et al. 2021

IJMS

View full text Add to dashboard Cite

Rice (Oryza sativa L.) is an important food crop species in China. Cultivating high-yielding rice varieties that have a high photosynthetic efficiency is an important goal of rice breeding in China. In recent years, due to the continual innovation of molecular breeding methods, many excellent genes have been applied in rice breeding, which is highly important for increasing rice yields. In this paper, the hexokinase gene OsHXK1 was knocked out via the CRISPR/Cas9 gene-editing method in the indica rice varieties Huanghuazhan, Meixiangzhan, and Wushansimiao, and OsHXK1-CRISPR/Cas9 lines were obtained. According to the results of a phenotypic analysis and agronomic trait statistics, the OsHXK1-CRISPR/Cas9 plants presented increased light saturation points, stomatal conductance, light tolerance, photosynthetic products, and rice yields. Moreover, transcriptome analysis showed that the expression of photosynthesis-related genes significantly increased. Taken together, our results revealed that knocking out OsHXK1 via the CRISPR/Cas9 gene-editing method could effectively lead to the cultivation of high-photosynthetic efficiency and high-yielding rice varieties. They also revealed the important roles of OsHXK1 in the regulation of rice yield and photosynthesis.

show abstract

Section: Introductionmentioning

confidence: 99%

Improving the Rice Photosynthetic Efficiency and Yield by Editing OsHXK1 via CRISPR/Cas9 System

Zheng

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Apart from SnRK1 and TOR, hexokinase 1 (HXK1) over-expressing plants showed loss of apical dominance [436]. It was confirmed in rose, pea and Arabidopsis that HXK1 promotes shoot branching [437]. These findings show that sugar signaling events control branching of vegetative shoots and inflorescences.…”

Section: Developmental Regulation By Sugar Signalingmentioning

confidence: 85%

Sweet Modifications Modulate Plant Development

et al. 2021

View full text Add to dashboard Cite

Plant development represents a continuous process in which the plant undergoes morphological, (epi)genetic and metabolic changes. Starting from pollination, seed maturation and germination, the plant continues to grow and develops specialized organs to survive, thrive and generate offspring. The development of plants and the interplay with its environment are highly linked to glycosylation of proteins and lipids as well as metabolism and signaling of sugars. Although the involvement of these protein modifications and sugars is well-studied, there is still a long road ahead to profoundly comprehend their nature, significance, importance for plant development and the interplay with stress responses. This review, approached from the plants’ perspective, aims to focus on some key findings highlighting the importance of glycosylation and sugar signaling for plant development.

show abstract

“…Sugars not only provided energy but also acted as a signal for bud outgrowth [ 50 , 51 ]. CTKs up-regulated the genes responsible for the sugar sink strength in previous studies.…”

Section: Discussionmentioning

confidence: 99%

Cyclanilide Induces Lateral Bud Outgrowth by Modulating Cytokinin Biosynthesis and Signalling Pathways in Apple Identified via Transcriptome Analysis

Xie

Zhao

et al. 2022

IJMS

View full text Add to dashboard Cite

Cyclanilide (CYC), a plant growth regulator, is a potent shoot branching agent in apple. However, its mechanism remains unclear. The current study revealed that CYC treatment resulted in massive reprogramming of the axillary bud transcriptome, implicating several hormones in the response. We observed a marked increase (approximately 2-fold) in the level of zeatin riboside and a significant decrease (approximately 2-fold) in the level of abscisic acid (ABA). Zeatin metabolism gene cytokinin (CTK) oxidase 1 (CKX 1) was down-regulated at 168 h after CYC treatment compared with the control. Weighted gene co-expression network analysis of differentially expressed genes demonstrated the turquoise module clusters exhibited the highest positive correlation with zeatin riboside (r = 0.92) and the highest negative correlation with ABA (r = −0.8). A total of 37 genes were significantly enriched in the plant hormone signal transduction pathway in the turquoise module. Among them, the expressions of CTK receptor genes WOODEN LEG and the CTK type-A response regulators genes ARR3 and ARR9 were up-regulated. ABA signal response genes protein phosphatase 2C genes ABI2 and ABI5 were down-regulated in lateral buds after CYC treatment at 168 h. In addition, exogenous application of 6-benzylaminopurine (6-BA, a synthetic type of CTK) and CYC enhanced the inducing effect of CYC, whereas exogenous application of lovastatin (a synthetic type of inhibitor of CTK biosynthesis) or ABA and CYC weakened the promoting effect of CYC. These results collectively revealed that the stimulation of bud growth by CYC might involve CTK biosynthesis and signalling, including genes CKX1 and ARR3/9, which provided a direction for further study of the branching promoting mechanism of CYC.

show abstract

HEXOKINASE1 signalling promotes shoot branching and interacts with cytokinin and strigolactone pathways

Cited by 66 publications

References 102 publications

Improving the Rice Photosynthetic Efficiency and Yield by Editing OsHXK1 via CRISPR/Cas9 System

Improving the Rice Photosynthetic Efficiency and Yield by Editing OsHXK1 via CRISPR/Cas9 System

Sweet Modifications Modulate Plant Development

Cyclanilide Induces Lateral Bud Outgrowth by Modulating Cytokinin Biosynthesis and Signalling Pathways in Apple Identified via Transcriptome Analysis

Contact Info

Product

Resources

About