There are five types of resistant starch and the most commonly used as functional food is type-III resistant starch (RS-III). RS-III is a retrograded of gelatinized starch that is conventionally produced by heating and cooling treatment. This study characterized the RS-III produced by an unconventional method by modification of high shear mixing (HSM) combined with membrane microfiltration from cassava starch. In this research, a starch/water mixture with a concentration of 1/20 w/v was gelatinized in the HSM reactor at 95°C for 15 minutes and then separated using membrane microfiltration. The separated permeate was cooled to the retrogradation process. The products were characterized by iodine, X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), and Total Dietary Fiber (TDF) content. From the experiment, it was found that the higher HSM speed significantly increased the TDF content up to 12.33% and decreased the amylose content and crystallinity of the RS-III products.