Hibernation-specific alternative splicing of the mRNA encoding cold-inducible RNA-binding protein in the hearts of hamsters

Sano, Yuichi; Shiina, Takahiko; Naitou, Kiyotada; Nakamori, Hiroyuki; Shimizu, Yasutake

doi:10.1016/j.bbrc.2015.04.135

Cited by 25 publications

(35 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar temperature-mediated changes in exon usage associated with RNA splicing and binding are also common in plants (Capovilla et al, 2015;Filichkin et al, 2015;Calixto et al, 2018). Even at the level of specific genes, variation in splicing patterns in killifish and zebrafish was observed for genes that have previously been highlighted for their responses and roles in cold acclimation, such as cold-inducible RNA binding protein (cirbp; Gracey et al, 2004;Sano et al, 2015). We also observed substantial evidence for enrichment of AS genes associated with contractile structures and functions, which is clearly consistent with changes in splicing associated with a central function of muscle tissue that is known to demonstrate plasticity in response to temperature change (Johnston et al, 1990;Johnston and Temple, 2002).…”

Section: Role Of Changes In Mrna Splicing Patterns In Plasticity As Amentioning

confidence: 86%

Patterns of alternative splicing in response to cold acclimation in fish

Healy

Schulte

2019

Journal of Experimental Biology

View full text Add to dashboard Cite

Phenotypic plasticity is an important aspect of an organism's response to environmental change that often requires the modulation of gene expression. These changes in gene expression can be quantitative, as a result of increases or decreases in the amounts of specific transcripts, or qualitative, as a result of the expression of alternative transcripts from the same gene (e.g. via alternative splicing of pre-mRNAs). Although the role of quantitative changes in gene expression in phenotypic plasticity is well known, relatively few studies have examined the role of qualitative changes. Here, we use skeletal muscle RNA-seq data from Atlantic killifish (Fundulus heteroclitus), threespine stickleback (Gasterosteus aculeatus) and zebrafish (Danio rerio) to investigate the extent of qualitative changes in gene expression in response to cold acclimation. Fewer genes demonstrated alternative splicing than differential expression as a result of cold acclimation; however, differences in splicing were detected for 426 to 866 genes depending on species, indicating that large numbers of qualitative changes in gene expression are associated with cold acclimation. Many of these alternatively spliced genes were also differentially expressed, and there was functional enrichment for involvement in muscle contraction among the genes demonstrating qualitative changes in response to cold acclimation. Additionally, there was a common group of 29 genes with cold-acclimation-mediated changes in splicing in all three species, suggesting that there may be a set of genes with expression patterns that respond qualitatively to prolonged exposure to cold temperatures across fishes.

show abstract

Section: Role Of Changes In Mrna Splicing Patterns In Plasticity As Amentioning

confidence: 86%

Patterns of alternative splicing in response to cold acclimation in fish

Healy

Schulte

2019

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…This transcript contains an additional insert with an internal stop codon at the open reading frame (ORF), leading to a truncated protein and aberrant function. In contrast, hibernating animals predominantly express the short isoform with a complete ORF . Overall, CIRP expression levels are altered in response to stress by versatile mechanisms, suggesting a wide range of adaptation to various external and internal challenges.…”

