Hierarchical micro-nano structured Ti6Al4V surface topography via two-step etching process for enhanced hydrophilicity and osteoblastic responses

Moon, Byeong‐Seok; Kim, Sung-Won; Kim, Hyoun‐Ee; Jang, Tae‐Sik

doi:10.1016/j.msec.2016.12.064

Cited by 63 publications

(37 citation statements)

References 53 publications

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“…The surface morphology of implants is another crucial point affecting the adhesion and differentiation of osteoblasts during the initial phase of osseointegration and long-term bone remodelling [19,20]. The micro-nano dual structure exhibited rough surface characteristic and favourable wettability, which enhanced hydrophilicity and cell adhesion [21,22]. Therefore, the biological properties of the MAO coatings were further analysed.…”

Section: Discussionmentioning

confidence: 99%

Micro/nano-hierarchical structured TiO ₂ coating on titanium by micro-arc oxidation enhances osteoblast adhesion and differentiation

Pan

Abdullah

et al. 2019

R. Soc. open sci.

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Nano-structured and micro/nano-hierarchical structured TiO 2 coatings were produced on polished titanium by the micro-arc oxidation (MAO) technique. This study was conducted to screen a suitable structured TiO 2 coating for osteoblast adhesion and differentiation in dental implants. The formulation was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and wettability testing. Adhesion, proliferation and osteogenic differentiation of MG63 cells were analysed by SEM, Cell Counting Kit-8 (CCK-8) and quantitative real-time PCR. The micro/nano-hierarchical structured TiO 2 coating with both slots and pores showed the best morphology and wettability. XRD analysis revealed that rutile predominated along with a minor amount of anatase in both TiO 2 coatings. Adhesion and extension of MG63 cells on the micro/nano-hierarchical structured TiO 2 coating were the most favourable. MG63 cells showed higher growth rates on the micro/nano-hierarchical structured TiO 2 coating at 1 and 3 days. Osteogenic-related gene expression was markedly increased in the micro/nano-hierarchical structured TiO 2 coating group compared with the polished titanium group at 7, 14 and 21 days. These results revealed the micro/nano-hierarchical structured TiO 2 coating as a promising surface modification and suitable biomaterial for use with dental implants.

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Section: Discussionmentioning

confidence: 99%

Micro/nano-hierarchical structured TiO ₂ coating on titanium by micro-arc oxidation enhances osteoblast adhesion and differentiation

Pan

Abdullah

et al. 2019

R. Soc. open sci.

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“…However, the osteogenic capacity of bone cells on this macro/mesoporous structure was not investigated. In several reports, hierarchical structures have been shown to exhibit synergistic effects on the enhancement of osteogenic activity when compared with solo topography . Therefore, we assume that modification of the Ti‐based alloy surface with macro/mesoporous structures would be beneficial for osseointegration.…”

Section: Introductionmentioning

confidence: 98%

Effects of the hierarchical macro/mesoporous structure on the osteoblast‐like cell response

Zhang

Elkhooly

Huang

et al. 2018

J Biomedical Materials Res

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To improve the success of medical devices, implants with strong surface bioactivity are urgently required. Coatings with a macroporous structure produced by micro-arc oxidation possess advantages, such as strong adhesion to substrate and excellent resistance to wear and corrosion. Mesoporous structures contain pores with sizes of 2-50 nm, which can endow the biomaterials with the ability to enhance osteogenesis and to be loaded with diverse drugs. Thus, in this study, we aimed to evaluate the effects of both macroporous and mesoporous structures using a hierarchical macro/mesoporous structure to modify the titanium implant surface. The behaviors of SaOS-2 human osteosarcoma cells on the macro/mesoporous structure, including initial adhesion, proliferation, alkaline phosphatase (ALP) activity, and collagen secretion, were investigated. Cells that attached on the macro/mesoporous surface showed the highest cell numbers and greatest spreading area after incubation for 1, 2, and 4 h compared with the polished smooth substrate and macroporous surface in the presence of fetal bovine serum (FBS). However, in the absence of FBS, cell adhesion on the polished substrate, macroporous structure, and macro/mesoporous structure did not differ significantly. Cell proliferation on the macroporous and macro/mesoporous surfaces increased compared with that on the smooth substrate surface. Furthermore, ALP activity and collagen secretion were enhanced on the macro/mesoporous structure. Our findings provided important insights into the cellular responses to macro/mesoporous structures in the field of implant surface modification. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1896-1902, 2018.

