The article presents validation argumentation of the novel method of evaluating resistance to surface wetting with different liquids of protective materials intended for polymer protective gloves based on the three parameters: water permeability index, non-wettability index and absorption index. Using our own method of evaluating resistance to surface wetting, it was shown that the knurled structure of the palm part of polymer protective gloves may inhibit transport of harmful and hazardous liquids outside the area of the protective glove. Currently, there is lack of objectifying methods for evaluation of surface wettability focused on the mentioned aspects. In view of the above facts, an original method for evaluating the resistance of protective materials to surface wetting with mineral oils and water has been invented and validated. It was assumed that the non-wettability index will be subjected to metrological analysis. Consequently, the validation process refers to this index. A precise assessment of the uncertainty budget of the individual components was obtained. On the basis of the obtained results, the measurement errors that may affect the quality and reliability of the test result performed in the laboratory were identified.