HgCl2 disrupts the structure of the human erythrocyte membrane and model phospholipid bilayers

Suwalsky, Mario; Ungerer, Brigitte; Villena, Fernando Pardo-Manuel de; Cuevas, Francisco; Sotomayor, Carlos P.

doi:10.1016/s0162-0134(00)00105-7

Cited by 34 publications

(39 citation statements)

References 28 publications

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“…In addition, isolated resealed human erythrocyte membranes were analyzed by fluorescence spectroscopy, and perturbations of erythrocytes in the absence and presence of Hg 2+ and Cd 2+ were observed by scanning electron microscopy. Human erythrocytes incubated with 1 mM HgCl 2 exhibited both echinocyte and stomatocyte formation (Suwalsky et al, 2000) whereas 1 mM CdCl 2 only induced echinocytes (Suwalsky et al, 2004). According to the bilayer couple hypothesis, the shape induced in erythrocytes in the presence of heavy metals is due to the expansion of both monolayers in the membrane (Sheetz and Singer, 1974).…”

Section: Heavy Metals and Neurotoxic Cations: Multilamellar Vesiclesmentioning

confidence: 99%

“…Vesicles encapsulated with a fluorophore and planar lipid bilayers can also be used to look at metal and drug permeability. Generally, lipid model systems usually lack proteins, making them less fluid than biological membranes (Suwalsky et al, 2000). However, numerous studies have employed single, binary and ternary lipid mixtures in protein-free models to study ion, heavy metal, drug and peptide interactions.…”

Section: The Importance Of Lipid Model Systemsmentioning

confidence: 99%

“…The binary mixtures were shown to be in one phase in pure water and two distinct lamellar phases in 30 mM CaCl 2 using X-ray diffraction (Lis et al, 1981). Single-lipid containing MLVs, composed of DMPC or dimyristoyl-PE (DMPE), were used to study Zn 2+ -membrane interactions (Suwalsky et al, 1996). Zn 2+ was shown to interact with DMPE and DMPC bilayers using X-ray diffraction at a concentration as low as 10 -5 μM.…”

Section: I M Y R I S T O Y L P H O S P H a T I D I C A C I D ( D M mentioning

confidence: 99%

“…MLVs (DMPC and DMPE) were used with X-ray diffraction and LUVs (DMPC) with fluorescence spectroscopy to study the molecular mechanism of Hg 2+ and Cd 2+ interactions with the membrane (Suwalsky et al, 2000;Suwalsky et al, 2004). In addition, isolated resealed human erythrocyte membranes were analyzed by fluorescence spectroscopy, and perturbations of erythrocytes in the absence and presence of Hg 2+ and Cd 2+ were observed by scanning electron microscopy.…”

Section: Heavy Metals and Neurotoxic Cations: Multilamellar Vesiclesmentioning

confidence: 99%

“…A greater effect was observed with DMPC MLVs. The presence of Hg 2+ and Cd 2+ results in molecular disorder in the bilayer, affecting both the polar and acyl chain regions (Suwalsky et al, 2000;Suwalsky et al, 2004). The effect of HgCl 2 at the membrane surface was investigated using DPH steady state fluorescence anisotropy to look at lipid acyl chain packing and Laurdan fluorescence spectral shifts via general polarization to observe interactions occurring in the phospholipid glycerol backbone (Suwalsky et al, 2000;Suwalsky et al, 2004).…”

Section: Heavy Metals and Neurotoxic Cations: Multilamellar Vesiclesmentioning

confidence: 99%

See 4 more Smart Citations

Biomimetic Model Membrane Systems Serve as Increasingly Valuable in Vitro Tools

Le¹,

Litzenberger²,

Prenner³

2011

Advances in Biomimetics

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Section: Heavy Metals and Neurotoxic Cations: Multilamellar Vesiclesmentioning

confidence: 99%

Section: The Importance Of Lipid Model Systemsmentioning

confidence: 99%

Section: I M Y R I S T O Y L P H O S P H a T I D I C A C I D ( D M mentioning

confidence: 99%

Section: Heavy Metals and Neurotoxic Cations: Multilamellar Vesiclesmentioning

confidence: 99%

Section: Heavy Metals and Neurotoxic Cations: Multilamellar Vesiclesmentioning

confidence: 99%

See 3 more Smart Citations

Biomimetic Model Membrane Systems Serve as Increasingly Valuable in Vitro Tools

Le¹,

Litzenberger²,

Prenner³

2011

Advances in Biomimetics

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Cesium's Affects on Morphological Changes of Human Erythrocytes

Feng

2012

Chin. J. Chem.

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Cesium could play a toxic role in several pathological processes. Atomic force microscopy (AFM) was used to study morphological changes of human erythrocytes after incubating with different concentrations of CsCl, and the Raman spectra were used to study the effects of CsCl on the chemistry components of erythrocyte membrane. The AFM images showed that the "domain structures" that appeared after incubation with higher concentration of CsCl (150 mmol·L−1), are featured by the particles aggregated to form ranges and the separations among them enlarged to gorges, and this change may increase the permeability of cell membranes. The Raman results showed that the polar part of membrane phospholipid become more order and with the increasing of the concentration of CsCl, the longitudinal order of nonpolar parts first decreased and then increased. It is concluded that the aggregation of membrane proteins and the order changes of the phospholipid cause a change in the distribution and conformation of the phospholipid membrane. And the effects of CsCl on the erythrocyte membrane are mainly dependent on its concentration.

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Hg2+ and Cd2+ interact differently with biomimetic erythrocyte membranes

Gailer

Prenner

2008

Biometals

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In order to characterize the potentially deleterious effects of toxic Hg(2+) and Cd(2+) on lipid membranes, we have studied their binding to liposomes whose composition mimicked erythrocyte membranes. Fluorescence spectroscopy utilizing the concentration dependent quenching of Phen Green SK by Hg(2+) and Cd(2+) was found to be a sensitive tool to probe these interactions at metal concentrations < or =1 microM. We have systematically developed a metal binding affinity assay to screen for the interactions of Hg(2+) or Cd(2+) with certain lipid classes. A biomimetic liposome system was developed that contained four major lipid classes of erythrocyte membranes (zwitterionic lipids: phosphatidylcholine and phosphatidylethanolamine; negatively charged: phosphatidylserine and neutral: cholesterol). In contrast to Hg(2+), which preferentially bound to the negatively charged phosphatidylserine compared to the zwitterionic components, Cd(2+) bound stronger to the two zwitterionic lipids. Thus, the observed distinct differences in the binding affinity of Hg(2+) and Cd(2+) for certain lipid classes together with their known effects on membrane properties represent an important first step toward a better understanding the role of these interactions in the chronic toxicity of these metals.

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HgCl2 disrupts the structure of the human erythrocyte membrane and model phospholipid bilayers

Cited by 34 publications

References 28 publications

Biomimetic Model Membrane Systems Serve as Increasingly Valuable in Vitro Tools

Biomimetic Model Membrane Systems Serve as Increasingly Valuable in Vitro Tools

Cesium's Affects on Morphological Changes of Human Erythrocytes

Hg2+ and Cd2+ interact differently with biomimetic erythrocyte membranes

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