High antibody titres in mice with polymethylmethacrylate nanoparticles as adjuvant for HIV vaccines

Stieneker, F; Kreuter, Jörg; Löwer, Johannes

doi:10.1097/00002030-199104000-00012

Cited by 49 publications

(15 citation statements)

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“…Nevertheless, PMMA is a biomaterial that has already been widely used in orthopedic implant devices. Furthermore, PMMA nanoparticles have been used for DNA vaccination by the route of intramuscular injection and already been shown to be nontoxic for the cells and well tolerated in vivo 22–28. It might be argued that PMMA particle preparation was not a new technique, but, to our knowledge, this is the first report to show that the gene gun can deliver DNA vaccine by propelling PMMA particles mixed with DNA.…”

Section: Discussionmentioning

confidence: 98%

“…Furthermore, PMMA nanoparticles have been used for DNA vaccination by the route of intramuscular injection and already been shown to be nontoxic for the cells and well tolerated in vivo. [22][23][24][25][26][27][28] It might be argued that PMMA particle preparation was not a new technique, but, to our knowledge, this is the first report to show that the gene gun can deliver DNA vaccine by propelling PMMA particles mixed with DNA. The best result was PMMA 4 particles with the size of 460 6 160 nm and surface charge of 11.5 6 1.8 mV.…”

Section: Discussionmentioning

confidence: 99%

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PMMA particle‐mediated DNA vaccine for cervical cancer

Lou

Cheng

Chung

et al. 2008

J Biomedical Materials Res

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DNA vaccination is a novel immunization strategy that possesses many potential advantages over other vaccine strategies. One of the major difficulties hindering the clinical application of DNA vaccination is the relative poor immunogenicity of DNA vaccines. Poly(methyl methacrylate) (PMMA) is a synthetic polymer approved by the Food and Drug Administration for certain human clinical applications such as the bone cement. In vivo, PMMA particles are phagocytosable and have the potential to initiate strong immune responses by stimulating the production of inflammatory cytokines. In this study, we synthesized a series of PMMA particles (PMMA 1-5) with different particle sizes and surface charges to test the feasibility of implementing such polymer particles for DNA vaccination. To our knowledge, this is the first report to show that the gene gun can deliver DNA vaccine by propelling PMMA particles mixed with plasmid DNA for cervical cancer. It was found that PMMA 4 particles (particle size: 460 +/- 160 nm, surface charge: +11.5 +/- 1.8 mV) stimulated the highest level of TNF-alpha production by macrophages in vitro and yielded the best result of antitumor protection in vivo. Therefore, our results possess the potential for translation and implementation of polymer particles in gene gun delivering DNA vaccination.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

PMMA particle‐mediated DNA vaccine for cervical cancer

Lou

Cheng

Chung

et al. 2008

J Biomedical Materials Res

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“…A number of studies describe the use of nanoparticles as adjuvants. For example, polymethylmethacrylate nanoparticles induced long-lasting antibody titres (the quantity of substance required to produce a reaction) .The antibody response was 100 times higher than when a traditional aluminium sulphate (alum) adjuvant was used (Stieneker et al 1991).…”

Section: Adjuvant Propertiesmentioning

confidence: 99%

Nanodelivery: An Emerging Avenue for Nutraceuticals and Drug Delivery

Aklakur

Rather

Kumar

2015

Critical Reviews in Food Science and Nutrition

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Aquaculture has been globally recognized as the fastest growing food production sector which plays a major role in meeting the increasing demand for animal protein requirement. A consensus is growing that a dramatic increase in aquaculture is needed to supply future aquatic food needs. However, there are sustained problems with the aquaculture like disease outbreaks, chemical pollution, the environmental destruction, and inefficient feed utilization. These altogether raise question mark on sustainability of aquaculture. In spite of the several strategy adopted on national and international level, as improved laboratory facilities, diagnostic expertise, and control and therapeutic strategies in order to handle disease outbreaks more effectively. Aquaculture industry is under uncertainty and the progress has not matched that of the rapidly developing aquaculture sector. In order to control disease prevalence and ensure better health of system and sustainable production, the sector demand more technical innovation for the drug use, disease treatment, water quality management, production of tailored fish for suiting better health, productivity drive by epigenetic and nutrigenomic interaction, better breeding success by efficient delivery of maturation and spawning inducing agent, nutraceutical delivery for rapid growth promotion and culture time reduction, successful use of autotransgenic, and effective vaccine. Nanotechnology has a tremendous potential to revolutionize agriculture and allied fields including aquaculture and fisheries. For these multiple purposes effort, importance of nanotechnology and nanodelivery of drugs, vaccine, nutraceutical, inducing hormones, and growth-promoting anabolics open tremendous opportunity. The paper has been targeted to delineate the possible future application of nanodelivery for the aquaculture development.

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“…Polymethylmethacrylate nanoparticles have been shown to induce long-lasting antibody titers in HIV-2 whole virus vaccine in mice and the antibody response was 100-fold higher than that of standard adjuvant [131]. A number of studies have reported on the adjuvant properties of nanoparticles.…”

Section: Immune Stimulationmentioning

confidence: 99%