High-accuracy fluence determination in ion beams using fluorescent nuclear track detectors

Osinga, Julia-Maria; Akselrod, M.S.; Herrmann, Rochus; Hable, V.; Dollinger, G.; Jäkel, Oliver; Greilich, Steffen

doi:10.1016/j.radmeas.2013.01.035

Cited by 29 publications

(31 citation statements)

References 7 publications

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“…Accordingly, FNTDs allow for high spatial resolution particle track visualization by confocal microscopy [6] (Figure 1b,c) and subsequent 3D particle track reconstruction [7,11]. The system is sensitive for ions with LET > 0.5 keV μ m −1 [5,8]. FNTD offers a detection efficiency close to 100% for the entire spectrum of primary particles and fragments at energies found in ion beam cancer therapy [5].…”

Section: Methodsmentioning

confidence: 99%

“…The system is sensitive for ions with LET > 0.5 keV μ m −1 [5,8]. FNTD offers a detection efficiency close to 100% for the entire spectrum of primary particles and fragments at energies found in ion beam cancer therapy [5]. The current limit of maximum accessible track fluence is in the range of 5 · 10 7 c m −2 [5], relevant to fluences providing clinical doses.…”

Section: Methodsmentioning

confidence: 99%

“…FNTDs based on Al 2 O 3 :C,Mg single crystals provide almost 100% detection efficiency of ion tracks imaged by confocal laser scanning microscope (CLSM) [5,6]. 3D information on energy deposition of ion tracks was obtained with diffraction-limited resolution and a multitude of physical parameters derived, such as location, direction, and possibly particle type and LET [6-9].…”

Section: Introductionmentioning

confidence: 99%

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Engineering cell-fluorescent ion track hybrid detectors

et al. 2013

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BackgroundThe lack of sensitive biocompatible particle track detectors has so far limited parallel detection of physical energy deposition and biological response. Fluorescent nuclear track detectors (FNTDs) based on Al2O3:C,Mg single crystals combined with confocal laser scanning microscopy (CLSM) provide 3D information on ion tracks with a resolution limited by light diffraction. Here we report the development of next generation cell-fluorescent ion track hybrid detectors (Cell-Fit-HD).MethodsThe biocompatibility of FNTDs was tested using six different cell lines, i.e. human non-small cell lung carcinoma (A549), glioblastoma (U87), androgen independent prostate cancer (PC3), epidermoid cancer (A431) and murine (VmDk) glioma SMA-560. To evaluate cell adherence, viability and conformal coverage of the crystals different seeding densities and alternative coating with extracellular matrix (fibronectin) was tested. Carbon irradiation was performed in Bragg peak (initial 270.55 MeV u−1). A series of cell compartment specific fluorescence stains including nuclear (HOECHST), membrane (Glut-1), cytoplasm (Calcein AM, CM-DiI) were tested on Cell-Fit-HDs and a single CLSM was employed to co-detect the physical (crystal) as well as the biological (cell layer) information.ResultsThe FNTD provides a biocompatible surface. Among the cells tested, A549 cells formed the most uniform, viable, tightly packed epithelial like monolayer. The ion track information was not compromised in Cell-Fit-HD as compared to the FNTD alone. Neither cell coating and culturing, nor additional staining procedures affected the properties of the FNTD surface to detect ion tracks. Standard immunofluorescence and live staining procedures could be employed to co-register cell biology and ion track information.ConclusionsThe Cell-Fit-Hybrid Detector system is a promising platform for a multitude of studies linking biological response to energy deposition at high level of optical microscopy resolution.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Engineering cell-fluorescent ion track hybrid detectors

et al. 2013

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“…One such implementation for biomedical radiation research is the Cell Fluorescent Ion Track Hybrid Detector (Cell-FIT-HD) biomedical sensor used for in situ biodosimetric detection of single ion traversals in cells irradiated with heavy ions (Fig.1) [5]. The biomedical sensor is based on a Landauer Al2O3:C,Mg FNTD [6,7]. Cells are grown on the FNTD and irradiated with ions and their response is then analysed.…”

Section: Introductionmentioning

confidence: 99%

Carbon ion dosimetry on a fluorescent nuclear track detector using widefield microscopy

