Hierarchical zwitterionic modification of a SERS substrate enables real-time drug monitoring in blood plasma

Sun, Fang; Hung, Hsiang‐Chieh; Sinclair, Andrew; Zhang, Peng; Bai, Tao; Galvan, Daniel David; Jain, Priyesh; Li, Bowen; Jiang, Shaoyi; Yu, Qiuming

doi:10.1038/ncomms13437

Cited by 181 publications

(134 citation statements)

References 45 publications

Supporting

Mentioning

131

Contrasting

Unclassified

Order By: Relevance

“…Excellent fouling resistance has been recently reported for ultra‐low fouling polymer brushes 2. Examples include nonionic poly(2‐hydroxyethyl methacrylate), poly(3‐hydroxypropyl methacrylate), poly( N ‐(2‐hydroxypropyl)methacrylamide) (pHPMAA), zwitterionic poly(carboxybetaine)‐based (pCB) brushes such as poly(carboxybetaine methacrylamide) (pCBMAA) and poly(carboxybetaine acrylamide) (pCBAA), or recently developed random copolymers combining zwitterionic and nonionic moieties such as p(CBMAA‐ co ‐HPMAA) 1,2,5,6. These platforms are characterized by diverse physicochemical surface properties including structure, architecture, thickness, rigidity, wettability, surface charge, packing density, or swelling properties; all of these parameters have been shown to have an impact on resulting surface resistance to fouling from complex media and biological activity of immobilized biorecognition elements 5,7.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Living Cell Behavior with Ultra‐Low Fouling Polymer Brush Interfaces

Víšová

Smolková

Uzhytchak

et al. 2020

Macromolecular Bioscience

View full text Add to dashboard Cite

Ultra‐low fouling and functionalizable coatings represent emerging surface platforms for various analytical and biomedical applications such as those involving examination of cellular interactions in their native environments. Ultra‐low fouling surface platforms as advanced interfaces enabling modulation of behavior of living cells via tuning surface physicochemical properties are presented and studied. The state‐of‐art ultra‐low fouling surface‐grafted polymer brushes of zwitterionic poly(carboxybetaine acrylamide), nonionic poly(N‐(2‐hydroxypropyl)methacrylamide), and random copolymers of carboxybetaine methacrylamide (CBMAA) and HPMAA [p(CBMAA‐co‐HPMAA)] with tunable molar contents of CBMAA and HPMAA are employed. Using a model Huh7 cell line, a systematic study of surface wettability, swelling, and charge effects on the cell growth, shape, and cytoskeleton distribution is performed. This study reveals that ultra‐low fouling interfaces with a high content of zwitterionic moieties (>65 mol%) modulate cell behavior in a distinctly different way compared to coatings with a high content of nonionic HPMAA. These differences are attributed mostly to the surface hydration capabilities. The results demonstrate a high potential of carboxybetaine‐rich ultra‐low fouling surfaces with high hydration capabilities and minimum background signal interferences to create next‐generation bioresponsive interfaces for advanced studies of living objects.

show abstract

Section: Introductionmentioning

confidence: 99%

Modulation of Living Cell Behavior with Ultra‐Low Fouling Polymer Brush Interfaces

Víšová

Smolková

Uzhytchak

et al. 2020

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…Thus, the sensing behavior of synthetic receptor assemblies on the devices' surfaces can be sensitively detected. Hung et al reported real-time monitoring of drugs in blood plasma by using SERS-based devices functionalized with SAMs for chemical sensing [44]. In addition, the surface of the prepared SERS device was also coated by a zwitterionic poly(carboxybetaine) layer (PCBAA) to reduce the non-specific adsorption of plasma proteins ( Figure 10).…”

Section: Transduction Of Sensing Information Based On "Invisible" Chamentioning

confidence: 99%

mentioning

confidence: 99%

The Power of Assemblies at Interfaces: Nanosensor Platforms Based on Synthetic Receptor Membranes

