High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants

Demirer, Gözde S.; Zhang, Huan; Matos, Juliana L.; Goh, Natalie S.; Cunningham, F. J.; Sung, Younghun; Chang, Roger; Aditham, Abhishek; Chio, Linda; Cho, Myeong‐Je; Staskawicz, Brian J.; Landry, Markita P.

doi:10.1038/s41565-019-0382-5

Cited by 468 publications

(428 citation statements)

References 52 publications

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“…Structurally, AQPs are similar to cell wall pores. The transport carrier proteins and ion transporters may also participate in the movement of NPs through the cell wall and plasma membrane . Tani and Barrington reported significant role of ion transporters in the movement of NPs in the root cells .…”

Section: Uptake Of Nps By Plantsmentioning

confidence: 99%

Nanoparticle–Plant Interactions: Two‐Way Traffic

Khan

Adam

Rizvi

et al. 2019

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167

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In this Review, an effort is made to discuss the most recent progress and future trend in the two‐way traffic of the interactions between plants and nanoparticles (NPs). One way is the use of plants to synthesize NPs in an environmentally benign manner with a focus on the mechanism and optimization of the synthesis. Another way is the effects of synthetic NPs on plant fate with a focus on the transport mechanisms of NPs within plants as well as NP‐mediated seed germination and plant development. When NPs are in soil, they can be adsorbed at the root surface, followed by their uptake and inter/intracellular movement in the plant tissues. NPs may also be taken up by foliage under aerial deposition, largely through stomata, trichomes, and cuticles, but the exact mode of NP entry into plants is not well documented. The NP–plant interactions may lead to inhibitory or stimulatory effects on seed germination and plant development, depending on NP compositions, concentrations, and plant species. In numerous cases, radiation‐absorbing efficiency, CO2 assimilation capacity, and delay of chloroplast aging have been reported in the plant response to NP treatments, although the mechanisms involved in these processes remain to be studied.

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Section: Uptake Of Nps By Plantsmentioning

confidence: 99%

Nanoparticle–Plant Interactions: Two‐Way Traffic

Khan

Adam

Rizvi

et al. 2019

Small

167

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“…Single‐walled carbon nanotubes of high aspect ratios were functionalized with PEI, to electrostatically hold the DNA and offload it in a weakly basic environment . The delivery of DNA‐PEI‐CNTs complex into the mature leaf cells was confirmed by electron microscopy; NIR fluorescence imaging showed that this cargo carrier was 700 times more efficient than pDNA adsorbed on pristine SWCNTs.…”

Section: Introductionmentioning

confidence: 99%

Derivatized Carbon Nanotubes for Gene Therapy in Mammalian and Plant Cells

et al. 2020

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The concept of gene vectors for therapeutic applications has been known for several years, but it is far from revealing its actual potential. With the advent of hollow cylindrical carbon nanomaterials such as carbon nanotubes (CNTs), researchers have invented several new tools to deliver genes at the required site of action in mammalian and plant cells. The ease of diversified functionalization has allowed CNTs to be by far the most adaptable non‐viral vector for gene therapy. This Minireview addresses the dexterity with which CNTs undergo surface modifications and their applications as a potent vector in gene therapy of humans and plants. Specifically, we will discuss the new tools that scientific communities have invented to achieve gene therapy using plasmid DNA, RNA silencing, suicide gene therapy, and plant genetic engineering. Additionally, we will shed some light on the mechanism of gene transportation using carbon nanotubes in cancer cells and plants.

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“…Our work focuses on single-walled carbon nanotubes (SWCNTs), a nanoparticle class that possesses unique optical and physical properties ideal for biological imaging, molecular sensing, and bio-delivery applications. [25][26][27] SWCNTs are composed of a graphitic lattice structure in a high aspect ratio, cylindrical geometry, resulting in an intrinsic near-infrared (nIR) bandgap fluorescence over which biological samples are optically transparent, 28 with no photobleaching threshold. 29 This pristine carbon structure renders the surface hydrophobic, and SWCNTs accordingly exist as aggregated bundles in aqueous solution.…”

Section: Introductionmentioning

confidence: 99%

Protein Corona Composition and Dynamics on Carbon Nanotubes in Blood Plasma and Cerebrospinal Fluid

Pinals¹,

Yang²,

Rosenberg

et al. 2020

Preprint

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When a nanoparticle enters a biological environment, the surface is rapidly coated with proteins to form a "protein corona". Presence of the protein corona surrounding the nanoparticle has significant implications for applying nanotechnologies within biological systems, affecting outcomes such as biodistribution and toxicity. Herein, we measure protein corona formation on single-stranded DNA wrapped single-walled carbon nanotubes (ssDNA-SWCNTs), a high-aspect ratio nanoparticle ideal for sensing and delivery applications, and polystyrene nanoparticles, a model nanoparticle system. The protein corona of each nanoparticle is studied in human blood plasma and cerebrospinal fluid. We characterize corona composition by proteomic mass spectrometry to determine abundant and differentially enriched vs. depleted corona proteins. Highbinding corona proteins on ssDNA-SWCNTs include proteins involved in lipid binding and transport (clusterin and apolipoprotein A-I), complement activation (complement C3), and blood coagulation (fibrinogen). Of note, albumin is the most common blood protein (55% w/v), yet exhibits low-binding affinity towards ssDNA-SWCNTs, displaying 1300-fold lower bound concentration relative to native plasma. We investigate the role of electrostatic and entropic interactions driving selective protein corona formation, and find that hydrophobic interactions drive inner corona formation, while shielding of electrostatic interactions allows for outer corona formation. Lastly, we study real-time binding of proteins on ssDNA-SWCNTs and find relative agreement between proteins that are enriched and bind strongly, such as fibrinogen, and proteins that are depleted and bind marginally, such as albumin. Interestingly, certain proteins express contrary behavior in single-protein experiments than within the whole biofluid, highlighting the importance of cooperative mechanisms driving selective corona adsorption on the SWCNT surface. Knowledge of the protein corona composition, dynamics, and structure informs translation of engineered nanoparticles from in vitro design to effective in vivo application.

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High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants

Cited by 468 publications

References 52 publications

Nanoparticle–Plant Interactions: Two‐Way Traffic

Nanoparticle–Plant Interactions: Two‐Way Traffic

Derivatized Carbon Nanotubes for Gene Therapy in Mammalian and Plant Cells

Protein Corona Composition and Dynamics on Carbon Nanotubes in Blood Plasma and Cerebrospinal Fluid

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