High-affinity neurotensin receptor is involved in phosphoinositide hydrolysis stimulation by carbachol in neonatal rat brain

Pereyra-Alfonso, Susana; Ordieres, Marı́a Graciela López; Armanino, María del Valle; Arnaiz, Georgina Rodrı́guez de Lores

doi:10.1016/j.devbrainres.2004.11.003

Cited by 4 publications

(5 citation statements)

References 48 publications

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“…In the present work exogenous neurotensin was not added during prisms incubation, and therefore, the effect of SR 48692 would be most likely attributable to the interaction of endogenous neurotensin with its receptor. This finding is in line with previous results indicating that endogenous neurotensin is involved in PI hydrolysis increase by carbachol [7]. It could be hypothesized that a change in endogenous neurotensin occurs due to the presence of SR 48692.…”

Section: Discussionsupporting

confidence: 91%

“…, K ? -ATPase inhibitor (present findings) or by a cholinergic muscarinic agonist like carbachol [7].…”

Section: Discussionmentioning

confidence: 78%

“…However, the single addition of the peptide fails to alter basal IP accumulation. Taken jointly, results suggested that endogenous neurotensin may be involved in this effect [7].…”

Section: Discussionmentioning

confidence: 98%

“…In addition, the high-affinity neurotensin (NTS1) receptor, a G-protein receptor involved in IP accumulation [4], is present in brain at birth and peaks at day seven [5]. Experimental evidence points to a relationship between cholinergic and neurotensinergic systems; accordingly, PI hydrolysis induced by carbachol, either alone or plus neurotensin is partially or totally blocked by SR 48692, a non-peptidic antagonist for neurotensin (NTS1) receptor [6], suggesting the involvement of this receptor in carbachol effect [7].…”

Section: Introductionmentioning

confidence: 97%

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High-affinity Neurotensin Receptor is Involved in Phosphoinositide Turnover Increase by Inhibition of Sodium Pump in Neonatal Rat Brain

et al. 2008

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Phosphoinositide (PI) metabolism is enhanced in neonatal brain by activation of neurotransmitter receptors and by inhibition of the sodium pump with ouabain or endogenous inhibitor termed endobain E. Peptide neurotensin inhibits synaptosomal membrane Na(+), K(+)-ATPase activity, an effect blocked by SR 48692, a selective antagonist for high-affinity neurotensin receptor (NTS1). The purpose of this study was to evaluate potential participation of NTS1 receptor on PI hydrolysis enhancement by sodium pump inhibition. Cerebral cortex miniprisms from neonatal Wistar rats were preloaded with [(3)H]myoinositol in buffer during 60 min and further preincubated for 0 min or 30 min in the absence or presence of SR 48692. Then, ouabain or endobain E were added and incubation proceeded during 20 or 60 min. Reaction was stopped with chloroform/methanol and [(3)H]inositol-phosphates (IPs) accumulation was quantified in the water phase. After 60-min incubation with ouabain, IPs accumulation values reached roughly 500% or 860% in comparison with basal values (100%), if the preincubation was omitted or lasted 30 min, respectively. Values were reduced 50% in the presence of SR 48692. In 20-min incubation experiments, IPs accumulation by ouabain versus basal was 300% or 410% if preincubation was 0 min or 30 min, respectively, an effect blocked 23% or 32% with SR 48692. PI hydrolysis enhancement by endobain E was similarly blocked by SR 48692, being this effect higher when sample incubation with the endogenous inhibitor lasted 60 min versus 20 min. Present results indicate that PI hydrolysis increase by sodium pump inhibition with ouabain or endobain E is partially diminished by SR 48692. It is therefore suggested that NTS1 receptor may be involved in cell signaling system mediated by PI turnover.

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Section: Discussionsupporting

confidence: 91%

“…, K ? -ATPase inhibitor (present findings) or by a cholinergic muscarinic agonist like carbachol [7].…”

Section: Discussionmentioning

confidence: 78%

“…However, the single addition of the peptide fails to alter basal IP accumulation. Taken jointly, results suggested that endogenous neurotensin may be involved in this effect [7].…”

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

High-affinity Neurotensin Receptor is Involved in Phosphoinositide Turnover Increase by Inhibition of Sodium Pump in Neonatal Rat Brain

et al. 2008

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“…-ATPase inhibitor, termed endobain E, which shares several biological properties with ouabain. They include the blockade of high affinity [ 3 H]ouabain binding [42], norepinephrine release enhancement [43] and uptake decrease [44] from rat hypothalamus slices, excitatory amino acid release enhancement from synaptosomes [19], as well as phosphoinositide turnover increase [45].…”

Section: Discussionmentioning

confidence: 99%

Intracerebroventricular Administration of Ouabain to Rats Changes the Expression of NMDA Receptor Subunits in Cerebral Cortex and Hippocampus

Bersier

Arnaiz

2009

Neurochem Res

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Ouabain exerts neurotoxic action and activates the population of NMDA receptors. Herein the effect of ouabain on the expression of NMDA subunits was evaluated. Adult Wistar rats were administered intracerebroventricularly with 0.1, 10 and 100 nmol ouabain or saline solution (control). Two days later, membranes of cerebral cortex and hippocampus were isolated. Western blots with antibodies for the NMDA receptor subunits: NR1; NR2A; NR2B; NR2C and NR2D were carried out. In cerebral cortex, NR2D subunit increased 30% with 10 nmol ouabain dose. With 100 nmol ouabain, NR1 and NR2D subunits enhanced 40 and 20%, respectively. In hippocampus, with the dose of 0.1 nmol ouabain, NR1 subunit enhanced roughly 50% whereas NR2B subunit decreased 30%. After administration of 10 nmol ouabain dose, NR2A, NR2B and NR2C subunits decreased 40, 50 and 30%, respectively. With the dose of 100 nmol of ouabain, NR1, NR2A and NR2B subunits diminished 10-20%. It is concluded that ouabain administration led to a differential regulation in the expression of NMDA subunits. These results may be correlated with the modulatory action of ouabain on NMDA receptor.

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Phosphoinositide hydrolysis increase by angiotensin-(1–7) in neonatal rat brain

Pereyra-Alfonso

Arnaiz

Peña

2007

Regulatory Peptides

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High-affinity neurotensin receptor is involved in phosphoinositide hydrolysis stimulation by carbachol in neonatal rat brain

Cited by 4 publications

References 48 publications

High-affinity Neurotensin Receptor is Involved in Phosphoinositide Turnover Increase by Inhibition of Sodium Pump in Neonatal Rat Brain

High-affinity Neurotensin Receptor is Involved in Phosphoinositide Turnover Increase by Inhibition of Sodium Pump in Neonatal Rat Brain

Intracerebroventricular Administration of Ouabain to Rats Changes the Expression of NMDA Receptor Subunits in Cerebral Cortex and Hippocampus

Phosphoinositide hydrolysis increase by angiotensin-(1–7) in neonatal rat brain

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