Minamiki

Ichikawa

Kurita

2020

Sensors

View full text Add to dashboard Cite

Synthetic sensing materials (artificial receptors) are some of the most attractive components of chemical/biosensors because of their long-term stability and low cost of production. However, the strategy for the practical design of these materials toward specific molecular recognition in water is not established yet. For the construction of artificial material-based chemical/biosensors, the bottom-up assembly of these materials is one of the effective methods. This is because the driving forces of molecular recognition on the receptors could be enhanced by the integration of such kinds of materials at the 'interfaces', such as the boundary portion between the liquid and solid phases. Additionally, the molecular assembly of such self-assembled monolayers (SAMs) can easily be installed in transducer devices. Thus, we believe that nanosensor platforms that consist of synthetic receptor membranes on the transducer surfaces can be applied to powerful tools for high-throughput analyses of the required targets. In this review, we briefly summarize a comprehensive overview that includes the preparation techniques for molecular assemblies, the characterization methods of the interfaces, and a few examples of receptor assembly-based chemical/biosensing platforms on each transduction mechanism.Synthetic receptors (i.e., artificial molecular recognition materials) are some of the most suitable platform materials for the development of chemical/biosensors owing to their chemical/physical stability, low cost of production, and their fine-tuning ability to select the required targets [4]. However, the preparation of artificial receptor-based sensors for practical applications is still in its initial stages, since the analyte specificity of most of the artificial materials is generally lower than that of biomaterials (i.e., antibodies and enzymes) [5]. To improve the binding affinity between the artificial receptors and the analytes, complicated synthesis procedures are required. Hence, a simpler way to improve the sensing ability of the artificial receptors should be considered to bring out the attractive features of these materials. To facilitate this, bottom-up integration of the receptors as molecular assemblies is considered as one of the most useful approaches for the construction of selective sensing fields for analytes. Moreover, the sensing features in the artificial receptor-based sensors could be finely tuned by using top-down technologies (e.g., molecular imprinting techniques [6], etc.) because the molecular recognition ability of the receptor assemblies depends on their nanostructures [7]. As aforementioned, the molecular assembly enhances the functions of a single kind of molecule ( Figure 1) [8]. In addition, the driving forces in molecular recognition (i.e., noncovalent interactions) can be amplified at the interfaces such as the boundary portion between the liquid and solid phases [9,10]. Thus, we believe that the installation of artificial receptors at the surface of the sensing portion in selective transducer...

show abstract

“…polyethyleneglycol or proteins. A recent report showed the hierarchical modification of the plasmonic transducer by a combination of a short probing ligand and a longer anti-fouling ligand, to detect doxorubicin spiked in whole blood at the micromolar range [122]. It should be noted that each application requires a different design and additional examples can be found in recent reviews focusing on the advances of SERS for in vivo diagnosis [107] and food safety [100].…”

Section: The Relevance Of Surface Chemistrymentioning

confidence: 99%

Colloidal design of plasmonic sensors based on surface enhanced Raman scattering

Hamon

Liz‐Marzán

2018

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

This feature article focuses on the use of colloid chemistry to engineer metallic nanostructures toward application in surface enhanced Raman scattering (SERS) sensing, in particular for 'real-life' applications, where the analyte may be present in complex mixtures. We present a broad summary of the field, including recent advances that have been developed during the past 10 years. Real-life applications require a rational design and we aimed at identifying the key elements involved in it. The discussion is centered around colloidal plasmonic nanoparticles and therefore we start from the library of morphologies that have been reported in the literature. To complete the picture, colloidal self-assembly, surface chemistry and the combination with materials science techniques are highlighted. Considering the progress in the field, SERS may ultimately realize its full potential as an ultrasensitive tool for routine analytical applications.

show abstract

Hierarchical zwitterionic modification of a SERS substrate enables real-time drug monitoring in blood plasma

Cited by 181 publications

References 45 publications

Modulation of Living Cell Behavior with Ultra‐Low Fouling Polymer Brush Interfaces

Modulation of Living Cell Behavior with Ultra‐Low Fouling Polymer Brush Interfaces

The Power of Assemblies at Interfaces: Nanosensor Platforms Based on Synthetic Receptor Membranes

Colloidal design of plasmonic sensors based on surface enhanced Raman scattering

Contact Info

Product

Resources

About