HIF‐1α regulates osteoclast activation and mediates osteogenesis during mandibular bone repair via CT‐1

Abstract

The treatment of bone malocclusion often requires a combination of orthodontics and orthognathic surgery. This surgery mainly involves several types of osteotomies to move the jaw block in order to correct a wide spectrum of dentofacial deformities (Patel & Novia, 2007). These osteotomies inevitably create a wedge-shaped osteotomy gap. Thus, bone gap repair is an important process after surgery. All patients of clinical orthodontic and orthognathic doctors expect an accelerated and well-done stable repair.Bone repair is a multistage and complicated process (Ghiasi et al., 2017) involving osteoclastic bone resorption and osteoblastic

LncRNA-mRNA Expression Profiles of Osteoclast After Conditional Knockout HIF-1α

“…In vivo, we bred HIF-1αflox/flox; Ctsk cre + mice to specifically knock out HIF-1α in osteoclasts. We found that the condyle was abnormal, and this phenotype was thoroughly studied in our previous work (Tian et al, 2020;Tang et al, 2020). In this study, we isolated and cultured monocytes from CKO and Ctrl mice to induce osteoclast differentiation.…”

“…We compared Cko mice with wild-type controls (Ctrl: HIF-1αflox/flox; Ctsk cre-) (n = 5 for each group). These methods were the same as those in our previous study ( Tian et al, 2020 ). All methods were performed in accordance with the revised Animals (Scientific Procedures) Act 1986 in the UK and Directive 2010/63/EU in Europe.…”

“…The medium was aspirated, and sodium hypochlorite was used to bleach the samples three times. Those methods are the same as our previous study ( Tian et al, 2020 ).…”

“…The protein bands were scanned and quantified by ImageJ software. Those methods are the same as our previous study ( Tian et al, 2020 ).…”

HIF-1α Regulates Bone Homeostasis and Angiogenesis, Participating in the Occurrence of Bone Metabolic Diseases

“…Then, IL-33 upregulates miR-34a-5p in a dose-dependent manner, followed by downregulation of osteoclast formation-related genes via the RANKL/Notch1 signal pathway, such as tartrate-resistant acid phosphatase (Trap), capthekin-K, nuclear factor of activated T cells c1 (NFATc1), and C-fos [ 34 ]. In addition, HIF-1α stimulates osteoclasts secreting Cardiotrophin-1 (CT-1), a gp130-signaling cytokine of the IL-6 superfamily, inducing BMSCs differentiating into osteoblast [ 35 , 36 , 37 , 38 ]. Chen et al [ 39 ] stably expressed HIF-1α by knocking out the VHL gene in osteocytes and found that it could boost the osteogenic differentiation of BMSCs and damage the osteoclast differentiation of BMMs.…”