Hierarchical scaffolds via combined macro- and micro-phase separation

George, Peter A.; Quinn, K.; Cooper-White, Justin J.

doi:10.1016/j.biomaterials.2009.09.094

Cited by 55 publications

(39 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10,18 By transitioning the polymer-DMC droplet from 25 C to 3 C upon exit from the glass microfluidic device, within a short time frame (less than 2 minutes) we were thus able to induce a spinodal phase separation event via TIPS, as exemplified by the characteristic spinodal (bicontinuous) architecture 19 of the internals of the microspheres (Fig. 2c).…”

Section: Interplay Of Diffusion-induced Phase Separation (Dips) and Tmentioning

confidence: 96%

“…10 In this paper, we significantly extend upon this work by employing a capillary microfluidic device to produce BCP microspheres formed by two distinct phase separation pathways, diffusion induced phase separation (DIPS) and TIPS. The resulting microspheres display topographical and morphological characteristics that can be attributed to both of these phase separation events, leading to a useful and defined end product that may be used as a completely defined surface substrate in stem cell research 11,12 and in future regenerative medicine strategies.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication of nanopatterned, porous microspheres using a glass capillary microfluidic device

et al. 2012

Self Cite

View full text Add to dashboard Cite

This paper describes the use of a microfluidic device to produce porous, hierarchical nano-patterned microspheres. Within this device, explicit control over the micro-phase separation and macro-phase solidification behaviour of a polystyrene-polyethylene oxide di-block copolymer dissolved in an organic (polar) solvent droplet has been achieved through the tailoring of the staged onset of diffusion induced phase separation (DIPS) and thermally induced phase separation (TIPS) events post dispersion in an aqueous continuous phase. Due to water and the organic solvent (dimethylene carbonate (DMC)) being partially miscible, the organic solvent within the dispersed phase diffuses very slowly out of the polymer-loaded droplets into the continuous transport fluid (water) as they flow down the microchannel. This permits controlled shrinkage of the droplets (and hence sizing), and the eventual condensation of the external surface of the polymer microbeads, prior to the timed onset of TIPS, which promotes the formation of a highly porous internal structure (via liquid-liquid macrophase separation) within the microbeads. The thickness of the external layer can be controlled by varying the timeframe for DIPS, along with porosity of the internals of these hierarchical microbeads by varying the TIPS parameters. The resulting monodisperse hierarchical microspheres are comprised of external surfaces decorated with nano-scale self-assembled block copolymer domains, while the internal structure is highly porous and all surfaces display the same nano-scale domains. The hydrophilic nature of the PEO nano-domains results in these microparticles being near neutrally buoyant in water and bioactive moieties can be presented on the terminal ends of the PEO chains in each domain. These novel microparticles are thus seen as ideal for bead-based cell culture applications and the use of this methodology with degradable block copolymers ideal for future application in regenerative medicine.

show abstract

Section: Interplay Of Diffusion-induced Phase Separation (Dips) and Tmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Fabrication of nanopatterned, porous microspheres using a glass capillary microfluidic device

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…Further, by changing the concentration of the block copolymer, the spacing and patterning of the peptide sequences could be controlled. In another study, George et al extended this concept by forming porous three-dimensional scaffolds from block copolymers [56], thus creating nanostructured block copolymer in a configuration that is useful for tissue engineering. This highlights another advantage of block copolymer generated morphology, ease of processing.…”

Section: Block Copolymers and Protein Patterningmentioning

confidence: 98%

“…Self-assembled methodologies such as peptide amphiphiles [46,47], dendrimers [48], and liquid crystals [49] are also utilized to generate regular nanostructures. Despite their well-documented ability to generate nanostructured patterns, there are relatively few examples of block copolymers generating bioactive surfaces [50][51][52][53][54][55][56]. Block copolymers have typically been used to generate micelles for drug delivery [57][58][59] or as a method to control biodegradation or surface energy [60].…”

Section: Nanostructured Biomaterialsmentioning

confidence: 99%

Modulating Protein Adhesion and Conformation with Block Copolymer Surfaces

Schricker

Palacio

Bhushan

2014

Handbook of Nanomaterials Properties

View full text Add to dashboard Cite

“…Topdown approaches involve creating patterns through some type of lithography or etching [37], such as soft lithography [38][39][40] or electron beam etching [41,42]. Bottom-up approaches involve self-assembly processes such as liquid crystals [43], supramolecular chemistry [44][45][46], self-assembled monolayers [14,[47][48][49] or block copolymers [25,37,[50][51][52][53][54][55][56][57][58][59][60][61][62][63]. In addition, these systems can be used to understand and model the material-cell or material-protein interactions.…”

Section: (B) Engineering Surfaces To Control Protein Adsorptionmentioning

confidence: 99%

Designing nanostructured block copolymer surfaces to control protein adhesion

Schricker

Palacio

Bhushan

2012

Phil. Trans. R. Soc. A.

View full text Add to dashboard Cite

The profile and conformation of proteins that are adsorbed onto a polymeric biomaterial surface have a profound effect on its in vivo performance. Cells and tissue recognize the protein layer rather than directly interact with the surface. The chemistry and morphology of a polymer surface will govern the protein behaviour. So, by controlling the polymer surface, the biocompatibility can be regulated. Nanoscale surface features are known to affect the protein behaviour, and in this overview the nanostructure of self-assembled block copolymers will be harnessed to control protein behaviour. The nanostructure of a block copolymer can be controlled by manipulating the chemistry and arrangement of the blocks. Random, A-B and A-B-A block copolymers composed of methyl methacrylate copolymerized with either acrylic acid or 2-hydroxyethyl methacrylate will be explored. Using atomic force microscopy (AFM), the surface morphology of these block copolymers will be characterized. Further, AFM tips functionalized with proteins will measure the adhesion of that particular protein to polymer surfaces. In this manner, the influence of block copolymer morphology on protein adhesion can be measured. AFM tips functionalized with antibodies to fibronectin will determine how the surfaces will affect the conformation of fibronectin, an important parameter in evaluating surface biocompatibility.

show abstract

Hierarchical scaffolds via combined macro- and micro-phase separation

Cited by 55 publications

References 12 publications

Fabrication of nanopatterned, porous microspheres using a glass capillary microfluidic device

Fabrication of nanopatterned, porous microspheres using a glass capillary microfluidic device

Modulating Protein Adhesion and Conformation with Block Copolymer Surfaces

Designing nanostructured block copolymer surfaces to control protein adhesion

Contact Info

Product

Resources

About