Section: Cirpmentioning

confidence: 99%

Extracellular CIRP (eCIRP) and inflammation

Aziz

Brenner

Wang

2019

Journal of Leukocyte Biology

158

188

View full text Add to dashboard Cite

Cold-inducible RNA-binding protein (CIRP) was discovered 2 decades ago while studying the mechanism of cold stress adaptation in mammals. Since then, the role of intracellular CIRP (iCIRP) as a stress-response protein has been extensively studied. Recently, extracellular CIRP (eCIRP) was discovered to also have an important role, acting as a damage-associated molecular pattern, raising critical implications for the pathobiology of inflammatory diseases. During hemorrhagic shock and sepsis, inflammation triggers the translocation of CIRP from the nucleus to the cytosol and its release to the extracellular space. eCIRP then induces inflammatory responses in macrophages, neutrophils, lymphocytes, and dendritic cells. eCIRP also induces endoplasmic reticulum stress and pyroptosis in endothelial cells by activating the NF-B and inflammasome pathways, and necroptosis in macrophages via mitochondrial DNA damage. eCIRP works through the TLR4-MD2 receptors. Studies with CIRP −/− mice reveal protection against inflammation, implicating eCIRP to be a novel drug target. Anti-CIRP Ab or CIRP-derived small peptide may have effective therapeutic potentials in sepsis, acute lung injury, and organ ischemia/reperfusion injuries. The current review focuses on the pathobiology of eCIRP by emphasizing on signal transduction machineries, leading to discovering novel therapeutic interventions targeting eCIRP in various inflammatory diseases. K E Y W O R D S ALI, CIRP, DAMP, eCIRP, hemorrhage, inflammation, ischemia/reperfusion, macrophage, neutrophils, sepsis PMN, polymorphonuclear neutrophils; RA, rheumatoid arthritis; RM, reverse migrated; rTEM, reverse transendothelial migration; STAT3, signal transducer and activator of transcription 3; TRPV, transient receptor potential vanilloid; UC, ulcerative colitis telomerase maintenance, 7 stress adaptation, 8 and tumor formation and progression. 9,10 These iCIRP activities, which allow cells to cope with various cellular stress conditions, have been reviewed in detail. 8 In contrast to iCIRP, extracellular CIRP (eCIRP) was recently discovered to act as a damage-associated molecular pattern (DAMP) promoting inflammation and injury. 11 The identification of eCIRP as a new DAMP has originated a new era of investigation in inflammation pathobiology. Serum levels of eCIRP not only were found to be elevated in individuals admitted to the intensive care unit (ICU)with hemorrhagic shock (HS), but also correlated with organ injury. 11 Furthermore, it was demonstrated that eCIRP was able to promote inflammation independently. 11 In cells subjected to hypoxia, CIRP translocates from its usual location in the nucleus to the cytosol 1,12 and is then released to the extracellular space. 11 eCIRP then acts as a DAMP increasing macrophage production of proinflammatory cytokines. 11 Since its original discovery as a DAMP, eCIRP's emerging proinflammatory role has been progressively extended to include additional target cells, such as neutrophils, lymphocytes, epithelial cells, and endothelial cells. 13-2...

show abstract

“…The cold-inducible expression of Cirbp mRNA has previously been attributed to various mechanisms, ranging from SP1-mediated transcriptional control 11 to alternative splicing 12 and alternative transcription start-site usage. 13 In an attempt to resolve the issue, we used several quantitative and qualitative techniques to study Cirbp expression in NIH 3T3 cells and mouse livers.…”

Section: Tracing the Cirbp Tracksmentioning

confidence: 99%

Posttranscriptional mechanisms controlling diurnal gene expression cycles by body temperature rhythms

Gotić

Schibler

2017

RNA Biology

View full text Add to dashboard Cite

In mammals, body temperature oscillates in a daily fashion around a set point of 36°C-37°C. These fluctuations are controlled by the circadian master clock residing in the brain's suprachiasmatic nucleus and, despite their small amplitudes, contribute to the diurnal expression of genes throughout the organism. By focusing on the mechanisms underlying the temperature-dependent accumulation of the cold-inducible RNA-binding protein CIRBP - a factor involved in the tuning of amplitude and phase in circadian clocks of peripheral tissues - we have recently identified a novel mechanism governing temperature-dependent gene expression. This mechanism involves the differential spicing efficiency of primary RNA transcripts under different temperature conditions and thereby determines the fraction of Cirbp pre-mRNA processed into mature mRNA. A genome-wide transcriptome analysis revealed that this mechanism affects the output of hundreds of genes. Here we discuss our findings and future directions toward the identification of specific factors and parameters governing temperature-sensitive splicing efficacy.

show abstract

Hibernation-specific alternative splicing of the mRNA encoding cold-inducible RNA-binding protein in the hearts of hamsters

Cited by 25 publications

References 16 publications

Patterns of alternative splicing in response to cold acclimation in fish

Patterns of alternative splicing in response to cold acclimation in fish

Extracellular CIRP (eCIRP) and inflammation

Posttranscriptional mechanisms controlling diurnal gene expression cycles by body temperature rhythms

Contact Info

Product

Resources

About