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“…Nanostructure surface can directly interact with some proteins and cell membrane receptors, effectively enhancing cell functions [8]. Micro/nano hierarchical topography on implant materials bear a closer resemblance to the features of the natural extracellular matrix, and may be biologically friendly in multiple cell behaviors [9]. Recently, numerous studies regarding the positive osteogenic effects of hierarchical micro/nanostructure surfaces have been reported [10].…”

Section: Introductionmentioning

confidence: 99%

Micro/nanostructured TiO ₂ /ZnO coating enhances osteogenic activity of SaOS-2 cells

Zhang

Liu

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Although titanium implants account for a large proportion of the commercial dental market, their bioactivity are inadequate in many applications. A micro-and nano-scale hierarchical surface topography of the implant is suggested for rapid osseointegration from the biomimetic perspective. Moreover, Zinc (Zn) is an essential element in the skeletal system. Thus, a micro/nanostructured TiO 2 /ZnO coating, produced by micro-arc oxidation, and hydrothermal treatment, and heat treatment, was designed to endow the implant surface with enhanced osteogenic capacity. Physiochemical properties and biological effects of this coating were investigated in our study. The annealed micro/nanostructured TiO 2 / ZnO coating exhibited higher hydrophilicity and fibronectin adsorption ability compared to the microarc oxidation modified TiO 2 coating. SaOS-2 cells grown on the annealed micro/nanostructured TiO 2 / ZnO coating showed increased alkaline phosphatase activity and collagen secretion, and immunofluorescence labeling revealed an upregulation of osteopontin, collagen type i and osteocalcin. The micro/ nanostructure and incorporation of Zn were considered to perform positive effect on the enhanced osteogenic activity of SaOS-2 cells. In conclusion, the micro/nanostructured TiO 2 /ZnO structure is simple, stable, and easy to produce and scale up, has promising applications in the surface modification of titanium implants.

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Hierarchical micro-nano structured Ti6Al4V surface topography via two-step etching process for enhanced hydrophilicity and osteoblastic responses

Cited by 63 publications

References 53 publications

Micro/nano-hierarchical structured TiO ₂ coating on titanium by micro-arc oxidation enhances osteoblast adhesion and differentiation

Micro/nano-hierarchical structured TiO ₂ coating on titanium by micro-arc oxidation enhances osteoblast adhesion and differentiation

Effects of the hierarchical macro/mesoporous structure on the osteoblast‐like cell response

Micro/nanostructured TiO ₂ /ZnO coating enhances osteogenic activity of SaOS-2 cells

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Hierarchical micro-nano structured Ti6Al4V surface topography via two-step etching process for enhanced hydrophilicity and osteoblastic responses

Cited by 63 publications

References 53 publications

Micro/nano-hierarchical structured TiO 2 coating on titanium by micro-arc oxidation enhances osteoblast adhesion and differentiation

Micro/nano-hierarchical structured TiO 2 coating on titanium by micro-arc oxidation enhances osteoblast adhesion and differentiation

Effects of the hierarchical macro/mesoporous structure on the osteoblast‐like cell response

Micro/nanostructured TiO 2 /ZnO coating enhances osteogenic activity of SaOS-2 cells

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Product

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Micro/nano-hierarchical structured TiO ₂ coating on titanium by micro-arc oxidation enhances osteoblast adhesion and differentiation

Micro/nano-hierarchical structured TiO ₂ coating on titanium by micro-arc oxidation enhances osteoblast adhesion and differentiation

Micro/nanostructured TiO ₂ /ZnO coating enhances osteogenic activity of SaOS-2 cells