Hierarchical-Multiplex DNA Patterns Mediated by Polymer Brush Nanocone Arrays That Possess Potential Application for Specific DNA Sensing

Abstract

This paper provides a facile and cost-efficient method to prepare single-strand DNA (ssDNA) nanocone arrays and hierarchical DNA patterns that were mediated by poly(2-hydroxyethyl methacrylate) (PHEMA) brush. The PHEMA brush nanocone arrays with different morphology and period were fabricated via colloidal lithography. The hierarchical structure was prepared through the combination of colloidal lithography and traditional photolithography. The DNA patterns were easily achieved via grafting the amino group modified ssDNA onto the side chain of polymer brush, and the anchored DNA maintained their reactivity. The as-prepared ssDNA nanocone arrays can be applied for target DNA sensing with the detection limit reaching 1.65 nM. Besides, with the help of introducing microfluidic ideology, the hierarchical-multiplex DNA patterns on the same substrate could be easily achieved with each kind of pattern possessing one kind of ssDNA, which are promising surfaces for the preparation of rapid, visible, and multiplex DNA sensors.

Ordered Hybrid Micro/Nanostructures and Their Optical Applications

“…(IV) Fluorescence image of the hierarchical‐multiplex DNA patterns after each section was bonded with specific FITC‐conjugate complementary oligonucleotides. Reproduced with permission . Copyright 2015, American Chemical Society.…”

“…The fluorescence detection for target DNA with the detection limit of 1.65 × 10 −9 m was obtained, which was dependent on the abundant reaction sites of polymer brush. Moreover, multiplex DNA sensors maintained specificity by introducing the microfluidic ideology, which made the surfaces promising for the rapid, visible, and clinical diagnostic …”

Development of Sphere-Polymer Brush Hierarchical Nanostructure Substrates for Fabricating Microarrays with High Performance

“…These studies efficiently improve the molecular loading capability of substrates through increasing the surface areas of substrate materials. Polymer brushes containing carbohydrate residues (or DNA) exhibit good binding specificity because polymer brushes, as flexible polymer chains, can efficiently decrease steric hindrance between the substrate surface and target molecules. − A ZnO nanorod-polymer brush hierarchically nanostructured substrate has been employed as a platform for highly sensitive detection of cancer biomarkers in human serum for the first time . Through combining a 3D surface and densely packed polymer brushes, the substrate can further increase the loading amount of biomolecules and provide good accessibility to analytes.…”

Poly(glycidyl methacrylate-co-2-hydroxyethyl methacrylate) Brushes as Peptide/Protein Microarray Substrate for Improving Protein Binding and Functionality

“…Among these coatings, polymer brushes are attractive 3D substrates because the polymer brushes have several advantages including facile in situ synthesis, high biomolecule immobilization capacity, and accessible scaffolds with sufficient space for biomolecules binding. − For instance, aldehyde-functionalized poly­(2-hydroxyethyl methacrylate) (PHEMA) brushes were employed as platforms for the immobilization and hybridization of oligonucleotides . Then PHEMA brush nanocone arrays with various morphologies and periods were prepared as substrates for DNA detection, which are rapid, visible, and multiplex . However, both the works require subsequent functionalization after polymerization.…”

“…19 Then PHEMA brush nanocone arrays with various morphologies and periods were prepared as substrates for DNA detection, which are rapid, visible, and multiplex. 20 However, both the works require subsequent functionalization after polymerization. Hu et al proposed an antigen microarraybased competitive mycotoxins immunoassay on a POEGMAco-GMA brush substrate.…”