High and low molecular weight kininogen and plasma prekallikrein/plasma kallikrein in villous capillaries of human term placenta

Hermann, Andrea; Buchinger, Peter; Somlev, B; Rhebock, J.

doi:10.1016/s0143-4004(96)90042-9

Cited by 25 publications

(28 citation statements)

References 29 publications

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“…Our results confirm the reports of Ciechanowicz et al (1993) and Hermann et al (1996) and demonstrate that the PPK gene is transcribed in an even larger variety of tissues and cells than reported by these authors. Inferring from the results with epithelial cell lines, not only ubiquitously occurring cells are the origin of PPK mRNA but also tissuespecific cells.…”

Section: Possible Physiological Functions Of Extrahepatically Synthessupporting

confidence: 93%

“…The previous results (Ciechanowicz et al, 1993;Hermann et al, 1996) did not indicate whether PPK mRNA is expressed by ubiquitous cells like fibroblasts, endothelial cells, and white blood cells, or by tissue-specific cells. Therefore, in order to clarify this issue and to find a model system for investigating the physiological significance of extrahepatic PPK synthesis, we undertook a study on PPK mRNA expression in cultured human fibroblasts and epithelial cells originating from different human tissues.…”

Section: Introductionmentioning

confidence: 71%

“…Direct evidence of hepatic PPK synthesis came from immunocytochemical studies using confocal laser scanning microscopy which demonstrated PPK in hepatocytes (Henderson et al, 1992). Recently, Ciechanowicz et al (1993) and our group (Hermann et al, 1996) provided evidence for extrahepatic synthesis of PPK: by employing RT-PCR, expression of PPK mRNA was detected in human liver, kidney, adrenal gland and placenta. The aim of the present study was to clarify whether PPK mRNA is expressed in tissues other than the liver and in tissue-specific cells, and to find a model for studying extrahepatic PPK synthesis and its regulation.…”

Section: Expression Of Ppk Mrna In Nonhepatic Tissues and Cell Linesmentioning

confidence: 99%

“…Recently, Ciechanowicz et al (1993) and our group (Hermann et al, 1996) found by reverse transcription PCR that PPK mRNA is present in RNA preparations from whole tissues of kidney, adrenal gland and placenta. Thus, in contrast to the generally accepted concept that plasma kallikrein is synthesized solely in the liver, one has to conclude that synthesis of PPK also takes place outside the liver and that the enzyme may have special, hitherto unknown local function(s).…”

Section: Introductionmentioning

confidence: 96%

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Expression of Plasma Prekallikrein mRNA in Human Nonhepatic Tissues and Cell Lineages Suggests Special Local Functions of the Enzyme

Hermann¹,

Arnhold²,

Kresse³

et al. 1999

Biological Chemistry

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At present it is generally accepted that plasma prekallikrein (PPK) is synthesized in the liver and secreted into the bloodstream. Surprisingly, it has recently been shown that PPK mRNA is present also in RNA from the kidney, adrenal gland and placenta. In spite of its novelty and possible important physiological implications this finding has been neglected. Here we report that PPK mRNA is expressed also in the human brain, heart, lung,

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Section: Possible Physiological Functions Of Extrahepatically Synthessupporting

confidence: 93%

Section: Introductionmentioning

confidence: 71%

Section: Expression Of Ppk Mrna In Nonhepatic Tissues and Cell Linesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Expression of Plasma Prekallikrein mRNA in Human Nonhepatic Tissues and Cell Lineages Suggests Special Local Functions of the Enzyme

