Hierarchical porous ECM scaffolds incorporating GDF-5 fabricated by cryogenic 3D printing to promote articular cartilage regeneration

Wu, Jiarui; Fu, Liwei; Yan, Zineng; Yu, Yang; Yin, Han; Li, Pinxue; Yuan, Xun; Ding, Zhengang; Kang, Teng; Tian, Zhigang; Liao, Zhiyao; Tian, Guangzhao; Ning, Chao; Li, Yuguo; Xiang, Sheng; Chen, Mingxue; Liu, Shuyun; Guo, Quanyi

doi:10.1186/s40824-023-00349-y

Cited by 19 publications

(9 citation statements)

References 36 publications

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“…The scaffold degradation rate should balance with ECM deposition induced by stem cells. Too rapid degradation could lead to larger porosity, reducing ECM deposition, while a controlled degradation rate facilitates cell proliferation and ECM formation, enhancing cell permeation and intercellular interactions [ 32 ]. These results indicated that SD-MSs and RSD-MSs have suitable structural stability and degradation rates, likely benefiting ECM deposition and providing favorable conditions for constructing cartilage organoids.…”

Section: Resultsmentioning

confidence: 99%

Boosting cartilage repair with silk fibroin-DNA hydrogel-based cartilage organoid precursor

Shen,

Wang,

et al. 2024

Bioactive Materials

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Section: Resultsmentioning

confidence: 99%

Boosting cartilage repair with silk fibroin-DNA hydrogel-based cartilage organoid precursor

Shen,

Wang,

et al. 2024

Bioactive Materials

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“…Numerous studies have shown that compared with other biomaterials, decellularized extracellular matrices (ECMs) can provide a good microenvironment for cell growth and are more beneficial for the repair of joint defects. Low-temperature-deposited 3D-printed decellularized chondrocyte ECM scaffolds have shown good biocompatibility and porosity in early research by our project team, and these scaffolds provide a good microenvironment for bone marrow MSCs (BMSCs) [18,19].…”

Section: Graphical Abstractmentioning

confidence: 99%

“…Total RNA was extracted from the BMSC pellets after 14 days of culture using a Cell Total RNA Isolation Kit. The detailed PCR experimental procedures that were used were described in a previous study [19].…”

Section: Effect Of Different Apoevs On Bmsc Chondrogenic Differentiationmentioning

confidence: 99%

Apoptotic extracellular vesicles derived from hypoxia-preconditioned mesenchymal stem cells within a modified gelatine hydrogel promote osteochondral regeneration by enhancing stem cell activity and regulating immunity

Ding,

Yan,

Yuan

et al. 2024

J Nanobiotechnol

Self Cite

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Due to its unique structure, articular cartilage has limited abilities to undergo self-repair after injury. Additionally, the repair of articular cartilage after injury has always been a difficult problem in the field of sports medicine. Previous studies have shown that the therapeutic use of mesenchymal stem cells (MSCs) and their extracellular vesicles (EVs) has great potential for promoting cartilage repair. Recent studies have demonstrated that most transplanted stem cells undergo apoptosis in vivo, and the apoptotic EVs (ApoEVs) that are subsequently generated play crucial roles in tissue repair. Additionally, MSCs are known to exist under low-oxygen conditions in the physiological environment, and these hypoxic conditions can alter the functional and secretory properties of MSCs as well as their secretomes. This study aimed to investigate whether ApoEVs that are isolated from adipose-derived MSCs cultured under hypoxic conditions (hypoxic apoptotic EVs [H-ApoEVs]) exert greater effects on cartilage repair than those that are isolated from cells cultured under normoxic conditions. Through in vitro cell proliferation and migration experiments, we demonstrated that H-ApoEVs exerted enhanced effects on stem cell proliferation, stem cell migration, and bone marrow derived macrophages (BMDMs) M2 polarization compared to ApoEVs. Furthermore, we utilized a modified gelatine matrix/3D-printed extracellular matrix (ECM) scaffold complex as a carrier to deliver H-ApoEVs into the joint cavity, thus establishing a cartilage regeneration system. The 3D-printed ECM scaffold provided mechanical support and created a microenvironment that was conducive to cartilage regeneration, and the H-ApoEVs further enhanced the regenerative capacity of endogenous stem cells and the immunomodulatory microenvironment of the joint cavity; thus, this approach significantly promoted cartilage repair. In conclusion, this study confirmed that a ApoEVs delivery system based on a modified gelatine matrix/3D-printed ECM scaffold together with hypoxic preconditioning enhances the functionality of stem cell-derived ApoEVs and represents a promising approach for promoting cartilage regeneration. Graphical Abstract

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“…It maintains the 3D architecture of the original tissues and it may retain several cell growth factors, which can enhance the growth, migration, proliferation, or differentiation of seeded cells [56]. It has been used to synthesize hydrogels for 3D printing of cartilage [57][58][59][60], ovarian [61], testicular [62], pancreatic [63,64], and skin tissues [65][66][67].…”

Section: Nature-derived Polymersmentioning

confidence: 99%

Current Biomedical Applications of 3D-Printed Hydrogels

Barcena,

Dhal,

Patel

et al. 2023

Gels

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Three-dimensional (3D) printing, also known as additive manufacturing, has revolutionized the production of physical 3D objects by transforming computer-aided design models into layered structures, eliminating the need for traditional molding or machining techniques. In recent years, hydrogels have emerged as an ideal 3D printing feedstock material for the fabrication of hydrated constructs that replicate the extracellular matrix found in endogenous tissues. Hydrogels have seen significant advancements since their first use as contact lenses in the biomedical field. These advancements have led to the development of complex 3D-printed structures that include a wide variety of organic and inorganic materials, cells, and bioactive substances. The most commonly used 3D printing techniques to fabricate hydrogel scaffolds are material extrusion, material jetting, and vat photopolymerization, but novel methods that can enhance the resolution and structural complexity of printed constructs have also emerged. The biomedical applications of hydrogels can be broadly classified into four categories—tissue engineering and regenerative medicine, 3D cell culture and disease modeling, drug screening and toxicity testing, and novel devices and drug delivery systems. Despite the recent advancements in their biomedical applications, a number of challenges still need to be addressed to maximize the use of hydrogels for 3D printing. These challenges include improving resolution and structural complexity, optimizing cell viability and function, improving cost efficiency and accessibility, and addressing ethical and regulatory concerns for clinical translation.

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Hierarchical porous ECM scaffolds incorporating GDF-5 fabricated by cryogenic 3D printing to promote articular cartilage regeneration

Cited by 19 publications

References 36 publications

Boosting cartilage repair with silk fibroin-DNA hydrogel-based cartilage organoid precursor

Boosting cartilage repair with silk fibroin-DNA hydrogel-based cartilage organoid precursor

Apoptotic extracellular vesicles derived from hypoxia-preconditioned mesenchymal stem cells within a modified gelatine hydrogel promote osteochondral regeneration by enhancing stem cell activity and regulating immunity

Current Biomedical Applications of 3D-Printed Hydrogels

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