Ultralight scalars, if they exist as theorized, could form clouds around rapidly rotating black holes. Such clouds are expected to emit continuous, quasimonochromatic gravitational waves that could be detected by LIGO and Virgo. Here we present results of a directed search for such signals from the Cygnus X-1 binary, using data from Advanced LIGO's second observing run. We find no evidence of gravitational waves in the 250-750 Hz band. Without incorporating existing measurements of the Cygnus X-1 black hole spin, our results disfavor boson masses in 5.8 ≤ µ/(10 −13 eV) ≤ 8.6, assuming that the black hole was born 5 × 10 6 years ago with a nearly-extremal spin. We then focus on a string axiverse scenario, in which self-interactions enable a cloud for high black-hole spins consistent with measurements for Cygnus X-1. In that model, we constrain the boson masses in 9.6 ≤ µ/(10 −13 eV) ≤ 15.5 for a decay constant fa ∼ 10 15 GeV. Future application of our methods to other sources will yield improved constraints.Introduction.-Ultralight scalar (spin 0) or vector (spin 1) boson particles have been theorized under several frameworks to solve problems in particle physics, high-energy theory and cosmology [1][2][3][4][5][6][7][8]. If such a new fundamental field exists, its occupancy number should superradiantly grow around fast-spinning black holes (BHs). This occurs when ω µ /m < Ω BH , where ω µ = µ/ is the characteristic angular frequency of a boson with rest energy µ, m is the boson azimuthal quantum number with respect to the BH's rotation axis, and Ω BH is the angular speed of the outer horizon. The superradiant instability is maximized when the Compton wavelength of the particle is comparable to the characteristic length of the BH, meaning hc/µ ∼ GM/c 2 . If these conditions are satisfied, the number of ultralight bosons around the BH grows exponentially, forming a macroscopic cloud holding up to ∼10% of the BH mass. This cloud can have a long lifetime, during which it generates continuous, quasi-monochromatic gravitational waves (GWs) [9][10][11][12][13][14][15][16].