Hermann¹,

Arnhold²,

Kresse³

et al. 1999

Biological Chemistry

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“…The molecules circulate as a complex either free or attached to the external surface of neutrophils (Schapira et al, 1982;Henderson et al, 1994). Experimental evidence gathered from RT-PCR studies clearly suggested PPK mRNA expression in several extra-hepatic tissues (Ciechanowicz et al, 1993;Hermann et al, 1996Hermann et al, , 1999. Recently we confirmed and extended these studies and demonstrated by quantitative RT-PCR the expression of PPK mRNA in 16 human tissues (Figure 1, Neth et al, 2001).…”

supporting

confidence: 63%

Cellular expression of plasma prekallikrein in human tissues

Fink

Bhoola

Snyman

et al. 2007

BCHM

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Plasma prekallikrein (PPK) is synthesised in hepatocytes and secreted into the blood, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Recently we demonstrated by quantitative RT-PCR that the human PPK gene is transcribed not only in the liver, but also in various non-hepatic human tissues at significant levels. However, up to now no reliable information is available concerning protein synthesis in the corresponding human tissues. Here we demonstrate by immunohistochemical studies that PPK or plasma kallikrein (PK) is localised in cells of different embryologically derived human tissues. In the human nephron, single cells of the distal tubules stained intensely, while the cytoplasm of cells forming proximal tubules and collecting ducts stained uniformly. PPK/PK was localised in hepatic epithelial cells of the liver, in cells of the pancreatic islet of Langerhans, in the interstitial Leydig cells of the testes, in the follicular and thecal granulosa cells of the ovary, and in the parotid gland, oesophagus, skin, respiratory tract, prostate and breast. We conclude that the cellular localisation of PPK/PK in multiple different progenitorderived cells indicates specific cellular functions of this enzyme, in addition to its known function in the blood.

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Differential processing of high‐molecular‐weight kininogen during normal pregnancy

Droll

Hsu²,

Drake

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Studies identified kininogen as a potential biomarker of preeclampsia, a major cause of adverse maternal outcomes. High‐molecular‐weight kininogen (HK) and its activated form participate in numerous pathways associated with establishing and maintaining pregnancy. However, dynamic changes in HK and naturally occurring HK‐derived peptides during the natural course of pregnancy are largely unknown. Methods Longitudinal serum samples during the course of normal pregnancy (trimesters T1, T2, T3) from 60 pregnant women were analyzed by western blot with an anti‐HK antibody. Circulating peptides in longitudinal serum specimens derived from 50 participants were enriched using nanoporous silica thin films. Peptides were identified by liquid chromatography/tandem mass spectrometry (LC/MS/MS) and database searching. Relative quantification was performed using MaxQuant and in‐house scripts. Normality was evaluated by either ANOVA or Friedman tests with p < 0.05 for statistical significance. Results Western blotting revealed that HK significantly decreased during normal pregnancy (T1 vs T2, p < 0.05; T1 vs T3, p < 0.0001). A 100 kDa intermediate increased during pregnancy (T1 vs T2, p < 0.005; T1 vs T3, p < 0.01). Moreover, the heavy chain (T1 vs T2, p < 0.0001; T1 vs T3, p < 0.0001; T2 vs T3, p < 0.01) and light chain (T1 vs T2, p < 0.0001; T1 vs T3, p < 0.0001; T2 vs T3, p < 0.05) significantly increased during pregnancy. LC/MS/MS analysis identified 180 kininogen‐1 peptides, of which 167 mapped to domain 5 (D5). Seventy‐three peptides with ten or more complete data sets were included for further analysis. Seventy peptides mapped to D5, and 3, 24, and 43 peptides showed significant decrease, no trend, and significant increase, respectively, during pregnancy. Conclusions This study demonstrates dynamic changes in HK and naturally occurring HK‐derived peptides during pregnancy. Our study sheds light on the gestational changes of HK and its peptides for further validation of them as potential biomarkers for pregnancy‐related complications.

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High and low molecular weight kininogen and plasma prekallikrein/plasma kallikrein in villous capillaries of human term placenta

Cited by 25 publications

References 29 publications

Expression of Plasma Prekallikrein mRNA in Human Nonhepatic Tissues and Cell Lineages Suggests Special Local Functions of the Enzyme

Expression of Plasma Prekallikrein mRNA in Human Nonhepatic Tissues and Cell Lineages Suggests Special Local Functions of the Enzyme

Cellular expression of plasma prekallikrein in human tissues

Differential processing of high‐molecular‐weight kininogen during normal pregnancy

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