Hierarchical Assembly of Viral Nanotemplates with Encoded Microparticles via Nucleic Acid Hybridization

Tan, Wen See; Lewis, Christina L.; Horelik, Nicholas; Pregibon, Daniel C.; Doyle, Patrick S.; Yi, Hyunmin

doi:10.1021/la802089q

Cited by 41 publications

(54 citation statements)

References 51 publications

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“…TMV1cys was fluorescently labeled as described previously 30. Briefly, purified TMV1cys was incubated at room temperature for 2 h with 10-fold molar excess of fluorescein-5-maleimide (Biotium, Hayward, CA) in 100mM Tris Buffer, pH 7.0.…”

Section: Methodsmentioning

confidence: 99%

Microfluidic Fabrication of Hydrogel Microparticles Containing Functionalized Viral Nanotemplates

Lewis¹,

Lin²,

Yang³

et al. 2010

Langmuir

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We demonstrate rapid microfluidic fabrication of hybrid microparticles composed of functionalized viral nanotemplates directly embedded in polymeric hydrogels. Specifically, genetically modified tobacco mosaic virus (TMV) templates were covalently labeled with fluorescent markers or metalized with palladium (Pd) nanoparticles (Pd-TMV), then suspended in a poly(ethylene glycol)-based solution. Upon formation in a flow-focusing device, droplets were photopolymerized with UV light to form microparticles. Fluorescence and confocal microscopy images of microparticles containing fluorescently labeled TMV show uniform distribution of TMV nanotemplates throughout the microparticles. Catalytic activity, via the dichromate reduction reaction, is also demonstrated with microparticles containing Pd-TMV complexes. Additionally, Janus microparticles were fabricated containing viruses embedded in one side and magnetic nanoparticles in the other, that enabled simple separation from bulk solution. These results represent a facile route to directly harness the advantages of viral nanotemplates into a readily usable and stable 3D assembled format.

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Section: Methodsmentioning

confidence: 99%

Microfluidic Fabrication of Hydrogel Microparticles Containing Functionalized Viral Nanotemplates

Lewis¹,

Lin²,

Yang³

et al. 2010

Langmuir

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“…[70] Virus, such as Tobacco Mosaic Virus (TMV), have also been readily incorporated into bar-coded particles formed using SFL. [71] …”

Section: Composite Particlesmentioning

confidence: 99%

The Synthesis and Assembly of Polymeric Microparticles Using Microfluidics

2009

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The controlled synthesis of micrometer‐sized polymeric particles bearing features such as nonspherical shapes and spatially segregated chemical properties is becoming increasingly important. Such particles can enable fundamental studies on self‐assembly and suspension rheology, as well as be used in applications ranging from medical diagnostics to photonic devices. Microfluidics has recently emerged as a very promising route to the synthesis of such polymeric particles, providing fine control over particle shape, size, chemical anisotropy, porosity, and core/shell structure. This progress report summarizes microfluidic approaches to particle synthesis using both droplet‐ and flow‐lithography‐based methods, as well as particle assembly in microfluidic devices. The particles formed are classified according to their morphology, chemical anisotropy, and internal structure, and relevant examples are provided to illustrate each of these approaches. Emerging applications of the complex particles formed using these techniques and the outlook for such processes are discussed.

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“…5D) [78], and multilayered microparticles [79]. This photolithographic microfluidic technique also made it possible to produce encoded microparticles [80], consisting of spatially discrete regions with information, an internal control, and capturing DNAs. Along the same lines, microparticles with core/shell or multiple core/shell structure were prepared by the Weitz group.…”

Section: Anisotropic Compartmentalizationmentioning

confidence: 99%