Walsh

Liew

Schlegel

et al. 2020

Preprint

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Fluorescent nuclear track detectors (FNTD) are solid-state dosimeters used in a wide range of dosimetric and biomedical applications in research worldwide. FNTD's are a core but currently underutilized dosimetry tool in the field of radiation biology which are inherently capable of visualizing the tracks of ions used in hadron therapy. The ions that traverse the FNTD deposit their energy according to their linear energy transfer (LET) and transform colour centres to form trackspots around their trajectory. These trackspots are the fingerprints of the ions which have fluorescent properties (620 nm peak excitation and 750 nm emission). These properties enable an analysis of the trackspots in the FNTD using fluorescence microscopy enables a well-defined dosimetric readout with a spatial component indicating the trajectory of individual ions. The current method used to analyse the FNTDs is laser scanning confocal microscopy (LSM). This imaging technique enables a precise localization and imaging of track spots in x, y and z however due to the scanning of a single laser spot across the sample requires a lot of time for large samples. This body of work conclusively shows for the first time that the readout of the trackspots present after irradiation (0.5 Gy carbon ions) in the FNTD can be captured with a widefield microscope (WF). This method was developed to optimize the readout of the Cell-FIT-HD biomedical sensor based on a Landauer Al2O3:C,Mg FNTD which is used for biological single cell dosimetry and tracking using widefield microscopy. The cells to be analysed are seeded onto an FNTD and irradiated with carbon ions, then the FNTD and the cells can be imaged using an LSM and the WF microscope respectively. The capture of the two separate readouts, cells and tracks, on two separate microscopes is not only time intensive but also introduces a spatial error between tracks and cells which needs to be corrected. The work in this paper now enables the use of a single widefield microscope to image the cells growing on the FNTD and the tracks in the FNTD. In comparison to the current LSM system the WF readout of the FNTD is a factor ~10 faster, for an area 2.97 times the size making the method nearly a factor 19 faster in track acquisition. This WF setup will replace LSM in the Cell-FIT-HD for FNTD track readout, which will enable a higher throughput of samples as well as a reduced error in the correlation between tracks and cells and enable a high throughput readout of Cell-FIT-HD.

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“…et al [13] used Al 2 O 3 :C, Mg-based fluorescent nuclear track detectors-FNTDs and confocal laser scanning microscopy as a semiautomatic tool for fluence measurements in clinical ion beams. Then Firas M. Al-Jomaily, et al [14], used MATLAB program to determine the nuclear track parameters such as track diameter-D T (μm), number of track-N T and track area-A T for LR-115 detector which irradiated by alpha particle.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Nuclear Track Parameters of CN-85 Detector Irradiated to Thermal Neutrons by Using MATLAB Program

Al-Jobouri¹,

Rajab²,

Najam³

2015

Detection

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CN-85 detector which covered with boric acid H 3 Bo 3 pellete has been irradiated by thermal neutrons from ( 241 Am-9 Be) source with activity 12 Ci and neutron flux 10 5 n. cm −2 . s −1 . The irradiation times-T D for detector were 4 h, 8 h, 16 h and 24 h. The track detector has been etched with sodium hydroxide. After chemical etching of the irradiated CN-85 detector, the images have been taken from a digital camera connected to the optical microscope. Image processing for the output images has been performed using MATALB program, and these images were analyzed and we had found the following relations: a) The relation between summation of opened track or surface density for tracks (intensi- 0.4225, 0.845, 1.2675 and 1.69 µm). The study indicates the possibility of using the analysis of image processing to CN-85 detector for classification of α-particle emitters through limitation of radius of track-R T , in addition to the contribution of these techniques in preparation of nano-filters and nono-membrane in nanotechnology fields. ty-IT) varies with radius of opening (track radius-R T ). b) The relation between the tracks number-N T varies with the tracks diameter-D T (in micrometer) and tracks area-A T . That analysis of image processing was obtained, and the track intensity-I T was decreased with increase of track radius-R T at all of the irradiation time-T D . And the track intensity-I T was increased with increasing irradiation time-T D (h) for different track radius-R T (

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High-accuracy fluence determination in ion beams using fluorescent nuclear track detectors

Cited by 29 publications

References 7 publications

Engineering cell-fluorescent ion track hybrid detectors

Engineering cell-fluorescent ion track hybrid detectors

Carbon ion dosimetry on a fluorescent nuclear track detector using widefield microscopy

Analysis of Nuclear Track Parameters of CN-85 Detector Irradiated to Thermal Neutrons by Using MATLAB Program

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