Hierarchical Flower-like WO₃ Nanospheres Decorated with Bimetallic Au and Pd for Highly Sensitive and Selective Detection of 3-Hydroxy-2-butanone Biomarker

Abstract

Listeria monocytogenes, which is abundant in environment, can lead to many kinds of serious illnesses and even death. Nowadays, indirectly detecting the metabolite biomarker of L. monocytogenes, 3-hydroxy-2-butanone, has been verified to be an effective way to evaluate the contamination of L. monocytogenes. However, this detection approach is still limited by sensitivity, selectivity, and ppb-level detection limit. Herein, low-cost and highly sensitive and selective 3-hydroxy-2-butanone sensors have been proposed based on the bimetallic AuPd decorated hierarchical flower-like WO3 nanospheres. Notably, the 1.0 wt % AuPd-WO3 based sensors displayed the highest sensitivity (R a/R g = 84 @ 1 ppm) at 250 °C. In addition, the sensors showed outstanding selectivity, rapid response/recovery (8/4 s @ 10 ppm), and low detection limit (100 ppb). Furthermore, the evaluation of L. monocytogenes with high sensitivity and specificity has been achieved using 1.0 wt % AuPd-WO3 based sensors. Such a marvelous sensing performance benefits from the synergistic effect of bimetallic AuPd nanoparticles, which lead to thicker electron depletion layer and increased adsorbed oxygen species. Meanwhile, the unique hierarchical nanostructure of the flower-like WO3 nanospheres benefits the gas-sensing performance. The AuPd-WO3 nanosphere-based sensors exhibit a particular and highly selective method to detect 3-hydroxy-2-butanone, foreseeing a feasible route for the rapid and nondestructive evaluation of foodborne pathogens.

WO3 Nanoplates Decorated with Au and SnO2 Nanoparticles for Real-Time Detection of Foodborne Pathogens

“…The homemade portable sensor displayed a rapid sensing response toward 25 ppm 3H-2B at 140 °C, which was comparable with the sensing information obtained from the gas-response instrument. The feasibility of the on-site monitoring of Listeria monocytogenes by detecting the concentration of 3H-2B has been demonstrated [ 13 , 46 ]; thus, these results suggest that a portable gas sensor holds great potential for the rapid identification of foodborne pathogens.…”

“…For example, Kaur et al reported a selective sensing behavior toward both oxidizing and reducing gas analytes by employing NiO/NiWO 4 /WO 3 heterojunctions [ 10 ]. In addition, decorating MOSs with noble metals (Ag, Au, Pd, and Pt) has been proposed as an effective method to promote the sensing performance at lower operating temperatures [ 13 , 14 , 15 , 16 ]. For instance, WO 3 nanoflowers decorated with PtAu bimetallic nanocrystals led to a high response to 20 ppm n-pentanol at 200 °C, a response that was 105 times higher than that for pure WO 3 [ 14 ].…”

“…Listeria monocytogenes is a highly contagious foodborne pathogen that causes listeriosis outbreaks primarily in immunocompromised individuals [ 13 ]. It can be found in various food sources such as vegetables, meat, seafood, and dairy products and has the ability to survive and even grow under refrigeration and other food preservation measures [ 20 ].…”

Oxygen Vacancy Engineering of FeOx toward Oxygen-Tolerant Hydrogen Peroxide Reduction for Reliable Bioassays

“…Heterogeneous catalysis plays an important role in energy conversion, , biomass transformation, , and the chemical industry. , The development of heterogeneous catalysts with high selectivity has attracted the attention of many researchers because it meets the requirements of green and sustainable chemistry . Diverse tactics, including composition regulation, , structure design, , surface modification, , and support selection, , have been developed to improve selectivity. Among these approaches, defect regulation for the catalyst stands out .…”

Multiplex nanozymatic biosensing of Salmonella on a finger-actuated microfluidic chip

“…Pathogenic bacteria are responsible for many foodborne diseases, and Salmonella typhimurium is a representative one, which is often found in pork and chicken samples. 1 Bacterial contamination may happen in all the links of food supply chains, such as processing, transportation and sale. 2 Therefore, bacterial screening is important but is faced with big challenges.…”